Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   E3S85_RS02380 Genome accession   NZ_CP047839
Coordinates   448040..448537 (+) Length   165 a.a.
NCBI ID   WP_001794328.1    Uniprot ID   -
Organism   Staphylococcus aureus strain UP_678     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 443040..453537
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E3S85_RS02340 (E3S85_02340) - 443084..444070 (+) 987 WP_000161314.1 ROK family glucokinase -
  E3S85_RS02345 (E3S85_02345) - 444070..444399 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  E3S85_RS02350 (E3S85_02350) - 444396..445019 (+) 624 WP_160175980.1 MBL fold metallo-hydrolase -
  E3S85_RS02355 (E3S85_02355) comGA 445071..446045 (+) 975 WP_160175981.1 competence type IV pilus ATPase ComGA Machinery gene
  E3S85_RS02360 (E3S85_02360) comGB 446017..447087 (+) 1071 WP_000776412.1 competence type IV pilus assembly protein ComGB Machinery gene
  E3S85_RS02365 (E3S85_02365) comGC 447101..447412 (+) 312 WP_000472257.1 competence type IV pilus major pilin ComGC Machinery gene
  E3S85_RS02370 (E3S85_02370) comGD 447390..447836 (+) 447 WP_160175982.1 competence type IV pilus minor pilin ComGD Machinery gene
  E3S85_RS02375 (E3S85_02375) comGE 447823..448122 (+) 300 WP_000844413.1 hypothetical protein Machinery gene
  E3S85_RS02380 (E3S85_02380) comGF 448040..448537 (+) 498 WP_001794328.1 competence type IV pilus minor pilin ComGF Machinery gene
  E3S85_RS02385 (E3S85_02385) - 448634..448780 (+) 147 WP_001789879.1 hypothetical protein -
  E3S85_RS02390 (E3S85_02390) - 448770..449294 (+) 525 WP_001015125.1 shikimate kinase -
  E3S85_RS02395 (E3S85_02395) gcvT 449453..450544 (+) 1092 WP_000093347.1 glycine cleavage system aminomethyltransferase GcvT -
  E3S85_RS02400 (E3S85_02400) gcvPA 450564..451910 (+) 1347 WP_000019697.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  E3S85_RS02405 (E3S85_02405) gcvPB 451903..453375 (+) 1473 WP_000202178.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19197.95 Da        Isoelectric Point: 10.2192

>NTDB_id=417552 E3S85_RS02380 WP_001794328.1 448040..448537(+) (comGF) [Staphylococcus aureus strain UP_678]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDRNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNLQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=417552 E3S85_RS02380 WP_001794328.1 448040..448537(+) (comGF) [Staphylococcus aureus strain UP_678]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTAATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAGAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT
AAAACAATTTATGTATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)

Putative Competence protein ComGF (ComGF)

(68-155)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus N315

98.788

100

0.988
  comGF Staphylococcus aureus MW2

97.576

100

0.976

Multiple sequence alignment