Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   H9445_RS16435 Genome accession   NZ_CP060709
Coordinates   3330106..3330765 (+) Length   219 a.a.
NCBI ID   WP_001221495.1    Uniprot ID   P52076
Organism   Escherichia coli strain ST18     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 3325106..3335765
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H9445_RS16410 (H9445_16410) ygiS 3326389..3327996 (-) 1608 WP_001295629.1 ABC transporter substrate-binding protein -
  H9445_RS16415 (H9445_16415) mqsA 3328129..3328524 (-) 396 WP_000650107.1 type II toxin-antitoxin system antitoxin MqsA -
  H9445_RS16420 (H9445_16420) mqsR 3328526..3328822 (-) 297 WP_000415584.1 type II toxin-antitoxin system toxin MqsR -
  H9445_RS16425 (H9445_16425) ygiV 3329027..3329509 (-) 483 WP_000183505.1 GyrI-like domain-containing protein -
  H9445_RS16430 (H9445_16430) ygiW 3329562..3329954 (-) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  H9445_RS16435 (H9445_16435) ciaR 3330106..3330765 (+) 660 WP_001221495.1 quorum sensing response regulator transcription factor QseB Regulator
  H9445_RS16440 (H9445_16440) qseC 3330762..3332111 (+) 1350 WP_000673402.1 quorum sensing histidine kinase QseC -
  H9445_RS16445 (H9445_16445) ygiZ 3332157..3332489 (-) 333 WP_000917684.1 DUF2645 family protein -
  H9445_RS16450 (H9445_16450) mdaB 3332808..3333389 (+) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  H9445_RS16455 (H9445_16455) ygiN 3333420..3333734 (+) 315 WP_000958598.1 putative quinol monooxygenase -
  H9445_RS16460 (H9445_16460) parE 3333782..3335674 (-) 1893 WP_000195296.1 DNA topoisomerase IV subunit B -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24677.59 Da        Isoelectric Point: 6.9850

>NTDB_id=416825 H9445_RS16435 WP_001221495.1 3330106..3330765(+) (ciaR) [Escherichia coli strain ST18]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLSRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=416825 H9445_RS16435 WP_001221495.1 3330106..3330765(+) (ciaR) [Escherichia coli strain ST18]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGTCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGTT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Response regulator receiver domain (Response_reg)

(3-112)

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P52076

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

37.885

100

0.393
  ciaR Streptococcus pneumoniae D39

37.885

100

0.393
  ciaR Streptococcus pneumoniae R6

37.885

100

0.393
  ciaR Streptococcus pneumoniae TIGR4

37.885

100

0.393
  ciaR Streptococcus mutans UA159

35.426

100

0.361