Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   E3S81_RS02695 Genome accession   NZ_CP047794
Coordinates   491356..492330 (+) Length   324 a.a.
NCBI ID   WP_000697222.1    Uniprot ID   A0A2S6D0Y6
Organism   Staphylococcus aureus strain UP_551     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 486356..497330
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E3S81_RS02660 (E3S81_02660) rpmG 486816..486965 (+) 150 WP_160199095.1 50S ribosomal protein L33 -
  E3S81_RS02665 (E3S81_02665) - 487174..487713 (+) 540 WP_000164552.1 5-formyltetrahydrofolate cyclo-ligase -
  E3S81_RS02670 (E3S81_02670) - 487725..489188 (+) 1464 WP_001017867.1 rhomboid family protein -
  E3S81_RS02675 (E3S81_02675) - 489169..489372 (+) 204 WP_000087561.1 YqgQ family protein -
  E3S81_RS02680 (E3S81_02680) - 489369..490355 (+) 987 WP_000161315.1 ROK family glucokinase -
  E3S81_RS02685 (E3S81_02685) - 490355..490684 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  E3S81_RS02690 (E3S81_02690) - 490681..491304 (+) 624 WP_047213494.1 MBL fold metallo-hydrolase -
  E3S81_RS02695 (E3S81_02695) comGA 491356..492330 (+) 975 WP_000697222.1 competence type IV pilus ATPase ComGA Machinery gene
  E3S81_RS02700 (E3S81_02700) comGB 492302..493372 (+) 1071 WP_000776413.1 competence type IV pilus assembly protein ComGB Machinery gene
  E3S81_RS02705 (E3S81_02705) comGC 493386..493697 (+) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  E3S81_RS02710 (E3S81_02710) comGD 493675..494121 (+) 447 WP_072357807.1 competence type IV pilus minor pilin ComGD Machinery gene
  E3S81_RS02715 (E3S81_02715) comGE 494108..494407 (+) 300 WP_000844415.1 hypothetical protein Machinery gene
  E3S81_RS02720 (E3S81_02720) comGF 494325..494822 (+) 498 WP_001825340.1 competence type IV pilus minor pilin ComGF Machinery gene
  E3S81_RS02725 (E3S81_02725) - 494919..495065 (+) 147 WP_001789879.1 hypothetical protein -
  E3S81_RS02730 (E3S81_02730) - 495055..495579 (+) 525 WP_001015120.1 shikimate kinase -
  E3S81_RS02735 (E3S81_02735) gcvT 495738..496829 (+) 1092 WP_000093354.1 glycine cleavage system aminomethyltransferase GcvT -

Sequence

Protein


Download         Length: 324 a.a.        Molecular weight: 36874.92 Da        Isoelectric Point: 8.8508

>NTDB_id=416458 E3S81_RS02695 WP_000697222.1 491356..492330(+) (comGA) [Staphylococcus aureus strain UP_551]
MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQIGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYHF
NKIYFLRISTLPLSLGQESCVIRIVPQFFQQQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVIQASLSGHLVLTTLHATDCKGAI
LRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLVCEILSQQQLRYFFSNNHSLPSSFKNLEDKLDDMTKAGVICETTM
DKYI

Nucleotide


Download         Length: 975 bp        

>NTDB_id=416458 E3S81_RS02695 WP_000697222.1 491356..492330(+) (comGA) [Staphylococcus aureus strain UP_551]
TTGAAGATTCTATTTCAAGAAATAATTAATAAAGCGATAGAAATGAAAGCGAGTGATGTACATTTTATTCCAGTTAAAAA
TGAAGTAAGTATTAAATTTAGAATTAATGATAACTTGGAGCAGTATGAACAAATTGGGAATAGCATTTATCAAAAGTTAT
TAGTTTATATGAAGTTTCAAGCTGGGCTTGATGTTTCTACACAGCAAGTCGCACAGAGCGGTCGATATAGTTACCATTTC
AATAAAATATATTTTTTGAGAATATCAACTTTACCATTGTCACTTGGCCAAGAAAGTTGTGTAATCAGAATTGTACCTCA
ATTTTTTCAACAACAGAAATCAACTTATAAATTCAATGATTTTAAACACCTCATGAATAAGAAACAAGGATTACTATTGT
TTAGTGGACCTACTGGTTCAGGAAAGAGTACATTAATGTATCAAATGGTCTCATACGCGAATAAAGCCTTGAATTTAAAT
GTAATTTCTATAGAAGATCCTGTAGAGATGCAAATTCCTGGTATTGTCCAAATTAATGTGAATGATAAAGCTGGCATTAA
CTATGTAAATTCGTTTAAAGCTATTTTAAGATGTGATCCTGATGTTATTTTAATAGGTGAAATCAGAGATAAAGATGTTG
CCAAGTGTGTTATACAGGCTAGTTTAAGTGGTCACCTTGTTCTGACTACATTGCATGCAACTGATTGTAAAGGTGCTATT
TTAAGGCTATTAGAAATGGGCATTTCTGTACAAGAATTGATACAGGCAACTAACTTAATTATAAACCAACGACTTGTAAC
TACTATTAAGCAACAGCGACAATTAGTATGTGAAATTCTATCTCAGCAACAACTCCGATATTTCTTTTCCAATAATCATT
CATTACCATCATCATTTAAGAACTTAGAAGATAAACTTGATGATATGACAAAAGCAGGTGTCATTTGTGAAACTACAATG
GATAAATACATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2S6D0Y6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

99.691

100

0.997
  comGA Staphylococcus aureus N315

99.691

100

0.997

Multiple sequence alignment