Detailed information NTG0414887

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: GNE05_RS13405
• Genome accession: NZ_CP047644
• Coordinates: 2729572..2730186 (-)
• Length: 204 a.a.
• NCBI ID: WP_025285016.1
• Uniprot ID: -
• Organism: Bacillus sp. AM1(2019)
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2724572..2735186

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GNE05_RS13375 (GNE05_13375)	rpsT	2724875..2725141 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
GNE05_RS13380 (GNE05_13380)	holA	2725158..2726199 (-)	1042	Protein_2629	DNA polymerase III subunit delta	-
GNE05_RS13385 (GNE05_13385)	-	2726239..2726390 (-)	152	Protein_2630	hypothetical protein	-
GNE05_RS13390 (GNE05_13390)	-	2726431..2726565 (+)	135	WP_003152870.1	YqzM family protein	-
GNE05_RS13395 (GNE05_13395)	comEC	2726584..2728935 (-)	2352	WP_160182881.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
GNE05_RS13400 (GNE05_13400)	-	2728936..2729505 (-)	570	WP_003152868.1	ComE operon protein 2	-
GNE05_RS13405 (GNE05_13405)	comEA	2729572..2730186 (-)	615	WP_025285016.1	helix-hairpin-helix domain-containing protein	Machinery gene
GNE05_RS13410 (GNE05_13410)	comER	2730245..2731066 (+)	822	WP_012118027.1	late competence protein ComER	-
GNE05_RS13415 (GNE05_13415)	-	2731135..2731872 (-)	738	WP_015417856.1	class I SAM-dependent methyltransferase	-
GNE05_RS13420 (GNE05_13420)	rsfS	2731869..2732225 (-)	357	WP_007408260.1	ribosome silencing factor	-
GNE05_RS13425 (GNE05_13425)	yqeK	2732243..2732803 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
GNE05_RS13430 (GNE05_13430)	-	2732793..2733362 (-)	570	WP_160182882.1	nicotinate-nucleotide adenylyltransferase	-
GNE05_RS13435 (GNE05_13435)	yhbY	2733373..2733663 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
GNE05_RS13440 (GNE05_13440)	aroE	2733657..2734499 (-)	843	WP_160182883.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22237.37 Da
• Isoelectric Point: 5.5211

>NTDB_id=414887 GNE05_RS13405 WP_025285016.1 2729572..2730186(-) (comEA) [Bacillus sp. AM1(2019)]
MTKAMMTERLRRNLKILGACLVAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=414887 GNE05_RS13405 WP_025285016.1 2729572..2730186(-) (comEA) [Bacillus sp. AM1(2019)]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGTAGCAGGGGCGGTTTT
TATCTGCATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGACAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGCACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CGGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.639	93.627	0.549
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA	Latilactobacillus sakei subsp. sakei 23K	31.897	100	0.363
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363