Detailed information NTG0413638

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: XFGV230_RS10005
• Genome accession: NZ_CP060159
• Coordinates: 2249338..2250201 (-)
• Length: 287 a.a.
• NCBI ID: WP_004084594.1
• Uniprot ID: B2I9Q6
• Organism: Xylella fastidiosa subsp. fastidiosa strain GV230
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2244338..2255201

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
XFGV230_RS09985 (XFGV230_09985)	rimK	2244821..2245726 (+)	906	WP_004090391.1	30S ribosomal protein S6--L-glutamate ligase	-
XFGV230_RS09990 (XFGV230_09990)	-	2246429..2247106 (+)	678	WP_010894955.1	response regulator transcription factor	-
XFGV230_RS09995 (XFGV230_09995)	-	2247099..2248436 (+)	1338	WP_004090387.1	HAMP domain-containing sensor histidine kinase	-
XFGV230_RS10000 (XFGV230_10000)	coaE	2248703..2249326 (-)	624	WP_004090385.1	dephospho-CoA kinase	-
XFGV230_RS10005 (XFGV230_10005)	pilD	2249338..2250201 (-)	864	WP_004084594.1	A24 family peptidase	Machinery gene
XFGV230_RS10010 (XFGV230_10010)	pilC	2250208..2251410 (-)	1203	WP_011098309.1	type II secretion system F family protein	Machinery gene
XFGV230_RS10015 (XFGV230_10015)	pilA	2251851..2252297 (+)	447	WP_004090380.1	pilin	Machinery gene
XFGV230_RS10020 (XFGV230_10020)	-	2252543..2252770 (-)	228	WP_011098310.1	hypothetical protein	-
XFGV230_RS10025 (XFGV230_10025)	pilA/pilAI	2253606..2254046 (+)	441	WP_004090375.1	pilin	Machinery gene

Sequence

Protein

• Length: 287 a.a.
• Molecular weight: 31704.79 Da
• Isoelectric Point: 8.6709

>NTDB_id=413638 XFGV230_RS10005 WP_004084594.1 2249338..2250201(-) (pilD) [Xylella fastidiosa subsp. fastidiosa strain GV230]
MAFLDQHPGLGYPLASGLGLLLGSFLNVVILRLPRRLEWQWKRDAREILEMPDIYDPPPPGIVVEPSHDPVTGDRLKWWE
NIPVLSWLLLRGRSRYSGKSISLQYPLVELLTCLLVLASVWRFGFGWHGFGAIVFSCFLVALAGIDLRTRLLPDQLTLML
MWLGLIGSADNLFMAAKPALLGAAVGYLSLWIVAALFKLIFNKEGMGHGDFKMLGALGAWCGLKGILPIILISSLAGVVL
GSVWLVVRGHNRTTMIPFGPYLAVGGWIVFFFGDQIIHAYLELLSIG

Nucleotide

• Length: 864 bp

>NTDB_id=413638 XFGV230_RS10005 WP_004084594.1 2249338..2250201(-) (pilD) [Xylella fastidiosa subsp. fastidiosa strain GV230]
ATGGCATTTCTTGATCAGCACCCCGGACTCGGCTATCCCCTTGCGTCCGGTTTGGGGTTGTTGCTTGGTAGTTTTCTCAA
TGTGGTGATCCTGCGTTTGCCGCGCCGTTTGGAATGGCAATGGAAGCGCGATGCGCGCGAAATCCTGGAAATGCCGGACA
TTTATGATCCGCCACCGCCGGGAATTGTGGTCGAGCCTTCCCACGATCCAGTGACTGGTGATCGGTTGAAGTGGTGGGAA
AACATTCCGGTGCTTAGCTGGTTGTTGCTACGTGGACGTTCGCGTTACAGCGGCAAGTCAATCTCGTTGCAGTATCCGTT
GGTCGAATTGCTGACTTGCCTGCTGGTGCTGGCCAGTGTTTGGCGTTTTGGATTCGGTTGGCATGGTTTTGGGGCAATTG
TGTTCAGTTGTTTCCTGGTTGCGTTGGCTGGGATTGATTTGCGTACTCGGCTCTTACCTGACCAGTTAACTTTAATGCTC
ATGTGGCTGGGTTTGATTGGATCGGCGGATAACTTGTTTATGGCAGCCAAACCTGCGTTATTGGGGGCTGCAGTTGGTTA
TTTATCTCTGTGGATTGTGGCGGCGTTGTTCAAACTGATCTTTAACAAGGAAGGGATGGGCCATGGTGACTTCAAAATGC
TGGGCGCGCTTGGGGCATGGTGTGGGTTAAAGGGTATTCTCCCGATCATTTTGATTTCATCACTGGCTGGAGTGGTATTG
GGCTCTGTTTGGCTTGTTGTGCGTGGTCATAACCGTACGACGATGATTCCGTTTGGACCTTATCTGGCAGTTGGTGGATG
GATCGTGTTCTTTTTCGGCGATCAGATCATCCATGCTTATCTGGAGCTATTAAGTATCGGGTGA

Domains

Predicted by InterProScan.

• Type IV leader peptidase family (Peptidase_A24) (133-241)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (18-123)

3D structure

• AlphaFold DB: B2I9Q6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio campbellii strain DS40M4	41.754	99.303	0.415
pilD	Vibrio cholerae strain A1552	42.049	98.606	0.415
pilD	Acinetobacter nosocomialis M2	41.343	98.606	0.408
pilD	Acinetobacter baumannii D1279779	41.343	98.606	0.408
pilD	Neisseria gonorrhoeae MS11	43.284	93.38	0.404