Detailed information    

insolico Bioinformatically predicted

Overview

Name   recF   Type   Machinery gene
Locus tag   GTW28_RS00020 Genome accession   NZ_CP047485
Coordinates   1688..2800 (+) Length   370 a.a.
NCBI ID   WP_003243515.1    Uniprot ID   A0AAE2SM46
Organism   Bacillus subtilis strain BJQ0005     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1..7800
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GTW28_RS00010 (GTW28_00010) dnaN 190..1326 (+) 1137 WP_003242509.1 DNA polymerase III subunit beta -
  GTW28_RS00015 (GTW28_00015) rlbA 1457..1672 (+) 216 WP_003226810.1 ribosome maturation protein RlbA -
  GTW28_RS00020 (GTW28_00020) recF 1688..2800 (+) 1113 WP_003243515.1 DNA replication/repair protein RecF Machinery gene
  GTW28_RS00025 (GTW28_00025) remB 2818..3063 (+) 246 WP_003219266.1 extracellular matrix regulator RemB -
  GTW28_RS00030 (GTW28_00030) gyrB 3118..5034 (+) 1917 WP_003226808.1 DNA topoisomerase (ATP-hydrolyzing) subunit B -
  GTW28_RS00035 (GTW28_00035) gyrA 5245..7710 (+) 2466 WP_014475555.1 DNA topoisomerase (ATP-hydrolyzing) subunit A -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 42304.34 Da        Isoelectric Point: 7.3660

>NTDB_id=413638 GTW28_RS00020 WP_003243515.1 1688..2800(+) (recF) [Bacillus subtilis strain BJQ0005]
MYIQNLELTSYRNYDHAELQFENKVNVIIGENAQGKTNLMEAIYVLSMAKSHRTSNDKELIRWDKDYAKIEGRVMKQNGA
IPMQLVISKKGKKGKVNHIEQQKLSQYVGALNTIMFAPEDLNLVKGSPQVRRRFLDMEIGQVSPVYLHDLSLYQKILSQR
NHFLKQLQTRKQTDRTMLDVLTDQLVEVAAKVVVKRLQFTAQLEKWAQPIHAGISRGLEELTLKYHTALDVSDPLDLSKI
GDSYQEAFSKLREKEIERGVTLSGPHRDDVLFYVNGRDVQTYGSQGQQRTTALSLKLAEIDLIHEEIGEYPILLLDDVLS
ELDDYRQSHLLHTIQGRVQTFVTTTSVDGIDHETLRQAGMFRVQNGALVK

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=413638 GTW28_RS00020 WP_003243515.1 1688..2800(+) (recF) [Bacillus subtilis strain BJQ0005]
TTGTATATCCAGAACTTAGAACTGACATCTTACCGCAACTACGACCATGCTGAACTTCAATTTGAAAATAAAGTAAATGT
GATCATCGGAGAAAACGCCCAGGGGAAGACAAACCTCATGGAGGCGATCTATGTCTTGTCCATGGCGAAATCGCACCGGA
CATCAAATGACAAAGAACTTATACGGTGGGACAAAGACTATGCTAAAATAGAGGGAAGAGTGATGAAGCAAAACGGGGCA
ATCCCGATGCAGCTCGTCATCTCCAAAAAGGGTAAAAAGGGCAAGGTCAATCATATTGAACAGCAAAAGCTCAGCCAGTA
TGTCGGGGCCCTCAACACCATTATGTTCGCGCCGGAAGATTTAAATCTTGTAAAGGGAAGCCCTCAAGTGAGAAGGCGGT
TTCTTGACATGGAAATCGGACAGGTTTCTCCCGTCTACCTTCATGATCTTTCTCTTTACCAGAAAATCCTTTCCCAGCGG
AATCATTTTTTGAAACAGCTGCAAACAAGAAAACAAACTGACCGGACGATGCTCGATGTTCTGACCGATCAGCTTGTAGA
AGTTGCAGCAAAAGTCGTCGTAAAACGCCTGCAGTTTACAGCACAGCTCGAGAAATGGGCGCAGCCCATCCATGCAGGCA
TCTCAAGAGGGCTTGAAGAACTGACCCTGAAATACCATACAGCTCTTGATGTATCAGATCCCCTAGATTTGTCGAAAATA
GGAGATAGCTATCAAGAAGCGTTTTCTAAATTAAGAGAAAAAGAAATTGAGCGTGGTGTGACGCTGTCAGGGCCTCATCG
CGATGATGTTCTTTTCTATGTGAACGGACGCGATGTGCAGACGTATGGTTCTCAAGGACAGCAGCGAACGACGGCGTTGT
CCCTTAAGCTGGCGGAGATTGACCTGATCCATGAAGAAATCGGAGAATATCCCATTTTACTATTGGATGATGTACTGAGT
GAACTGGATGATTATCGCCAGTCACACTTGCTTCATACGATCCAAGGCCGTGTACAAACGTTTGTCACAACGACAAGCGT
TGATGGCATTGATCACGAAACCTTACGTCAAGCAGGAATGTTCCGTGTGCAAAATGGTGCGTTAGTGAAGTGA

Domains

Predicted by InterproScan.

RecF/RecN/SMC N terminal domain (SMC_N)

(2-350)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recF Bacillus subtilis subsp. subtilis str. 168

100

100

1

Multiple sequence alignment