Detailed information NTG0413432

Bioinformatically predicted

Overview

• Name: ciaR
• Type: Regulator
• Locus tag: FFHJCLDM_RS14565
• Genome accession: NZ_CP060121
• Coordinates: 3006430..3007089 (+)
• Length: 219 a.a.
• NCBI ID: WP_001221493.1
• Uniprot ID: Q3YXL4
• Organism: Escherichia coli BL21
• Function: repress competence development; post-transcriptional repression of CSP production (predicted from homology) Competence regulation

Genomic Context

Location: 3001430..3012089

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FFHJCLDM_RS14540 (FFHJCLDM_02948)	ygiS	3002713..3004320 (-)	1608	WP_001295629.1	ABC transporter substrate-binding protein	-
FFHJCLDM_RS14545 (FFHJCLDM_02949)	mqsA	3004453..3004848 (-)	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	-
FFHJCLDM_RS14550 (FFHJCLDM_02950)	mqsR	3004850..3005146 (-)	297	WP_000415584.1	type II toxin-antitoxin system toxin MqsR	-
FFHJCLDM_RS14555 (FFHJCLDM_02951)	ygiV	3005351..3005833 (-)	483	WP_000183505.1	GyrI-like domain-containing protein	-
FFHJCLDM_RS14560 (FFHJCLDM_02952)	ygiW	3005886..3006278 (-)	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
FFHJCLDM_RS14565 (FFHJCLDM_02953)	ciaR	3006430..3007089 (+)	660	WP_001221493.1	quorum sensing response regulator transcription factor QseB	Regulator
FFHJCLDM_RS14570 (FFHJCLDM_02954)	qseC	3007086..3008435 (+)	1350	WP_000673402.1	quorum sensing histidine kinase QseC	-
FFHJCLDM_RS14575 (FFHJCLDM_02955)	ygiZ	3008481..3008813 (-)	333	WP_000917684.1	DUF2645 family protein	-
FFHJCLDM_RS14580 (FFHJCLDM_02956)	mdaB	3009132..3009713 (+)	582	WP_000072420.1	NADPH:quinone oxidoreductase MdaB	-
FFHJCLDM_RS14585 (FFHJCLDM_02957)	ygiN	3009744..3010058 (+)	315	WP_000958598.1	putative quinol monooxygenase	-
FFHJCLDM_RS14590 (FFHJCLDM_02958)	parE	3010106..3011998 (-)	1893	WP_000195296.1	DNA topoisomerase IV subunit B	-

Sequence

Protein

• Length: 219 a.a.
• Molecular weight: 24687.63 Da
• Isoelectric Point: 6.9850

>NTDB_id=413432 FFHJCLDM_RS14565 WP_001221493.1 3006430..3007089(+) (ciaR) [Escherichia coli BL21]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide

• Length: 660 bp

>NTDB_id=413432 FFHJCLDM_RS14565 WP_001221493.1 3006430..3007089(+) (ciaR) [Escherichia coli BL21]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGTT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

• Transcriptional regulatory protein, C terminal (Trans_reg_C) (145-216)

• Response regulator receiver domain (Response_reg) (3-112)

3D structure

• AlphaFold DB: Q3YXL4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ciaR	Streptococcus pneumoniae Rx1	38.326	100	0.397
ciaR	Streptococcus pneumoniae D39	38.326	100	0.397
ciaR	Streptococcus pneumoniae R6	38.326	100	0.397
ciaR	Streptococcus pneumoniae TIGR4	38.326	100	0.397
ciaR	Streptococcus mutans UA159	35.874	100	0.365