Detailed information NTG0413201

Bioinformatically predicted

Overview

• Name: ciaR
• Type: Regulator
• Locus tag: H4P46_RS03390
• Genome accession: NZ_CP060083
• Coordinates: 707819..708478 (-)
• Length: 219 a.a.
• NCBI ID: WP_001221493.1
• Uniprot ID: Q3YXL4
• Organism: Escherichia coli strain G1/2
• Function: repress competence development; post-transcriptional repression of CSP production (predicted from homology) Competence regulation

Genomic Context

Location: 702819..713478

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
H4P46_RS03365 (H4P46_03365)	parE	702910..704802 (+)	1893	WP_000195296.1	DNA topoisomerase IV subunit B	-
H4P46_RS03370 (H4P46_03370)	ygiN	704850..705164 (-)	315	WP_000958598.1	putative quinol monooxygenase	-
H4P46_RS03375 (H4P46_03375)	mdaB	705195..705776 (-)	582	WP_000065430.1	NADPH:quinone oxidoreductase MdaB	-
H4P46_RS03380 (H4P46_03380)	ygiZ	706095..706427 (+)	333	WP_000917684.1	DUF2645 family protein	-
H4P46_RS03385 (H4P46_03385)	qseC	706473..707822 (-)	1350	WP_032231458.1	quorum sensing histidine kinase QseC	-
H4P46_RS03390 (H4P46_03390)	ciaR	707819..708478 (-)	660	WP_001221493.1	quorum sensing response regulator transcription factor QseB	Regulator
H4P46_RS03395 (H4P46_03395)	ygiW	708630..709022 (+)	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
H4P46_RS03400 (H4P46_03400)	-	709075..709176 (+)	102	Protein_677	AraC family transcriptional regulator	-
H4P46_RS03405 (H4P46_03405)	-	709183..710334 (-)	1152	WP_001254932.1	IS30-like element IS30 family transposase	-
H4P46_RS03410 (H4P46_03410)	-	710394..710780 (+)	387	Protein_679	GyrI-like domain-containing protein	-
H4P46_RS03415 (H4P46_03415)	mqsR	710985..711281 (+)	297	WP_000415584.1	type II toxin-antitoxin system toxin MqsR	-
H4P46_RS03420 (H4P46_03420)	mqsA	711283..711678 (+)	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	-
H4P46_RS03425 (H4P46_03425)	ygiS	711811..713418 (+)	1608	WP_047644522.1	ABC transporter substrate-binding protein	-

Sequence

Protein

• Length: 219 a.a.
• Molecular weight: 24687.63 Da
• Isoelectric Point: 6.9850

>NTDB_id=413201 H4P46_RS03390 WP_001221493.1 707819..708478(-) (ciaR) [Escherichia coli strain G1/2]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide

• Length: 660 bp

>NTDB_id=413201 H4P46_RS03390 WP_001221493.1 707819..708478(-) (ciaR) [Escherichia coli strain G1/2]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGTT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

• Transcriptional regulatory protein, C terminal (Trans_reg_C) (145-216)

• Response regulator receiver domain (Response_reg) (3-112)

3D structure

• AlphaFold DB: Q3YXL4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ciaR	Streptococcus pneumoniae Rx1	38.326	100	0.397
ciaR	Streptococcus pneumoniae D39	38.326	100	0.397
ciaR	Streptococcus pneumoniae R6	38.326	100	0.397
ciaR	Streptococcus pneumoniae TIGR4	38.326	100	0.397
ciaR	Streptococcus mutans UA159	35.874	100	0.365