Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   GSN03_RS00470 Genome accession   NZ_CP047366
Coordinates   91749..93125 (+) Length   458 a.a.
NCBI ID   WP_001085206.1    Uniprot ID   A0A2B8Z7F7
Organism   Bacillus nitratireducens strain BM02     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 86749..98125
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GSN03_RS00450 (GSN03_00450) ctsR 86948..87409 (+) 462 WP_001244553.1 transcriptional regulator CtsR -
  GSN03_RS00455 (GSN03_00455) - 87578..88126 (+) 549 WP_000128379.1 UvrB/UvrC motif-containing protein -
  GSN03_RS00460 (GSN03_00460) - 88131..89195 (+) 1065 WP_000050844.1 protein arginine kinase -
  GSN03_RS00465 (GSN03_00465) clpC 89218..91653 (+) 2436 WP_000971190.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  GSN03_RS00470 (GSN03_00470) radA 91749..93125 (+) 1377 WP_001085206.1 DNA repair protein RadA Machinery gene
  GSN03_RS00475 (GSN03_00475) disA 93129..94202 (+) 1074 WP_212088781.1 DNA integrity scanning diadenylate cyclase DisA -
  GSN03_RS00480 (GSN03_00480) - 94364..95473 (+) 1110 WP_000919675.1 PIN/TRAM domain-containing protein -
  GSN03_RS00485 (GSN03_00485) ispD 95490..96170 (+) 681 WP_000288303.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  GSN03_RS00490 (GSN03_00490) ispF 96288..96764 (+) 477 WP_000488381.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49870.56 Da        Isoelectric Point: 7.4250

>NTDB_id=413015 GSN03_RS00470 WP_001085206.1 91749..93125(+) (radA) [Bacillus nitratireducens strain BM02]
MAKKKTKFTCQECGYQSPKYMGKCPGCGQWNTLVEEMEPVVSSRRLNYANAIQSEVTKPRRLTEVETKSEARIETKFQEF
NRVLGGGIVDGSLVLIGGDPGIGKSTLLLQISSQLADSSYDVLYISGEESAKQIKLRADRLHVKGSNLFVVAETDLQRIA
SHIEEMNPAFVVIDSIQTIHLPEVTSAPGSVAQVRECTAELMKLAKTKGIPIFIVGHVTKEGAIAGPRMLEHMVDAVLYF
EGDRHHTYRILRAVKNRFGSTNEMGIFEMKELGLAEVLNPSEIFLEERPVGVAGSTVVASMEGTRPVLVEIQALISPTSF
GNPRRMATGIDHNRVSLIMAVLEKRTGLLLQNQDAYLKVAGGLKLDEPAIDLAVALSIASSFRDKPTAPTDAVIGEVGLT
GEIRRVSRIEQRVQEAAKLGFQRAIIPRKNLGGWTIPDGIEVVGVSNLGEALRLTLGG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=413015 GSN03_RS00470 WP_001085206.1 91749..93125(+) (radA) [Bacillus nitratireducens strain BM02]
ATGGCTAAAAAGAAAACAAAGTTTACATGTCAAGAATGTGGTTATCAGTCACCAAAATATATGGGGAAATGTCCTGGATG
TGGTCAATGGAATACGCTTGTTGAAGAGATGGAACCGGTTGTATCATCAAGACGCCTTAATTATGCCAATGCAATCCAAT
CAGAAGTAACAAAACCAAGACGCCTAACAGAAGTAGAAACAAAGTCAGAGGCGCGTATTGAAACGAAATTCCAAGAGTTT
AACCGTGTACTTGGTGGTGGGATTGTAGATGGGTCCTTAGTACTTATTGGTGGAGACCCTGGGATTGGAAAATCAACATT
GCTATTACAGATTTCATCGCAATTAGCAGATTCTTCATATGATGTACTATATATATCGGGTGAGGAGTCGGCAAAGCAGA
TTAAACTTCGTGCAGATCGTTTGCATGTAAAGGGTAGTAATCTATTTGTTGTAGCAGAGACTGATTTACAGCGAATTGCA
TCGCACATTGAAGAGATGAATCCAGCTTTTGTTGTTATTGACTCCATTCAAACGATACATTTACCTGAAGTGACGTCAGC
CCCAGGAAGTGTCGCGCAAGTACGTGAATGTACAGCGGAATTAATGAAACTTGCAAAGACGAAAGGGATTCCTATTTTTA
TAGTCGGACATGTGACAAAAGAAGGGGCAATTGCAGGACCACGTATGCTAGAACATATGGTCGATGCAGTTCTTTACTTT
GAAGGAGATCGTCACCATACATATCGTATTTTGCGAGCTGTGAAAAACCGTTTTGGTTCGACAAATGAAATGGGTATCTT
TGAAATGAAGGAGCTAGGTCTTGCAGAAGTACTAAACCCTTCTGAAATTTTCCTTGAGGAACGTCCAGTTGGAGTTGCAG
GATCCACAGTGGTTGCTTCAATGGAAGGAACAAGACCGGTTTTAGTAGAAATACAAGCATTAATCTCTCCTACTAGTTTT
GGAAATCCTCGAAGAATGGCGACGGGAATTGATCATAACCGCGTTTCGCTTATTATGGCAGTTTTAGAAAAAAGAACCGG
TTTATTGTTGCAAAATCAAGACGCGTATTTAAAAGTCGCGGGTGGTTTGAAATTAGATGAACCAGCAATCGATTTAGCCG
TTGCATTAAGTATTGCTTCGAGTTTCAGAGATAAACCTACTGCACCAACCGATGCGGTAATAGGAGAAGTAGGCCTAACT
GGAGAAATAAGAAGAGTATCAAGAATTGAACAACGTGTACAAGAAGCAGCAAAATTAGGATTTCAACGTGCTATTATTCC
TAGAAAAAATTTGGGAGGATGGACAATTCCAGATGGGATTGAGGTAGTGGGTGTTTCTAATTTAGGAGAAGCGCTTCGTT
TGACATTAGGAGGCTAG

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(363-430)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(60-222)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2B8Z7F7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

74.946

100

0.751
  radA Streptococcus pneumoniae Rx1

64.018

98.908

0.633
  radA Streptococcus pneumoniae D39

64.018

98.908

0.633
  radA Streptococcus pneumoniae R6

64.018

98.908

0.633
  radA Streptococcus pneumoniae TIGR4

64.018

98.908

0.633
  radA Streptococcus mitis NCTC 12261

64.018

98.908

0.633
  radA Streptococcus mitis SK321

64.018

98.908

0.633

Multiple sequence alignment