Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   GSY53_RS20760 Genome accession   NZ_CP047325
Coordinates   4046920..4047537 (-) Length   205 a.a.
NCBI ID   WP_004398514.1    Uniprot ID   A0AAE2SLR2
Organism   Bacillus subtilis strain GOT9     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4041920..4052537
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GSY53_RS20730 (GSY53_20730) rpsT 4042222..4042488 (+) 267 WP_003229989.1 30S ribosomal protein S20 -
  GSY53_RS20735 (GSY53_20735) holA 4042503..4043546 (-) 1044 WP_003229987.1 DNA polymerase III subunit delta -
  GSY53_RS20740 (GSY53_20740) - 4043586..4043735 (-) 150 WP_003229985.1 hypothetical protein -
  GSY53_RS20745 (GSY53_20745) yqzM 4043776..4043910 (+) 135 WP_003229983.1 YqzM family protein -
  GSY53_RS20750 (GSY53_20750) comEC 4043950..4046280 (-) 2331 WP_032677118.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  GSY53_RS20755 (GSY53_20755) comEB 4046284..4046853 (-) 570 WP_003229978.1 ComE operon protein 2 -
  GSY53_RS20760 (GSY53_20760) comEA 4046920..4047537 (-) 618 WP_004398514.1 competence protein ComEA Machinery gene
  GSY53_RS20765 (GSY53_20765) comER 4047621..4048442 (+) 822 WP_004398597.1 late competence protein ComER -
  GSY53_RS20770 (GSY53_20770) yqeM 4048508..4049251 (-) 744 WP_003229973.1 class I SAM-dependent methyltransferase -
  GSY53_RS20775 (GSY53_20775) rsfS 4049248..4049604 (-) 357 WP_003229971.1 ribosome silencing factor -
  GSY53_RS20780 (GSY53_20780) yqeK 4049622..4050182 (-) 561 WP_004399059.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  GSY53_RS20785 (GSY53_20785) nadD 4050172..4050741 (-) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  GSY53_RS20790 (GSY53_20790) yhbY 4050753..4051043 (-) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  GSY53_RS20795 (GSY53_20795) aroE 4051037..4051879 (-) 843 WP_003229967.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21769.45 Da        Isoelectric Point: 4.7220

>NTDB_id=412756 GSY53_RS20760 WP_004398514.1 4046920..4047537(-) (comEA) [Bacillus subtilis strain GOT9]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=412756 GSY53_RS20760 WP_004398514.1 4046920..4047537(-) (comEA) [Bacillus subtilis strain GOT9]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAGGAAAACGGTCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(142-203)

SLBB domain (SLBB)

(63-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

100

100

1
  comEA Staphylococcus aureus MW2

37.273

100

0.4
  comEA Staphylococcus aureus N315

36.364

100

0.39

Multiple sequence alignment