Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   GSY53_RS20285 Genome accession   NZ_CP047325
Coordinates   3962925..3963308 (-) Length   127 a.a.
NCBI ID   WP_003230168.1    Uniprot ID   -
Organism   Bacillus subtilis strain GOT9     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3957925..3968308
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GSY53_RS20245 (GSY53_20245) sinI 3958859..3959032 (+) 174 WP_003230187.1 anti-repressor SinI Regulator
  GSY53_RS20250 (GSY53_20250) sinR 3959066..3959401 (+) 336 WP_003226345.1 transcriptional regulator SinR Regulator
  GSY53_RS20255 (GSY53_20255) tasA 3959494..3960279 (-) 786 WP_003230183.1 biofilm matrix protein TasA -
  GSY53_RS20260 (GSY53_20260) sipW 3960343..3960915 (-) 573 WP_003230181.1 signal peptidase I SipW -
  GSY53_RS20265 (GSY53_20265) tapA 3960899..3961660 (-) 762 WP_003230180.1 amyloid fiber anchoring/assembly protein TapA -
  GSY53_RS20270 (GSY53_20270) yqzG 3961932..3962258 (+) 327 WP_003246051.1 YqzG/YhdC family protein -
  GSY53_RS20275 (GSY53_20275) spoIITA 3962300..3962479 (-) 180 WP_003230176.1 YqzE family protein -
  GSY53_RS20280 (GSY53_20280) comGG 3962550..3962924 (-) 375 WP_003230170.1 ComG operon protein ComGG Machinery gene
  GSY53_RS20285 (GSY53_20285) comGF 3962925..3963308 (-) 384 WP_003230168.1 ComG operon protein ComGF Machinery gene
  GSY53_RS20290 (GSY53_20290) comGE 3963334..3963681 (-) 348 WP_003230165.1 ComG operon protein 5 Machinery gene
  GSY53_RS20295 (GSY53_20295) comGD 3963665..3964096 (-) 432 WP_004398628.1 comG operon protein ComGD Machinery gene
  GSY53_RS20300 (GSY53_20300) comGC 3964086..3964382 (-) 297 WP_003230162.1 comG operon protein ComGC Machinery gene
  GSY53_RS20305 (GSY53_20305) comGB 3964396..3965433 (-) 1038 WP_009967727.1 comG operon protein ComGB Machinery gene
  GSY53_RS20310 (GSY53_20310) comGA 3965420..3966490 (-) 1071 WP_004399124.1 competence protein ComGA Machinery gene
  GSY53_RS21525 (GSY53_20315) - 3966701..3966826 (-) 126 WP_003230155.1 hypothetical protein -
  GSY53_RS20320 (GSY53_20320) corA 3966892..3967845 (-) 954 WP_032677123.1 magnesium transporter CorA -

Sequence

Protein


Download         Length: 127 a.a.        Molecular weight: 14281.37 Da        Isoelectric Point: 5.8929

>NTDB_id=412747 GSY53_RS20285 WP_003230168.1 3962925..3963308(-) (comGF) [Bacillus subtilis strain GOT9]
MLISGSLAAIIHLFLSRQQEHDGFTQQEWMISIEQMMNECKESQAVKTAEHGSVLICTNLSGQDIRFDIYHSMIRKRVDG
KGHVPILDHITAMKADIENGVVLLKIESEDQKVYQTAFPVYSYLGGG

Nucleotide


Download         Length: 384 bp        

>NTDB_id=412747 GSY53_RS20285 WP_003230168.1 3962925..3963308(-) (comGF) [Bacillus subtilis strain GOT9]
TTGCTCATATCAGGATCGTTAGCTGCGATTATCCATCTGTTTTTGTCTCGACAGCAGGAACATGACGGTTTCACACAGCA
GGAATGGATGATTTCGATAGAACAGATGATGAATGAATGCAAGGAATCACAGGCAGTTAAGACAGCCGAGCATGGGAGCG
TGTTAATCTGCACCAATCTTTCCGGACAAGACATCCGTTTTGACATTTATCATTCAATGATAAGAAAAAGAGTGGATGGC
AAAGGGCATGTTCCGATTTTAGATCATATTACTGCCATGAAAGCTGATATTGAAAATGGTGTTGTTTTGCTGAAAATTGA
GAGTGAAGACCAAAAAGTGTATCAAACTGCTTTTCCAGTCTATTCGTATTTAGGAGGGGGGTGA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(16-114)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Bacillus subtilis subsp. subtilis str. 168

100

100

1

Multiple sequence alignment