Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   H6S61_RS10545 Genome accession   NZ_CP060047
Coordinates   2113123..2114586 (-) Length   487 a.a.
NCBI ID   WP_017420351.1    Uniprot ID   -
Organism   Vibrio vulnificus strain 2497-87     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2108123..2119586
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H6S61_RS10515 (H6S61_10515) pmbA 2108188..2109530 (+) 1343 Protein_1959 metalloprotease PmbA -
  H6S61_RS10520 (H6S61_10520) mgtE 2109639..2110994 (-) 1356 WP_011078775.1 magnesium transporter -
  H6S61_RS10525 (H6S61_10525) - 2111209..2111484 (-) 276 WP_011078774.1 HPr family phosphocarrier protein -
  H6S61_RS10530 (H6S61_10530) rapZ 2111487..2112350 (-) 864 WP_017420350.1 RNase adapter RapZ -
  H6S61_RS10535 (H6S61_10535) ptsN 2112363..2112809 (-) 447 WP_011078772.1 PTS IIA-like nitrogen regulatory protein PtsN -
  H6S61_RS10540 (H6S61_10540) hpf 2112812..2113099 (-) 288 WP_011078771.1 ribosome hibernation promoting factor -
  H6S61_RS10545 (H6S61_10545) rpoN/rpoN1 2113123..2114586 (-) 1464 WP_017420351.1 RNA polymerase factor sigma-54 Machinery gene
  H6S61_RS10550 (H6S61_10550) lptB 2114640..2115365 (-) 726 WP_011078769.1 LPS export ABC transporter ATP-binding protein -
  H6S61_RS10555 (H6S61_10555) lptA 2115365..2115859 (-) 495 WP_011078768.1 lipopolysaccharide transport periplasmic protein LptA -
  H6S61_RS10560 (H6S61_10560) lptC 2115840..2116403 (-) 564 WP_185890467.1 LPS export ABC transporter periplasmic protein LptC -
  H6S61_RS10565 (H6S61_10565) kdsC 2116400..2116957 (-) 558 WP_185890469.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  H6S61_RS10570 (H6S61_10570) kdsD 2116957..2117928 (-) 972 WP_017420352.1 arabinose-5-phosphate isomerase KdsD -
  H6S61_RS10575 (H6S61_10575) - 2117950..2118915 (-) 966 WP_026050418.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 487 a.a.        Molecular weight: 54629.54 Da        Isoelectric Point: 4.3786

>NTDB_id=412744 H6S61_RS10545 WP_017420351.1 2113123..2114586(-) (rpoN/rpoN1) [Vibrio vulnificus strain 2497-87]
MKPSLQLKLGQQLAMTPQLQQAIRLLQLSTLDLQQEIQEALESNPLLEVEEHQEEAQLNGEEKAINEGSDSDDFSVESDI
PDSSDVIEKSEISSELEIDTTWDDVYSANSGNTGIALDDDMPVYQGETTETLHDYLQWQLDLTPFSDTDRMIAFAIIDAI
DDTGYLTISPEEILESLNSVDIELDEVEAVRKRIQQFDPLGVASQNLQECLLLQLATFPTETPWLAEAKMVLSHHIDALG
NRDYKLIVKEAKLKEEDLRETLKLIQQLDPRPGSKINAIEPEYVIPDVSVFKEHGKWSVAINPDSIPKLKVNQQYAQLGK
GSSADSQYIRSNLQEAKWLIKSLESRNETLLKVARCIVEHQHDFFEYGEEAMKPMVLNDVATAVEMHESTISRVTTQKFM
HTPRGIFELKYFFSSHVSTDSGGECSSTAIRALIKKLVTAENSAKPLSDSKIAALLADQGIQVARRTIAKYRESLGIAPS
SQRKRLL

Nucleotide


Download         Length: 1464 bp        

>NTDB_id=412744 H6S61_RS10545 WP_017420351.1 2113123..2114586(-) (rpoN/rpoN1) [Vibrio vulnificus strain 2497-87]
ATGAAACCTTCATTACAACTCAAGCTAGGTCAACAGTTAGCCATGACGCCTCAGCTCCAGCAGGCCATACGTTTGCTGCA
GCTGTCCACCTTGGATTTACAACAAGAAATCCAAGAAGCGCTGGAATCAAATCCGTTATTGGAAGTGGAAGAGCATCAAG
AAGAAGCGCAACTGAATGGCGAAGAGAAAGCGATCAATGAAGGTTCCGATTCGGATGATTTTTCTGTTGAGAGCGACATT
CCTGACAGCTCTGATGTGATTGAAAAATCCGAAATCAGTAGCGAACTGGAAATCGACACCACCTGGGATGACGTTTACAG
TGCCAACTCTGGCAATACAGGCATCGCACTCGACGATGACATGCCGGTCTATCAAGGTGAAACAACGGAAACACTGCACG
ACTACTTACAGTGGCAACTGGATTTAACCCCGTTTAGCGACACTGACCGCATGATCGCCTTTGCCATCATTGATGCCATC
GACGACACTGGCTACCTCACTATCAGCCCTGAAGAGATCTTAGAAAGCCTGAACAGCGTCGATATCGAACTCGACGAAGT
GGAAGCCGTACGCAAACGCATCCAACAGTTCGATCCCCTGGGTGTCGCGTCGCAAAACCTACAAGAATGCTTACTGCTGC
AACTGGCCACATTTCCGACCGAGACCCCTTGGCTAGCCGAAGCAAAAATGGTCCTGAGCCATCACATTGATGCGCTAGGC
AATCGCGATTACAAGCTGATCGTCAAAGAAGCGAAGCTCAAAGAAGAAGACCTACGTGAAACGCTCAAACTGATCCAGCA
GCTTGACCCAAGGCCTGGCAGTAAAATCAATGCCATCGAGCCCGAGTACGTGATCCCAGATGTCTCCGTATTTAAAGAGC
ATGGCAAATGGAGTGTCGCGATCAATCCAGACAGCATTCCAAAATTGAAAGTCAATCAACAATATGCGCAACTTGGCAAA
GGCAGCAGCGCCGATAGCCAGTACATTCGCAGCAATTTGCAAGAGGCGAAATGGTTAATTAAGAGCCTTGAGAGCAGAAA
CGAGACGCTGCTCAAAGTTGCACGTTGCATAGTTGAACATCAACATGATTTCTTCGAGTATGGGGAAGAAGCAATGAAAC
CTATGGTGCTAAATGATGTGGCAACCGCGGTTGAGATGCATGAATCCACCATCTCACGCGTGACAACCCAGAAGTTTATG
CACACGCCTAGAGGTATCTTTGAATTGAAATATTTCTTCTCTAGCCATGTTAGTACGGATAGTGGAGGAGAATGTTCATC
GACAGCAATTCGCGCACTCATTAAGAAGTTAGTCACTGCCGAAAACAGTGCGAAACCACTGAGTGATAGCAAAATTGCTG
CCTTACTGGCAGACCAAGGAATCCAGGTCGCACGGCGAACGATAGCTAAGTACCGCGAGTCCTTAGGCATTGCCCCATCT
AGTCAGCGAAAACGCCTACTTTAG

Domains

Predicted by InterProScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(128-315)

Sigma-54, DNA binding domain (Sigma54_DBD)

(327-485)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-49)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

43.564

100

0.452

Multiple sequence alignment