Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   GKS41_RS14770 Genome accession   NZ_CP047268
Coordinates   2993636..2994250 (-) Length   204 a.a.
NCBI ID   WP_015240245.1    Uniprot ID   -
Organism   Bacillus velezensis strain DH8043     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2988636..2999250
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GKS41_RS14740 rpsT 2988939..2989205 (+) 267 WP_003152876.1 30S ribosomal protein S20 -
  GKS41_RS14745 holA 2989222..2990263 (-) 1042 Protein_2900 DNA polymerase III subunit delta -
  GKS41_RS14750 - 2990298..2990454 (-) 157 Protein_2901 hypothetical protein -
  GKS41_RS14755 - 2990495..2990629 (+) 135 WP_003152870.1 YqzM family protein -
  GKS41_RS14760 comEC 2990648..2992999 (-) 2352 WP_159354394.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  GKS41_RS14765 - 2993000..2993569 (-) 570 WP_003152868.1 ComE operon protein 2 -
  GKS41_RS14770 comEA 2993636..2994250 (-) 615 WP_015240245.1 helix-hairpin-helix domain-containing protein Machinery gene
  GKS41_RS14775 comER 2994309..2995130 (+) 822 WP_159354395.1 late competence protein ComER -
  GKS41_RS14780 - 2995199..2995936 (-) 738 WP_015417856.1 class I SAM-dependent DNA methyltransferase -
  GKS41_RS14785 rsfS 2995933..2996289 (-) 357 WP_007408260.1 ribosome silencing factor -
  GKS41_RS14790 yqeK 2996307..2996867 (-) 561 WP_014418422.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  GKS41_RS14795 - 2996857..2997426 (-) 570 WP_017418182.1 nicotinate-nucleotide adenylyltransferase -
  GKS41_RS14800 yhbY 2997437..2997727 (-) 291 WP_003152858.1 ribosome assembly RNA-binding protein YhbY -
  GKS41_RS14805 aroE 2997721..2998563 (-) 843 WP_003152856.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 204 a.a.        Molecular weight: 22209.32 Da        Isoelectric Point: 5.5211

>NTDB_id=412027 GKS41_RS14770 WP_015240245.1 2993636..2994250(-) (comEA) [Bacillus velezensis strain DH8043]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide


Download         Length: 615 bp        

>NTDB_id=412027 GKS41_RS14770 WP_015240245.1 2993636..2994250(-) (comEA) [Bacillus velezensis strain DH8043]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGTGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CGGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-202)

SLBB domain (SLBB)

(68-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

58.854

94.118

0.554
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

35.545

100

0.368
  comEA/celA/cilE Streptococcus mitis SK321

35.071

100

0.363

Multiple sequence alignment