Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   GKS41_RS14345 Genome accession   NZ_CP047268
Coordinates   2917131..2918201 (-) Length   356 a.a.
NCBI ID   WP_159354375.1    Uniprot ID   -
Organism   Bacillus velezensis strain DH8043     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2912131..2923201
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GKS41_RS14295 sipW 2912190..2912774 (-) 585 WP_012117977.1 signal peptidase I SipW -
  GKS41_RS14300 tapA 2912746..2913417 (-) 672 WP_014418371.1 amyloid fiber anchoring/assembly protein TapA -
  GKS41_RS14305 - 2913676..2914005 (+) 330 WP_012117979.1 DUF3889 domain-containing protein -
  GKS41_RS14310 - 2914046..2914225 (-) 180 WP_003153093.1 YqzE family protein -
  GKS41_RS14315 comGG 2914282..2914659 (-) 378 WP_017418138.1 competence type IV pilus minor pilin ComGG Machinery gene
  GKS41_RS14320 comGF 2914660..2915055 (-) 396 WP_021494311.1 competence type IV pilus minor pilin ComGF -
  GKS41_RS14325 comGE 2915069..2915383 (-) 315 WP_017418140.1 competence type IV pilus minor pilin ComGE Machinery gene
  GKS41_RS14330 comGD 2915367..2915804 (-) 438 WP_159354373.1 competence type IV pilus minor pilin ComGD Machinery gene
  GKS41_RS14335 comGC 2915794..2916102 (-) 309 WP_159354374.1 competence type IV pilus major pilin ComGC Machinery gene
  GKS41_RS14340 comGB 2916107..2917144 (-) 1038 WP_053284924.1 competence type IV pilus assembly protein ComGB Machinery gene
  GKS41_RS14345 comGA 2917131..2918201 (-) 1071 WP_159354375.1 competence type IV pilus ATPase ComGA Machinery gene
  GKS41_RS14350 - 2918394..2919344 (-) 951 WP_014418379.1 magnesium transporter CorA family protein -
  GKS41_RS14355 - 2919490..2920791 (+) 1302 WP_007408318.1 hemolysin family protein -
  GKS41_RS14360 - 2920829..2921659 (-) 831 WP_039254482.1 STAS domain-containing protein -
  GKS41_RS14370 - 2921866..2922246 (-) 381 WP_003153079.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  GKS41_RS14375 - 2922483..2922728 (+) 246 WP_003153078.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40515.78 Da        Isoelectric Point: 9.3485

>NTDB_id=412023 GKS41_RS14345 WP_159354375.1 2917131..2918201(-) (comGA) [Bacillus velezensis strain DH8043]
MKNIESYSRNIINEAYTARASDIHIVPRERDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKQQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFQEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=412023 GKS41_RS14345 WP_159354375.1 2917131..2918201(-) (comGA) [Bacillus velezensis strain DH8043]
GTGAAAAATATTGAATCATACAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC
GAGAGAAAGGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGTG
TAAGGCTTATTTCTCATTTCAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGAAAGCCGCAAAACGGGTCATTGTCT
CTGCCGCTTCCGACAGAAACCGTTCATTTGAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTTATCAGGCT
TTTGCCTAAGCAGCAGATTCCCCCTCTGGATAAACTGTCCTTATTTCGGGGGGCAGGCGCCGCATTATTGTCCTTTCTGA
AGCATTCGCACGGCCTGCTTCTGTTTACCGGCCCGACCGGATCTGGAAAAACGACCACTCTTTACTCGCTTGTCAACTAT
GCAAAACGGCATTTCAACCGGCGGATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGCTGCAAGTTCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATCTTAG
GGGAAATCAGGGATGCCGAGACGGCTGAAATCGCAGTCCGCGCGGCAATGACGGGACACCTCGTTCTTTCAAGCCTGCAT
ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTCGGCGTGAACATGACAGAAATTGAACAAACCGTCATTGC
CATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCCGTGTACTGCCGGATGTACA
GGCAGACACGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTCACCCAATGCTTTCAGGAAGCAAAGGGAGAG
CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCGCTCGGCTATGTCACGACCGACAATTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

82.303

100

0.823
  pilB Glaesserella parasuis strain SC1401

38.793

97.753

0.379
  pilF Thermus thermophilus HB27

37.892

98.596

0.374

Multiple sequence alignment