Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   H4J01_RS03625 Genome accession   NZ_CP059840
Coordinates   729800..730459 (-) Length   219 a.a.
NCBI ID   WP_001221478.1    Uniprot ID   B7UIT0
Organism   Escherichia coli strain E2348/69     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 724800..735459
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H4J01_RS03600 - 725542..726489 (+) 948 WP_001296418.1 iron-siderophore ABC transporter substrate-binding protein -
  H4J01_RS03605 - 726486..727373 (+) 888 WP_000614943.1 MurR/RpiR family transcriptional regulator -
  H4J01_RS03610 ygiN 727418..727732 (-) 315 WP_000958599.1 putative quinol monooxygenase -
  H4J01_RS03615 mdaB 727763..728344 (-) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  H4J01_RS03620 qseC 728454..729803 (-) 1350 WP_000673393.1 quorum sensing histidine kinase QseC -
  H4J01_RS03625 ciaR 729800..730459 (-) 660 WP_001221478.1 quorum sensing response regulator transcription factor QseB Regulator
  H4J01_RS03630 ygiW 730611..731003 (+) 393 WP_000712664.1 OB fold stress tolerance protein YgiW -
  H4J01_RS03635 ygiV 731056..731538 (+) 483 WP_000183508.1 GyrI-like domain-containing protein -
  H4J01_RS03640 ygiS 731647..733254 (+) 1608 WP_001339995.1 ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24701.65 Da        Isoelectric Point: 6.9850

>NTDB_id=411280 H4J01_RS03625 WP_001221478.1 729800..730459(-) (ciaR) [Escherichia coli strain E2348/69]
MRILLIEDDMLIGDGIKTGLSKMGFSIDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=411280 H4J01_RS03625 WP_001221478.1 729800..730459(-) (ciaR) [Escherichia coli strain E2348/69]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCAT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTATATAGCGCGCCTTATGATGCGGTGATCCTAGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGCAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACGCTGAAACCAAAAGAATTTGCCCTGCTGGAATTG
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGCAGTGATTTTATTCGTACCGTTCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Response regulator receiver domain (Response_reg)

(3-112)

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7UIT0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365

Multiple sequence alignment