Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   GE573_RS02015 Genome accession   NZ_CP047119
Coordinates   393407..394477 (-) Length   356 a.a.
NCBI ID   WP_007408320.1    Uniprot ID   -
Organism   Bacillus velezensis strain AK-0     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 388407..399477
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GE573_RS01965 (GE573_00391) sipW 388467..389051 (-) 585 WP_015240205.1 signal peptidase I SipW -
  GE573_RS01970 (GE573_00392) tapA 389023..389694 (-) 672 WP_063094776.1 amyloid fiber anchoring/assembly protein TapA -
  GE573_RS01975 (GE573_00393) - 389953..390282 (+) 330 WP_190664424.1 DUF3889 domain-containing protein -
  GE573_RS01980 (GE573_00394) - 390322..390501 (-) 180 WP_003153093.1 YqzE family protein -
  GE573_RS01985 (GE573_00395) comGG 390558..390935 (-) 378 WP_012117980.1 competence type IV pilus minor pilin ComGG Machinery gene
  GE573_RS01990 (GE573_00396) comGF 390936..391436 (-) 501 WP_263322854.1 competence type IV pilus minor pilin ComGF -
  GE573_RS01995 (GE573_00397) comGE 391345..391659 (-) 315 WP_080130386.1 competence type IV pilus minor pilin ComGE Machinery gene
  GE573_RS02000 (GE573_00398) comGD 391643..392080 (-) 438 WP_015240210.1 competence type IV pilus minor pilin ComGD Machinery gene
  GE573_RS02005 (GE573_00399) comGC 392070..392336 (-) 267 WP_042635730.1 competence type IV pilus major pilin ComGC Machinery gene
  GE573_RS02010 (GE573_00400) comGB 392383..393420 (-) 1038 WP_080130385.1 competence type IV pilus assembly protein ComGB Machinery gene
  GE573_RS02015 (GE573_00401) comGA 393407..394477 (-) 1071 WP_007408320.1 competence type IV pilus ATPase ComGA Machinery gene
  GE573_RS02020 (GE573_00402) - 394670..395620 (-) 951 WP_007408319.1 magnesium transporter CorA family protein -
  GE573_RS02025 (GE573_00403) - 395766..397067 (+) 1302 WP_063094766.1 hemolysin family protein -
  GE573_RS02030 (GE573_00404) - 397105..397935 (-) 831 WP_015240214.1 STAS domain-containing protein -
  GE573_RS02040 (GE573_00406) - 398142..398522 (-) 381 WP_003153079.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  GE573_RS02045 (GE573_00407) - 398759..399004 (+) 246 WP_003153078.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40530.79 Da        Isoelectric Point: 9.1996

>NTDB_id=410648 GE573_RS02015 WP_007408320.1 393407..394477(-) (comGA) [Bacillus velezensis strain AK-0]
MENIETYSRNIINEAYTARASDIHIVPREKDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFQEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=410648 GE573_RS02015 WP_007408320.1 393407..394477(-) (comGA) [Bacillus velezensis strain AK-0]
GTGGAAAATATAGAAACATACAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC
GAGAGAAAAGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG
TAAGGCTAATTTCTCATTTTAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGTAAGCCGCAAAACGGGTCATTGTCT
CTGCCGCTTCCGACGGAAACCGTTCATTTGAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGCGGCAGATTCCCCCTCTGGATAAATTGTCCTTATTTCGGGGGGCGGGCGCCGCACTATTGTCCTTTCTGA
AGCATTCGCACGGTCTGCTTCTGTTTACCGGCCCCACCGGATCTGGAAAAACGACCACTCTTTACTCGCTTGTCAACTAT
GCAAAAAGGCATTTCAACCGGCGGATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGTTGCAAGTTCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGACCCCGATATGATCATCTTAG
GGGAAATCAGGGATGCCGAGACGGCTGAAATCGCAGTTCGCGCCGCCATGACGGGACACCTCGTTCTTTCAAGCCTGCAT
ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTCGGCGTGAACATGACAGAAATTGAACAAACCGTCATTGC
CATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCCGTGTACTGCCGGATGTACA
GGCAGACACGCCGTGCGGGCATTTATGAATTGCTCTACGGGAAAAACCTCACCCAATGCTTTCAGGAAGCAAAGGGAGAG
CACGCGAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATTGCGCTCGGCTATGTCACGACCGACAATTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

82.022

100

0.82
  pilB Glaesserella parasuis strain SC1401

38.793

97.753

0.379
  pilF Thermus thermophilus HB27

37.892

98.596

0.374

Multiple sequence alignment