Detailed information NTG0409339

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: GPY14_RS06100
• Genome accession: NZ_CP046918
• Coordinates: 1356340..1356954 (-)
• Length: 204 a.a.
• NCBI ID: WP_015240245.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain BA-26
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1351340..1361954

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GPY14_RS06070 (GPY14_06060)	rpsT	1351643..1351909 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
GPY14_RS06075 (GPY14_06065)	holA	1351926..1352967 (-)	1042	Protein_1227	DNA polymerase III subunit delta	-
GPY14_RS06080 (GPY14_06070)	-	1353007..1353158 (-)	152	Protein_1228	hypothetical protein	-
GPY14_RS06085 (GPY14_06075)	-	1353199..1353333 (+)	135	WP_003152870.1	YqzM family protein	-
GPY14_RS06090 (GPY14_06080)	comEC	1353352..1355703 (-)	2352	WP_039254415.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
GPY14_RS06095 (GPY14_06085)	-	1355704..1356273 (-)	570	WP_003152868.1	ComE operon protein 2	-
GPY14_RS06100 (GPY14_06090)	comEA	1356340..1356954 (-)	615	WP_015240245.1	helix-hairpin-helix domain-containing protein	Machinery gene
GPY14_RS06105 (GPY14_06095)	comER	1357013..1357834 (+)	822	WP_039254409.1	late competence protein ComER	-
GPY14_RS06110 (GPY14_06100)	-	1357903..1358640 (-)	738	WP_015417856.1	class I SAM-dependent DNA methyltransferase	-
GPY14_RS06115 (GPY14_06105)	rsfS	1358637..1358993 (-)	357	WP_007408260.1	ribosome silencing factor	-
GPY14_RS06120 (GPY14_06110)	yqeK	1359011..1359571 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
GPY14_RS06125 (GPY14_06115)	-	1359561..1360130 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
GPY14_RS06130 (GPY14_06120)	yhbY	1360141..1360431 (-)	291	WP_007408257.1	ribosome assembly RNA-binding protein YhbY	-
GPY14_RS06135 (GPY14_06125)	aroE	1360425..1361267 (-)	843	WP_039254404.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22209.32 Da
• Isoelectric Point: 5.5211

>NTDB_id=409339 GPY14_RS06100 WP_015240245.1 1356340..1356954(-) (comEA) [Bacillus velezensis strain BA-26]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=409339 GPY14_RS06100 WP_015240245.1 1356340..1356954(-) (comEA) [Bacillus velezensis strain BA-26]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGTGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363