Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   GO175_RS03345 Genome accession   NZ_CP046855
Coordinates   589079..589669 (-) Length   196 a.a.
NCBI ID   WP_020429291.1    Uniprot ID   A0AAX2LK04
Organism   Vibrio fluvialis strain F8658     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 584079..594669
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GO175_RS03325 aroB 584997..586082 (-) 1086 WP_024374091.1 3-dehydroquinate synthase -
  GO175_RS03330 aroK 586110..586628 (-) 519 WP_004728569.1 shikimate kinase AroK -
  GO175_RS03335 pilQ 586816..588552 (-) 1737 WP_024374092.1 type IV pilus secretin PilQ family protein Machinery gene
  GO175_RS03340 pilP 588577..589089 (-) 513 WP_020329446.1 pilus assembly protein PilP Machinery gene
  GO175_RS03345 pilO 589079..589669 (-) 591 WP_020429291.1 type 4a pilus biogenesis protein PilO Machinery gene
  GO175_RS03350 pilN 589662..590252 (-) 591 WP_158147935.1 PilN domain-containing protein Machinery gene
  GO175_RS03355 pilM 590242..591246 (-) 1005 WP_158147936.1 type IV pilus assembly protein PilM Machinery gene
  GO175_RS03360 - 591389..593869 (+) 2481 WP_154183958.1 PBP1A family penicillin-binding protein -

Sequence

Protein


Download         Length: 196 a.a.        Molecular weight: 22722.10 Da        Isoelectric Point: 4.7023

>NTDB_id=408936 GO175_RS03345 WP_020429291.1 589079..589669(-) (pilO) [Vibrio fluvialis strain F8658]
MANLQELELDEIAEWPLLPQLAVLLLLVLALQGAGYWLYMLPKQDALEGLKQQEQTLKSTIRIKASKVATLPKLKTQLDE
LAERYDFLLRQLPVQKELASMLASVNELGLENTLTFTRIDWGEKQNQEFLYRLPLNIELTGNYHNIGDFSEAIARLPRII
NFDDVDWQRVSQESSTLHFRVRAYTYQFKPEVSDEK

Nucleotide


Download         Length: 591 bp        

>NTDB_id=408936 GO175_RS03345 WP_020429291.1 589079..589669(-) (pilO) [Vibrio fluvialis strain F8658]
ATGGCTAACTTGCAGGAACTGGAACTGGATGAAATCGCTGAATGGCCTTTGTTGCCTCAGCTGGCCGTGTTGTTACTGCT
GGTTCTGGCTCTGCAAGGTGCGGGCTATTGGTTATATATGTTGCCCAAGCAGGATGCGCTCGAAGGTTTAAAACAGCAGG
AACAAACGCTCAAATCGACGATACGTATCAAAGCCAGCAAAGTGGCCACGTTACCCAAACTCAAAACCCAACTGGATGAA
CTGGCAGAACGGTATGACTTCCTGCTGCGTCAACTGCCGGTGCAAAAAGAGCTTGCCAGTATGCTGGCGTCGGTTAACGA
GCTGGGGCTGGAAAATACGCTGACGTTCACGCGGATTGACTGGGGGGAGAAGCAGAATCAGGAGTTCCTCTATCGTCTGC
CACTCAATATCGAACTGACCGGCAATTATCATAACATCGGCGACTTTTCCGAAGCGATTGCGCGCTTGCCACGCATCATC
AACTTTGACGATGTCGATTGGCAGCGCGTCAGTCAGGAGAGCAGCACGCTGCACTTTCGCGTCCGCGCTTACACCTATCA
GTTCAAACCGGAGGTGAGCGATGAAAAATAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(47-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio cholerae strain A1552

79.592

100

0.796
  pilO Vibrio campbellii strain DS40M4

68.947

96.939

0.668

Multiple sequence alignment