Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   GO175_RS03355 Genome accession   NZ_CP046855
Coordinates   590242..591246 (-) Length   334 a.a.
NCBI ID   WP_158147936.1    Uniprot ID   -
Organism   Vibrio fluvialis strain F8658     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 585242..596246
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GO175_RS03330 aroK 586110..586628 (-) 519 WP_004728569.1 shikimate kinase AroK -
  GO175_RS03335 pilQ 586816..588552 (-) 1737 WP_024374092.1 type IV pilus secretin PilQ family protein Machinery gene
  GO175_RS03340 pilP 588577..589089 (-) 513 WP_020329446.1 pilus assembly protein PilP Machinery gene
  GO175_RS03345 pilO 589079..589669 (-) 591 WP_020429291.1 type 4a pilus biogenesis protein PilO Machinery gene
  GO175_RS03350 pilN 589662..590252 (-) 591 WP_158147935.1 PilN domain-containing protein Machinery gene
  GO175_RS03355 pilM 590242..591246 (-) 1005 WP_158147936.1 type IV pilus assembly protein PilM Machinery gene
  GO175_RS03360 - 591389..593869 (+) 2481 WP_154183958.1 PBP1A family penicillin-binding protein -
  GO175_RS03365 oxyR 593930..594826 (-) 897 WP_020329438.1 DNA-binding transcriptional regulator OxyR -
  GO175_RS03370 - 594970..595704 (+) 735 WP_020329437.1 glutathione peroxidase -
  GO175_RS03375 - 595834..596097 (+) 264 WP_020429299.1 DUF3624 domain-containing protein -

Sequence

Protein


Download         Length: 334 a.a.        Molecular weight: 36777.10 Da        Isoelectric Point: 6.6568

>NTDB_id=408938 GO175_RS03355 WP_158147936.1 590242..591246(-) (pilM) [Vibrio fluvialis strain F8658]
MGKSLVTGVDIGHHSIKAVVLKPKGESYSLVGYQEIVVSEDIFADNHTLNYQKIVKKLKELKKGLPLFSRKVALAVPDNA
VISKVLQIDSELDAGEQEFAVYQAFSHQSPLPIEELSLDFVATGEKTLGRSSSLTYQVYATRREVIDNRLSAMSKAGFEP
LLFDMQIHGLLNIWQWVSRAQQRTNWMLVDVGFSQTSLCIDFADKTPFYKDIPLGTRQLVAATSGGNADLTSPQAHFIQD
LVERMARQIQLFLSLQGHQSLAGIWLSGGGAATEGLAQAISERLVLPCELFNPLAEFTCRASLCHHPQVDLQRFSTAAGL
ALRGLNWLENGHAA

Nucleotide


Download         Length: 1005 bp        

>NTDB_id=408938 GO175_RS03355 WP_158147936.1 590242..591246(-) (pilM) [Vibrio fluvialis strain F8658]
ATGGGTAAATCATTAGTTACAGGTGTCGATATTGGCCACCACAGTATTAAAGCGGTGGTTTTAAAACCGAAAGGCGAATC
GTATTCGCTGGTCGGTTACCAAGAGATAGTCGTATCCGAGGATATTTTCGCTGATAACCACACGCTGAATTATCAGAAAA
TTGTAAAGAAACTCAAAGAACTAAAAAAGGGATTACCGCTATTTAGTCGTAAAGTGGCGCTGGCTGTACCGGACAACGCA
GTAATAAGTAAGGTATTACAGATAGATAGCGAACTGGATGCCGGCGAACAAGAGTTCGCGGTCTATCAGGCTTTTTCCCA
TCAATCGCCGCTACCCATCGAAGAGTTGAGCCTGGATTTCGTTGCTACCGGAGAAAAAACACTGGGGCGCAGCAGTTCGT
TGACGTATCAGGTCTATGCAACCCGACGTGAAGTGATCGACAACCGTTTGTCTGCGATGAGCAAAGCGGGTTTTGAGCCG
CTGCTGTTTGATATGCAGATTCATGGCCTGCTCAATATCTGGCAATGGGTCAGCCGTGCTCAGCAACGTACCAATTGGAT
GCTGGTCGATGTCGGGTTCAGCCAAACCTCATTGTGCATCGACTTTGCCGATAAAACGCCGTTTTACAAAGACATTCCGT
TGGGAACCCGCCAACTGGTTGCGGCGACCTCCGGTGGTAACGCTGACCTTACCAGCCCGCAAGCACACTTCATTCAGGAT
TTGGTTGAGCGTATGGCGCGCCAGATCCAGCTGTTTCTTTCTCTGCAAGGCCATCAGTCGCTGGCCGGTATCTGGCTATC
CGGTGGCGGCGCGGCAACGGAAGGTTTGGCGCAGGCGATTAGCGAACGTCTGGTTCTGCCTTGCGAGCTGTTTAACCCTC
TGGCGGAGTTTACTTGCCGCGCATCGCTATGCCACCATCCTCAGGTTGATTTGCAGCGTTTCAGCACTGCGGCCGGCTTG
GCGCTGCGCGGGCTTAATTGGCTGGAGAACGGCCATGCTGCATAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-219)

Type IV pilus assembly protein PilM; (PilM_2)

(237-324)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

65.089

100

0.659
  pilM Vibrio campbellii strain DS40M4

47.246

100

0.488

Multiple sequence alignment