Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   GPX86_RS09490 Genome accession   NZ_CP046793
Coordinates   2132221..2133228 (+) Length   335 a.a.
NCBI ID   WP_158144633.1    Uniprot ID   -
Organism   Vibrio metschnikovii strain 9502-00     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2127221..2138228
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPX86_RS09475 - 2128124..2128411 (-) 288 WP_158144632.1 DUF3624 domain-containing protein -
  GPX86_RS09480 oxyR 2128646..2129545 (+) 900 WP_004394478.1 DNA-binding transcriptional regulator OxyR -
  GPX86_RS09485 - 2129599..2132079 (-) 2481 WP_110167722.1 PBP1A family penicillin-binding protein -
  GPX86_RS09490 pilM 2132221..2133228 (+) 1008 WP_158144633.1 type IV pilus assembly protein PilM Machinery gene
  GPX86_RS09495 pilN 2133218..2133811 (+) 594 WP_158144634.1 PilN domain-containing protein Machinery gene
  GPX86_RS09500 pilO 2133804..2134394 (+) 591 WP_154169997.1 type 4a pilus biogenesis protein PilO Machinery gene
  GPX86_RS09505 pilP 2134384..2134905 (+) 522 WP_040903953.1 pilus assembly protein PilP Machinery gene
  GPX86_RS09510 pilQ 2134930..2136651 (+) 1722 WP_158144635.1 type IV pilus secretin PilQ family protein Machinery gene
  GPX86_RS09515 aroK 2136838..2137356 (+) 519 WP_040903956.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 335 a.a.        Molecular weight: 37454.05 Da        Isoelectric Point: 7.4780

>NTDB_id=407877 GPX86_RS09490 WP_158144633.1 2132221..2133228(+) (pilM) [Vibrio metschnikovii strain 9502-00]
MSKSLVIGVDIGHHSIKAVALKPNGDNYSLHGCTEIVITDDIFADNDTLNYQKIVKKLKELKKSLPLFSRKVAIALPDTA
VISKVLQIDSDLDPREKEFAIYHAFSHQSPLPMEELSLDFVPLPIQAFAKNRTATYQVYATRREVVASRLSAIQQAGLEA
TLLDVQLHGLAQIWQQVSQAQQRSNWMLVDVGYTQTTLCIDFADKAPFYKDIALGTRWFELPSLAETMARSDERNQWLFN
RLFVERLTRQLQLFTSVHGQQSLAGIWLSGGGAMMDGLAETITEQLGLPCERFAPLATFVNKITQRKRLNDQPKFTTAAG
LALRGIAWLESGHVA

Nucleotide


Download         Length: 1008 bp        

>NTDB_id=407877 GPX86_RS09490 WP_158144633.1 2132221..2133228(+) (pilM) [Vibrio metschnikovii strain 9502-00]
ATGAGTAAATCATTAGTTATTGGTGTCGATATCGGTCATCACAGCATCAAAGCTGTGGCTCTTAAACCAAATGGCGACAA
TTATTCGCTACACGGTTGCACAGAAATCGTCATTACTGACGACATTTTCGCCGATAACGACACGTTGAATTATCAGAAAA
TTGTAAAGAAACTCAAAGAACTAAAAAAGAGTTTGCCGCTATTTAGTCGCAAGGTTGCGATTGCACTGCCTGATACGGCG
GTAATTAGTAAAGTATTACAAATAGATAGCGACTTAGACCCTCGTGAAAAAGAGTTTGCTATTTATCATGCTTTTTCTCA
TCAATCGCCGTTGCCGATGGAAGAACTGAGCTTAGATTTTGTTCCCTTACCGATTCAAGCGTTCGCTAAAAACCGAACTG
CGACCTATCAGGTGTATGCCACCCGAAGAGAAGTCGTCGCGAGTCGATTATCGGCGATTCAACAAGCCGGACTGGAAGCA
ACCTTACTGGATGTGCAATTACATGGTCTGGCTCAGATTTGGCAGCAGGTGAGTCAGGCTCAACAACGCTCGAATTGGAT
GTTGGTCGATGTTGGTTATACCCAAACAACGTTATGTATCGATTTTGCCGATAAAGCGCCGTTTTATAAGGATATTGCCT
TAGGTACACGCTGGTTTGAATTGCCGTCTCTGGCGGAAACGATGGCGCGCAGCGATGAACGTAACCAATGGCTATTCAAT
CGATTATTTGTCGAACGCCTAACCCGTCAACTGCAGTTATTTACTTCCGTTCATGGTCAGCAAAGCCTAGCGGGGATCTG
GTTATCTGGTGGCGGGGCGATGATGGATGGGCTGGCAGAAACCATCACCGAGCAGCTTGGATTACCCTGTGAACGGTTCG
CGCCATTAGCGACTTTTGTCAACAAAATCACTCAACGTAAGCGGTTAAACGATCAGCCTAAATTCACGACGGCTGCTGGT
CTTGCGTTGCGAGGAATAGCATGGTTGGAGAGTGGGCATGTTGCATAG

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(240-325)

Type IV pilus assembly protein PilM; (PilM_2)

(8-215)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

59.882

100

0.606
  pilM Vibrio campbellii strain DS40M4

45.672

100

0.457

Multiple sequence alignment