Detailed information    

insolico Bioinformatically predicted

Overview

Name   recG   Type   Machinery gene
Locus tag   MS8324_RS19490 Genome accession   NZ_CP058917
Coordinates   3985399..3987480 (+) Length   693 a.a.
NCBI ID   WP_001525901.1    Uniprot ID   -
Organism   Escherichia coli strain MS8324     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3980399..3992480
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MS8324_RS19470 (MS8324_3812) gmk 3981617..3982240 (+) 624 WP_001295237.1 guanylate kinase -
  MS8324_RS19475 (MS8324_3813) rpoZ 3982295..3982570 (+) 276 WP_000135058.1 DNA-directed RNA polymerase subunit omega -
  MS8324_RS19480 (MS8324_3814) spoT 3982589..3984697 (+) 2109 WP_000280473.1 bifunctional GTP diphosphokinase/guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase -
  MS8324_RS19485 (MS8324_3815) trmH 3984704..3985393 (+) 690 WP_001540097.1 tRNA (guanosine(18)-2'-O)-methyltransferase TrmH -
  MS8324_RS19490 (MS8324_3816) recG 3985399..3987480 (+) 2082 WP_001525901.1 ATP-dependent DNA helicase RecG Machinery gene
  MS8324_RS19495 (MS8324_3817) gltS 3987514..3988719 (-) 1206 WP_000468826.1 sodium/glutamate symporter -
  MS8324_RS19500 (MS8324_3818) xanP 3988999..3990390 (+) 1392 WP_001295238.1 xanthine/proton symporter XanP -
  MS8324_RS19505 (MS8324_3819) yicH 3990511..3992220 (+) 1710 WP_001774082.1 AsmA family protein -

Sequence

Protein


Download         Length: 693 a.a.        Molecular weight: 76540.50 Da        Isoelectric Point: 7.3384

>NTDB_id=407658 MS8324_RS19490 WP_001525901.1 3985399..3987480(+) (recG) [Escherichia coli strain MS8324]
MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRYEDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRM
MTCQISDGSGILTMRFFNFNAAMKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQHPAQRRLILEELLAHNLSMLALR
AGAQRFHAQPLSTNDALKNKLLAALPFKPTGAQARVVAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAVAHGKQV
ALMAPTELLAEQHANNFRNWFAPLGIEVGWLAGKQKGKARLAQQEAIASGQVQMIVGTHAIFQEQVQFNGLALVIIDEQH
RFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDELPPGRTPVTTVAIPDTRRTDIIDRVRHACM
TEGRQAYWVCTLIEESELLEAQAAEATWEELKLALPELNVGLVHGRMKPAEKQAVMTSFKQGELHLLVATTVIEVGVDVP
NASLMIIENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQKDLEIRGPGELLGTRQ
TGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIERWMPETERYSNA

Nucleotide


Download         Length: 2082 bp        

>NTDB_id=407658 MS8324_RS19490 WP_001525901.1 3985399..3987480(+) (recG) [Escherichia coli strain MS8324]
ATGAAAGGTCGCCTGTTAGATGCTGTCCCGCTCAGTTCCCTAACGGGCGTTGGCGCAGCGCTTAGTAACAAGCTGGCGAA
AATCAACCTGCATACCGTGCAGGATCTACTCTTACACCTTCCCCTGCGCTACGAAGATCGCACACATCTCTACCCCATCG
GTGAACTACTGCCGGGTGTTTATGCCACGGTGGAAGGCGAAGTGCTGAACTGCAATATCTCCTTCGGCGGTCGGCGGATG
ATGACCTGCCAGATCAGCGACGGTTCCGGCATTCTCACTATGCGCTTTTTCAACTTCAATGCGGCAATGAAAAATAGCCT
GGCGACAGGCCGCCGCGTGCTGGCCTATGGCGAAGCAAAGCGCGGCAAATATGGTGCGGAGATGATCCACCCGGAATACC
GTGTGCAGGGCGATCTCAGCACGCCAGAGTTACAGGAAACGCTCACACCGGTTTATCCAACTACGGAAGGCGTAAAGCAG
GCTACGCTGCGTAAATTAACCGACCAGGCGCTGGATCTGCTCGACACATGCGCCATTGAAGAACTCCTGCCGCCGGAACT
GTCACAAGGAATGATGACGCTACCGGAAGCGTTGCGCACTTTGCATCGCCCACCGCCGACGCTGCAGCTTAGCGATCTCG
AAACCGGGCAGCATCCGGCACAACGCCGTCTGATTCTGGAAGAGTTGCTGGCGCACAACCTCAGCATGTTGGCGTTACGT
GCCGGTGCACAACGTTTTCATGCTCAGCCGCTGAGCACCAATGACGCGCTGAAAAATAAACTCCTCGCCGCCTTACCGTT
CAAGCCAACGGGCGCACAGGCACGCGTAGTGGCGGAGATCGAGCGCGATATGGCGCTGGATGTGCCAATGATGCGCCTGG
TGCAGGGCGATGTAGGTTCCGGTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGGTTGCCCACGGCAAACAGGTG
GCCCTGATGGCACCAACCGAATTACTCGCCGAGCAGCACGCCAATAACTTCCGTAACTGGTTTGCACCGCTCGGTATCGA
AGTAGGCTGGCTCGCCGGAAAACAGAAAGGTAAAGCACGACTGGCTCAGCAAGAGGCCATCGCCAGCGGTCAGGTGCAGA
TGATTGTTGGTACGCACGCCATTTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCAT
CGTTTTGGCGTGCATCAGCGTCTGGCGTTATGGGAGAAAGGCCAGCAGCAGGGCTTCCACCCGCATCAGTTGATCATGAC
CGCCACGCCCATCCCCCGCACGCTGGCGATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAG
GCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGATATCATTGACCGCGTGCGCCACGCCTGTATG
ACTGAAGGGCGTCAGGCGTACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTACTGGAAGCGCAGGCGGCGGAAGCGAC
CTGGGAAGAGTTGAAACTGGCGCTACCAGAGTTGAATGTTGGCCTGGTACACGGACGGATGAAACCCGCCGAGAAACAGG
CTGTGATGACATCGTTTAAACAAGGCGAGCTACACCTGCTGGTTGCCACTACTGTCATTGAAGTCGGTGTTGATGTACCC
AATGCCAGTCTGATGATTATCGAAAACCCGGAGCGTCTGGGTCTGGCGCAGTTACACCAGCTTCGCGGGCGTGTAGGTCG
TGGCGCGGTGGCTTCTCACTGCGTGCTGCTCTACAAAACGCCGCTCTCCAAAACCGCGCAAATCCGCCTGCAGGTACTAC
GCGACAGCAACGATGGTTTTGTGATTGCACAGAAAGATCTGGAGATTCGCGGCCCTGGCGAATTGTTAGGTACACGTCAG
ACGGGGAATGCTGAATTTAAAGTGGCGGATTTACTGCGCGATCAGGCGATGATCCCGGAAGTTCAGCGCCTGGCGCGACA
TATTCACGAACGTTACCCACAGCAGGCAAAAGCCCTGATAGAACGCTGGATGCCAGAAACAGAACGTTACTCGAATGCGT
AA

Domains

Predicted by InterProScan.

DEAD/DEAH box helicase (DEAD)

(273-433)

Helicase conserved C-terminal domain (Helicase_C)

(481-588)

RecG wedge domain (RecG_wedge)

(15-171)

ATP-dependent DNA helicase RecG, domain 3, C-terminal (RecG_dom3_C)

(617-676)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recG Neisseria meningitidis strain C311

52.083

96.97

0.505
  recG/mmsA Streptococcus pneumoniae R6

39.074

99.711

0.39
  recG/mmsA Streptococcus pneumoniae R36A

39.074

99.711

0.39
  recG Bacillus subtilis subsp. subtilis str. 168

39.058

94.949

0.371