Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   GPY19_RS04520 Genome accession   NZ_CP046763
Coordinates   937545..938138 (-) Length   197 a.a.
NCBI ID   WP_005480482.1    Uniprot ID   Q87L64
Organism   Vibrio parahaemolyticus strain 2012AW-0353     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 932545..943138
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPY19_RS04500 aroB 933421..934521 (-) 1101 WP_025546399.1 3-dehydroquinate synthase -
  GPY19_RS04505 aroK 934553..935071 (-) 519 WP_005381319.1 shikimate kinase AroK -
  GPY19_RS04510 pilQ 935260..937008 (-) 1749 WP_005488227.1 type IV pilus secretin PilQ family protein Machinery gene
  GPY19_RS04515 pilP 937040..937555 (-) 516 WP_031778292.1 pilus assembly protein PilP Machinery gene
  GPY19_RS04520 pilO 937545..938138 (-) 594 WP_005480482.1 type 4a pilus biogenesis protein PilO Machinery gene
  GPY19_RS04525 pilN 938131..938709 (-) 579 WP_005480484.1 PilN domain-containing protein Machinery gene
  GPY19_RS04530 pilM 938693..939709 (-) 1017 WP_031778293.1 type IV pilus assembly protein PilM Machinery gene
  GPY19_RS04535 - 939852..942422 (+) 2571 WP_031778294.1 PBP1A family penicillin-binding protein -

Sequence

Protein


Download         Length: 197 a.a.        Molecular weight: 23091.63 Da        Isoelectric Point: 4.8225

>NTDB_id=407368 GPY19_RS04520 WP_005480482.1 937545..938138(-) (pilO) [Vibrio parahaemolyticus strain 2012AW-0353]
MVNWQDLEMDEIAEWPLLPQLVVICLLVLLIQGFGYWFYLKPEEARLNNLIQEEQTLKVALRIKANKVAALPQLQSQLDE
LTTRYELLLQQLPAQKELATMLASVNELGIDNKLTFTRIDWGEKQSENFLYRLPLNIELTGDFHDIGRFSQAIATLPRII
TFKDVTWQRVSQESETLHFRVRANTYQFKPEEKKDEK

Nucleotide


Download         Length: 594 bp        

>NTDB_id=407368 GPY19_RS04520 WP_005480482.1 937545..938138(-) (pilO) [Vibrio parahaemolyticus strain 2012AW-0353]
ATGGTTAATTGGCAAGATCTAGAAATGGATGAGATTGCTGAATGGCCATTACTGCCTCAGCTTGTAGTGATCTGCCTGCT
TGTTCTTCTTATCCAAGGGTTTGGTTATTGGTTTTACTTAAAGCCAGAAGAGGCTCGCTTGAACAATTTGATTCAAGAGG
AGCAAACATTAAAAGTGGCATTGCGCATAAAAGCCAACAAGGTGGCTGCATTGCCTCAATTGCAATCTCAACTGGACGAG
CTCACTACGCGTTATGAGTTGCTGTTGCAACAACTACCGGCACAAAAAGAGTTGGCCACAATGTTGGCGTCGGTAAACGA
ACTCGGTATCGACAACAAGTTAACGTTTACTCGAATCGATTGGGGAGAAAAGCAATCTGAAAACTTCCTGTATCGATTGC
CGCTAAATATCGAGCTAACAGGTGATTTCCACGATATCGGTCGTTTTTCACAGGCCATTGCCACATTGCCACGAATCATC
ACCTTTAAAGATGTGACTTGGCAGCGTGTCAGCCAAGAGAGTGAAACTTTGCACTTCCGAGTGAGAGCAAACACTTATCA
GTTCAAGCCTGAGGAAAAGAAAGATGAGAAATAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(47-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q87L64

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio campbellii strain DS40M4

94.211

96.447

0.909
  pilO Vibrio cholerae strain A1552

72.589

100

0.726

Multiple sequence alignment