Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   GPY19_RS04530 Genome accession   NZ_CP046763
Coordinates   938693..939709 (-) Length   338 a.a.
NCBI ID   WP_031778293.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain 2012AW-0353     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 933693..944709
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPY19_RS04505 aroK 934553..935071 (-) 519 WP_005381319.1 shikimate kinase AroK -
  GPY19_RS04510 pilQ 935260..937008 (-) 1749 WP_005488227.1 type IV pilus secretin PilQ family protein Machinery gene
  GPY19_RS04515 pilP 937040..937555 (-) 516 WP_031778292.1 pilus assembly protein PilP Machinery gene
  GPY19_RS04520 pilO 937545..938138 (-) 594 WP_005480482.1 type 4a pilus biogenesis protein PilO Machinery gene
  GPY19_RS04525 pilN 938131..938709 (-) 579 WP_005480484.1 PilN domain-containing protein Machinery gene
  GPY19_RS04530 pilM 938693..939709 (-) 1017 WP_031778293.1 type IV pilus assembly protein PilM Machinery gene
  GPY19_RS04535 - 939852..942422 (+) 2571 WP_031778294.1 PBP1A family penicillin-binding protein -
  GPY19_RS04540 oxyR 942478..943386 (-) 909 WP_005465160.1 DNA-binding transcriptional regulator OxyR -
  GPY19_RS04545 - 943557..944285 (+) 729 WP_015297397.1 redoxin family protein -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37820.32 Da        Isoelectric Point: 6.6637

>NTDB_id=407370 GPY19_RS04530 WP_031778293.1 938693..939709(-) (pilM) [Vibrio parahaemolyticus strain 2012AW-0353]
MDKLIVTGIDIGHNSLKAVVLKPIGDQYALLGYKEILLKEGIVAENNTINHQEIVKTLKQMKKELPFGAKRVAISVPDNS
VISKKLQIEQSLDESEIEFAVVQAFSHQSPFPVEELSLDFVRLRAEEGMRGTDSYQVFATRKDVVESRVEALQQSGLKPV
LVDVHSQSLGHIWKLAAERFPEKNKYCLLDIGSLASSFTMFTEQGELFHKEFACGTRISMGTSQEDLLSDDDQAKTEQFN
RQVVERVKRQMQLYTSINGSQNIKGIWLSGEGASTPMLAEELSHQLALECELLNPLGLFEMKVSKRKRRAADWQHFSTAA
GLAVRGIHWLGGVRALSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=407370 GPY19_RS04530 WP_031778293.1 938693..939709(-) (pilM) [Vibrio parahaemolyticus strain 2012AW-0353]
ATGGATAAGCTAATCGTTACCGGCATAGATATTGGCCATAACAGCCTCAAAGCCGTAGTACTTAAACCCATTGGCGATCA
ATACGCCTTACTGGGTTACAAAGAAATATTACTTAAGGAAGGTATTGTCGCTGAAAATAACACTATAAATCATCAAGAAA
TTGTAAAGACCCTAAAGCAGATGAAAAAAGAGCTGCCTTTTGGGGCCAAGCGAGTGGCAATTTCTGTTCCAGACAACTCA
GTTATTAGCAAGAAACTGCAAATCGAACAAAGCCTTGATGAGAGCGAAATCGAGTTTGCGGTGGTACAAGCTTTTTCTCA
TCAATCTCCTTTTCCTGTGGAAGAACTGAGTTTGGATTTTGTACGCCTTCGTGCGGAAGAAGGGATGCGTGGAACGGACA
GTTATCAAGTATTCGCTACGCGCAAAGATGTGGTTGAAAGCCGAGTTGAGGCGTTACAACAGTCTGGTTTAAAGCCTGTG
TTGGTTGACGTGCACTCTCAAAGCCTAGGTCATATCTGGAAGCTTGCCGCAGAGCGTTTTCCTGAAAAAAACAAATACTG
TTTGTTAGACATCGGCTCGCTAGCGAGTTCATTCACCATGTTTACCGAGCAAGGCGAACTGTTTCATAAGGAGTTTGCGT
GCGGTACTCGTATTTCAATGGGGACGTCACAAGAAGACTTACTCTCTGATGATGATCAAGCCAAAACGGAACAGTTCAAT
CGTCAAGTGGTTGAGCGCGTAAAACGTCAAATGCAGCTCTATACCTCGATCAATGGTTCGCAAAACATCAAAGGCATTTG
GTTATCGGGCGAGGGGGCATCGACGCCAATGTTGGCAGAAGAGCTCTCGCATCAGTTGGCATTGGAGTGCGAGTTGCTAA
ACCCACTCGGACTGTTTGAAATGAAAGTGTCGAAACGCAAGCGACGAGCGGCTGATTGGCAACATTTTTCGACAGCTGCA
GGGCTGGCGGTCCGTGGCATTCATTGGTTAGGAGGTGTGCGTGCTTTATCGCATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(237-326)

Type IV pilus assembly protein PilM; (PilM_2)

(8-215)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

84.615

100

0.846
  pilM Vibrio cholerae strain A1552

48.368

99.704

0.482

Multiple sequence alignment