Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   GPY21_RS09830 Genome accession   NZ_CP046754
Coordinates   229477..231225 (+) Length   582 a.a.
NCBI ID   WP_005488227.1    Uniprot ID   A0A7H5D3I7
Organism   Vibrio parahaemolyticus strain 2015AW-0174     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 224477..236225
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPY21_RS09810 pilM 226776..227792 (+) 1017 WP_005496568.1 type IV pilus assembly protein PilM Machinery gene
  GPY21_RS09815 pilN 227776..228354 (+) 579 WP_005496569.1 PilN domain-containing protein Machinery gene
  GPY21_RS09820 pilO 228347..228940 (+) 594 WP_005496571.1 type 4a pilus biogenesis protein PilO Machinery gene
  GPY21_RS09825 pilP 228930..229445 (+) 516 WP_005454781.1 pilus assembly protein PilP Machinery gene
  GPY21_RS09830 pilQ 229477..231225 (+) 1749 WP_005488227.1 type IV pilus secretin PilQ family protein Machinery gene
  GPY21_RS09835 aroK 231414..231932 (+) 519 WP_005381319.1 shikimate kinase AroK -
  GPY21_RS09840 aroB 231964..233064 (+) 1101 WP_005496576.1 3-dehydroquinate synthase -
  GPY21_RS09845 - 233117..234634 (+) 1518 WP_005496578.1 AAA family ATPase -
  GPY21_RS09850 - 234704..235543 (+) 840 WP_005496579.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -

Sequence

Protein


Download         Length: 582 a.a.        Molecular weight: 63751.85 Da        Isoelectric Point: 4.4353

>NTDB_id=407175 GPY21_RS09830 WP_005488227.1 229477..231225(+) (pilQ) [Vibrio parahaemolyticus strain 2015AW-0174]
MKMRQGLTRTPRMLTGLFSLWLFLLLAPLAAAEDVQSNALKSIDFRTNKSKDAVIVVELASPAAIVDVKRVQEGLSIDLM
NTSVKDEQLYLLDVKDFATVVESVEVFRDTSTTRLVAYIDEEYTHDYRLTGRYLEITVSKLKPNEKVPDKSILEKEGKLI
SINFQDIPVRNVLQLIADYNQFNLVVSDSVEGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILVAPTDELDLREKQQ
LEKQKLTEEMGELSSEIIKVNFAKASDIAEMINGEGNISMLSERGSMTIDERTNSLLIRELPENIAVIREIIESLDIPVK
QVQIEARIVTISEGNMDELGIRWGFTSINGNNTVGGSIENNLATIGLYEGGGDGEGEEGGDGVGIDDFLNVNLAATNPNA
ASIAFQVAKLGSDTLLDLELSALQQESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATSVTFKKAVLSLKVTPQ
ITPDNRLVLDLSVTQDRPGKVVKTGTGEAMAIDTQRIGTQVLVNNGETVVLGGIFQHSITNSVDKVPLLGDLPLLGALFR
RSYENVGKSELLIFVTPKVVIQ

Nucleotide


Download         Length: 1749 bp        

>NTDB_id=407175 GPY21_RS09830 WP_005488227.1 229477..231225(+) (pilQ) [Vibrio parahaemolyticus strain 2015AW-0174]
ATGAAAATGAGACAAGGATTAACACGTACACCAAGGATGCTTACTGGGCTCTTCTCCTTATGGTTGTTTTTACTTCTAGC
CCCTTTAGCAGCAGCAGAAGATGTACAGTCTAATGCGCTCAAGAGCATCGACTTTCGCACTAACAAAAGCAAAGATGCCG
TGATTGTGGTGGAGCTTGCATCCCCGGCGGCTATTGTTGATGTTAAACGTGTTCAAGAAGGATTAAGCATTGATTTAATG
AACACGTCGGTAAAAGACGAACAGCTTTACCTTCTCGACGTGAAAGATTTCGCGACGGTGGTGGAAAGTGTGGAAGTGTT
CCGCGATACCTCCACCACACGATTAGTTGCGTATATCGATGAAGAATACACGCATGACTATCGTTTGACGGGGCGTTATC
TTGAAATCACTGTCAGCAAGCTTAAACCAAATGAAAAAGTGCCAGACAAGAGCATCTTAGAAAAAGAAGGCAAGCTGATC
TCGATTAACTTCCAAGACATTCCTGTGCGTAACGTTTTGCAGCTGATTGCAGATTACAACCAGTTTAACTTGGTGGTTTC
GGACTCTGTCGAAGGCAACCTTACACTGCGTCTTGATGGTGTGCCGTGGCAGCAAGTGCTCGACATCATCTTGCAAGTGA
AAGGGTTAGATAAGCGTGTTGATGGCAACGTGATTTTGGTCGCTCCGACCGATGAGCTGGATTTGCGCGAGAAGCAGCAG
TTAGAAAAACAAAAACTGACGGAAGAAATGGGGGAGCTTTCATCAGAAATCATCAAAGTCAATTTTGCTAAAGCTTCGGA
TATTGCAGAGATGATCAATGGTGAAGGCAACATTAGTATGTTGTCAGAGCGTGGCAGTATGACCATTGATGAGCGAACCA
ATTCGCTATTAATCAGAGAATTGCCAGAGAACATCGCCGTTATTCGCGAAATCATTGAATCGCTCGATATCCCAGTCAAA
CAAGTACAAATTGAAGCCCGAATTGTCACCATCAGTGAGGGTAACATGGATGAGTTAGGTATTCGTTGGGGCTTCACATC
TATTAATGGTAATAACACCGTTGGTGGCTCTATTGAGAACAACCTAGCGACAATCGGTTTGTACGAAGGTGGTGGTGATG
GCGAAGGTGAAGAGGGTGGTGATGGTGTTGGTATCGATGATTTTCTTAATGTGAATTTAGCCGCGACCAACCCGAATGCG
GCGAGCATTGCGTTCCAAGTGGCAAAGTTAGGTTCTGATACATTATTGGACTTAGAGCTGTCTGCATTGCAGCAAGAATC
AAAAGCAGAAATCATTTCGAGCCCACGATTGATTACGACCAATAAGAAGCCCGCGTACATTGAACAAGGTACCGAAATTC
CTTATCTAGAATCTTCATCGAGTGGTGCAACGTCAGTGACCTTTAAAAAAGCGGTGTTGAGCTTGAAGGTGACACCGCAG
ATCACACCTGACAATCGTTTAGTGTTGGATCTGAGTGTGACCCAAGACCGACCTGGTAAGGTAGTGAAAACGGGCACAGG
AGAGGCAATGGCAATTGATACGCAGCGGATTGGCACCCAAGTTCTGGTGAATAATGGTGAGACGGTTGTGCTCGGTGGTA
TTTTTCAGCACAGCATTACTAACTCAGTGGATAAAGTACCATTGTTGGGCGATCTTCCGCTATTAGGCGCACTTTTCCGC
CGAAGTTACGAAAACGTTGGTAAAAGTGAGTTACTAATTTTCGTTACGCCTAAGGTCGTTATTCAGTAG

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(422-580)

Bacterial type II/III secretion system short domain (Secretin_N)

(256-321)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7H5D3I7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

91.924

100

0.919
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

76.408

97.595

0.746
  pilQ Vibrio cholerae strain A1552

76.408

97.595

0.746
  pilQ Legionella pneumophila strain ERS1305867

41.016

94.674

0.388
  pilQ Legionella pneumophila strain Lp02

41.016

94.674

0.388
  pilQ Pseudomonas aeruginosa PAK

40.468

95.533

0.387
  pilQ Acinetobacter baumannii strain A118

37.625

100

0.387
  pilQ Acinetobacter baumannii D1279779

37.625

100

0.387
  comQ Acinetobacter baylyi ADP1

38.25

100

0.383

Multiple sequence alignment