Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEC   Type   Machinery gene
Locus tag   GPW66_RS12500 Genome accession   NZ_CP046736
Coordinates   1652143..1654272 (+) Length   709 a.a.
NCBI ID   WP_242006019.1    Uniprot ID   -
Organism   Vibrio cholerae strain 3528-08     
Function   ssDNA transport through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1647143..1659272
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPW66_RS12470 - 1647492..1648067 (-) 576 WP_000999601.1 PilZ domain-containing protein -
  GPW66_RS12475 lolC 1648243..1649451 (+) 1209 WP_199244718.1 lipoprotein-releasing ABC transporter permease subunit LolC -
  GPW66_RS12480 lolD 1649444..1650130 (+) 687 WP_001061290.1 lipoprotein-releasing ABC transporter ATP-binding protein LolD -
  GPW66_RS12485 lolE 1650131..1651375 (+) 1245 WP_000493013.1 lipoprotein-releasing ABC transporter permease subunit LolE -
  GPW66_RS12495 - 1651490..1652020 (-) 531 WP_001881633.1 DUF2062 domain-containing protein -
  GPW66_RS12500 comEC 1652143..1654272 (+) 2130 WP_242006019.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  GPW66_RS12505 msbA 1654303..1656051 (+) 1749 WP_000052153.1 lipid A ABC transporter ATP-binding protein/permease MsbA -
  GPW66_RS12510 lpxK 1656054..1657061 (+) 1008 WP_199244719.1 tetraacyldisaccharide 4'-kinase -
  GPW66_RS12515 - 1657042..1657221 (+) 180 WP_000350068.1 Trm112 family protein -
  GPW66_RS12520 kdsB 1657221..1657970 (+) 750 WP_044126313.1 3-deoxy-manno-octulosonate cytidylyltransferase -

Sequence

Protein


Download         Length: 709 a.a.        Molecular weight: 79865.07 Da        Isoelectric Point: 7.3982

>NTDB_id=406840 GPW66_RS12500 WP_242006019.1 1652143..1654272(+) (comEC) [Vibrio cholerae strain 3528-08]
MVLLGYHRVGRQFLGFVAAILTIVLQGNLIRDQSNLLYQAGPDIIIKGRVDSFFTQTRYAYEGLVLIHEVNGQTLNKMTR
PRIRLSAPLLLQPNDRVEFSVTLKPIVGRLNQTGFDLEAHYMAQSVVARAVVKPDTAYQIVQESGIRSSLFFELEQLTHT
SPYQGLILALTFGERKGIDEPEWQALRNSGLIHLVAISGLHIGIAFSVGYFLGLGMMRLHAQLLWSPFVCGALLAVLYAW
LAGFTLPTQRALIMCLLNVALIMLAFPLSALKRILLTLGAVLLWSPFASLSNSFWMSFLAVAIVLYQLASQSQSQVWWKA
LLWAQVFLVCLMAPVTAYFFGGLSVTAVLYNLVFIPWFSLVIVPALFLGLLLMVVWPSVAAAYWPWVDWTFLPLDWALQF
ADVGWWVVPSKVQGVVAASVAILLHYRFMSLKACSLLLGMMGLWWWFPSLTPLWRMDVLDVGHGLAIVIEQDERAIVYDT
GSSWPGGSYVQSVIEPILQQRGLRQVDGVILSHLDNDHAGDWQGLAERWQPNWIRASQLGTEFMPCIRGESWQWQSLHFT
VLWPPQAVSRAYNQHSCVIRMTDTQSNHSVLLSGDVTAMGEWLLARDGAQLQSEVMIVPHHGSKTSSTAEFIAQVNPKLA
IASVAKDNRWNLPNPQVVARYQDQQVEWLDTGHAGQISLFFYPDQLDWFTQRSLGWQPWYRQMLRKGVE

Nucleotide


Download         Length: 2130 bp        

>NTDB_id=406840 GPW66_RS12500 WP_242006019.1 1652143..1654272(+) (comEC) [Vibrio cholerae strain 3528-08]
ATGGTTTTGCTCGGTTATCACCGAGTTGGCCGTCAATTCCTTGGCTTCGTGGCTGCCATACTAACCATTGTGCTACAGGG
CAACCTTATACGAGATCAATCCAATCTGCTCTATCAAGCAGGGCCGGATATTATCATAAAAGGCCGTGTTGACAGCTTTT
TTACGCAAACTCGTTACGCTTATGAGGGTTTGGTCCTCATTCATGAAGTGAATGGACAAACCTTAAACAAAATGACTCGC
CCTCGCATACGTTTAAGTGCCCCTTTACTGTTACAACCCAATGACCGCGTCGAATTTTCGGTAACTCTCAAGCCGATAGT
GGGTCGACTCAACCAAACCGGCTTTGATTTAGAAGCGCATTACATGGCGCAATCTGTCGTCGCACGGGCGGTCGTAAAAC
CTGACACCGCTTATCAAATTGTGCAAGAGAGTGGCATAAGGTCAAGTTTGTTTTTTGAGCTGGAACAATTAACGCATACC
AGCCCATACCAAGGATTGATCTTAGCCCTGACGTTTGGCGAGCGAAAAGGTATTGATGAGCCAGAGTGGCAAGCCTTACG
CAATAGTGGCTTAATTCATTTAGTGGCGATTTCGGGGCTACACATTGGTATCGCTTTTAGCGTGGGTTATTTTCTCGGGC
TCGGCATGATGCGTCTTCATGCTCAGTTATTGTGGTCCCCTTTTGTGTGTGGGGCTTTACTGGCGGTGCTCTACGCTTGG
CTGGCCGGATTTACTCTGCCCACTCAGCGCGCATTGATCATGTGCTTACTCAATGTGGCGTTGATCATGTTGGCTTTTCC
TCTTTCCGCGCTCAAGCGGATTCTACTCACCTTGGGCGCGGTCTTGCTTTGGTCGCCATTCGCCTCACTTTCAAACAGTT
TCTGGATGTCGTTTTTGGCGGTCGCGATTGTTCTCTACCAATTAGCCAGTCAAAGCCAGAGTCAGGTGTGGTGGAAAGCT
CTGCTTTGGGCGCAGGTGTTCCTCGTCTGTTTAATGGCACCGGTCACGGCCTATTTTTTCGGTGGCTTAAGCGTAACGGC
AGTTCTGTACAATTTGGTGTTTATTCCTTGGTTTTCGTTGGTGATTGTCCCAGCTTTGTTTTTGGGCCTACTACTCATGG
TGGTATGGCCTAGTGTGGCCGCCGCTTACTGGCCTTGGGTGGATTGGACGTTTTTACCGCTCGATTGGGCTTTGCAGTTT
GCCGATGTAGGCTGGTGGGTGGTCCCCAGCAAAGTACAAGGTGTAGTCGCAGCGAGTGTGGCCATCCTCTTGCATTATCG
ATTTATGAGCCTAAAAGCCTGCAGCTTATTATTGGGTATGATGGGCTTATGGTGGTGGTTTCCCTCTCTCACTCCACTTT
GGCGAATGGATGTGCTGGATGTTGGACATGGCTTGGCGATTGTGATTGAGCAAGATGAGCGAGCAATTGTCTACGATACA
GGCAGCAGTTGGCCGGGAGGCAGCTATGTGCAAAGCGTGATTGAGCCTATTCTCCAACAGCGAGGGCTACGCCAAGTCGA
TGGAGTGATTTTAAGTCATCTTGATAATGATCATGCGGGTGATTGGCAAGGTTTAGCTGAGCGCTGGCAACCTAATTGGA
TTCGTGCCAGCCAACTCGGGACAGAGTTTATGCCTTGTATCCGTGGTGAAAGCTGGCAGTGGCAATCTCTCCATTTTACG
GTGTTATGGCCACCACAAGCGGTTAGCCGAGCGTACAACCAGCATTCGTGTGTGATTCGTATGACTGATACTCAGTCTAA
CCATTCTGTACTGCTCTCCGGGGATGTCACAGCCATGGGGGAGTGGCTGCTTGCTCGAGACGGAGCGCAACTGCAAAGTG
AGGTGATGATCGTGCCGCATCACGGCAGTAAAACGTCGTCCACCGCAGAGTTTATTGCCCAAGTGAATCCCAAACTTGCG
ATTGCTTCTGTGGCGAAAGATAACCGCTGGAATTTGCCTAATCCGCAAGTCGTGGCACGTTATCAAGATCAGCAAGTTGA
GTGGCTAGATACTGGACACGCTGGGCAAATTAGCCTCTTTTTCTATCCAGATCAGCTGGATTGGTTTACCCAGCGTAGCC
TTGGCTGGCAGCCTTGGTATAGGCAGATGCTGCGTAAAGGAGTAGAATGA

Domains

Predicted by InterproScan.

Competence protein (Competence)

(170-424)

Domain of unknown function (DUF4131) (DUF4131)

(12-135)

Metallo-beta-lactamase superfamily (Lactamase_B)

(461-641)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEC Vibrio cholerae strain A1552

98.449

100

0.984
  comEC Vibrio parahaemolyticus RIMD 2210633

41.573

100

0.417
  comEC Vibrio campbellii strain DS40M4

41.018

99.718

0.409

Multiple sequence alignment