Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   GPW66_RS08830 Genome accession   NZ_CP046736
Coordinates   866977..867993 (+) Length   338 a.a.
NCBI ID   WP_158106336.1    Uniprot ID   -
Organism   Vibrio cholerae strain 3528-08     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 861977..872993
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPW66_RS08815 - 862517..863248 (-) 732 WP_000495226.1 glutathione peroxidase -
  GPW66_RS08820 oxyR 863388..864281 (+) 894 WP_001025909.1 DNA-binding transcriptional regulator OxyR -
  GPW66_RS08825 - 864357..866834 (-) 2478 WP_000668738.1 penicillin-binding protein 1A -
  GPW66_RS08830 pilM 866977..867993 (+) 1017 WP_158106336.1 type IV pilus assembly protein PilM Machinery gene
  GPW66_RS08835 pilN 867983..868567 (+) 585 WP_142541178.1 PilN domain-containing protein Machinery gene
  GPW66_RS08840 pilO 868560..869150 (+) 591 WP_000157690.1 type 4a pilus biogenesis protein PilO Machinery gene
  GPW66_RS08845 pilP 869140..869658 (+) 519 WP_158106600.1 pilus assembly protein PilP Machinery gene
  GPW66_RS08850 pilQ 869684..871399 (+) 1716 WP_199244755.1 type IV pilus secretin PilQ family protein Machinery gene
  GPW66_RS08855 aroK 871586..872110 (+) 525 WP_000818616.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37719.52 Da        Isoelectric Point: 7.2846

>NTDB_id=406826 GPW66_RS08830 WP_158106336.1 866977..867993(+) (pilM) [Vibrio cholerae strain 3528-08]
MGKSLVTGIDIGHHSIKAAVLKPMGDTYALVGYEELLVTADIFTDNHTLDYQKIVKKLKELKKGLPLFSHKVAIAIPDSA
VISKVLQIDSDLEQRELEFAVYQAFSHQSPFPVEELSLDFVKVTEKSLARSCTTTFQVYATKKDVVDSRLHAVKKAGFEP
VLMDVQVHSLLHLWQLASRAYRRPDWMLIDIGYTQSSMCLDFPEKMPFYKDVPLGTRQLESEPSAVNSLSHPLLQDPTQR
FIQELVDKIARQIQLFTSVHGTQSLGGLWLSGGGATLSGLEDALYQRLSLPCEVLNPFSLFKNNVAKRKRPLVDGQRFST
AAGLALRGLAWLESEHVA

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=406826 GPW66_RS08830 WP_158106336.1 866977..867993(+) (pilM) [Vibrio cholerae strain 3528-08]
ATGGGTAAATCATTAGTTACGGGTATTGATATCGGCCACCACAGCATTAAAGCTGCGGTGCTCAAACCTATGGGCGACAC
TTATGCGCTTGTGGGGTATGAGGAGTTGCTCGTTACCGCTGATATTTTCACCGATAATCACACGCTCGATTATCAGAAAA
TTGTAAAGAAACTAAAAGAACTAAAAAAGGGTTTGCCTCTATTTAGTCATAAAGTCGCGATAGCAATCCCGGATAGCGCC
GTGATTAGCAAAGTATTACAAATAGATAGCGATCTTGAGCAGCGCGAGCTGGAATTTGCTGTCTATCAAGCCTTCTCGCA
TCAATCTCCATTCCCCGTTGAAGAGCTGAGCCTCGATTTCGTCAAAGTGACGGAAAAAAGCCTCGCCCGCAGCTGCACCA
CAACCTTCCAAGTGTATGCCACCAAAAAAGACGTGGTCGACAGTCGTTTGCATGCCGTTAAAAAAGCGGGTTTTGAACCT
GTGTTGATGGATGTGCAAGTACATAGTTTGCTGCATTTATGGCAACTCGCCAGCCGTGCTTATCGACGCCCAGACTGGAT
GCTGATTGATATTGGTTATACCCAAAGTTCAATGTGCCTCGATTTTCCGGAGAAAATGCCCTTTTACAAAGATGTACCAT
TAGGAACTCGTCAATTGGAATCGGAACCCAGTGCGGTGAACTCATTGAGTCATCCACTGCTGCAAGATCCTACACAGCGA
TTTATTCAAGAATTGGTCGACAAAATTGCGCGGCAAATCCAGCTTTTTACTTCGGTGCACGGGACTCAAAGTCTTGGCGG
TTTATGGCTATCGGGTGGCGGTGCCACGCTTTCTGGTTTAGAGGACGCGCTTTATCAACGCTTGTCATTGCCTTGCGAAG
TGCTCAATCCGTTTTCACTGTTCAAAAACAATGTGGCAAAACGTAAACGCCCACTGGTTGATGGCCAGCGTTTTAGTACC
GCCGCCGGTCTTGCATTGCGAGGCTTAGCTTGGTTGGAGAGTGAGCATGTTGCATAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(240-328)

Type IV pilus assembly protein PilM; (PilM_2)

(8-221)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

98.521

100

0.985
  pilM Vibrio campbellii strain DS40M4

48.521

100

0.485

Multiple sequence alignment