Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   GPW66_RS08850 Genome accession   NZ_CP046736
Coordinates   869684..871399 (+) Length   571 a.a.
NCBI ID   WP_199244755.1    Uniprot ID   -
Organism   Vibrio cholerae strain 3528-08     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 864684..876399
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPW66_RS08830 pilM 866977..867993 (+) 1017 WP_158106336.1 type IV pilus assembly protein PilM Machinery gene
  GPW66_RS08835 pilN 867983..868567 (+) 585 WP_142541178.1 PilN domain-containing protein Machinery gene
  GPW66_RS08840 pilO 868560..869150 (+) 591 WP_000157690.1 type 4a pilus biogenesis protein PilO Machinery gene
  GPW66_RS08845 pilP 869140..869658 (+) 519 WP_158106600.1 pilus assembly protein PilP Machinery gene
  GPW66_RS08850 pilQ 869684..871399 (+) 1716 WP_199244755.1 type IV pilus secretin PilQ family protein Machinery gene
  GPW66_RS08855 aroK 871586..872110 (+) 525 WP_000818616.1 shikimate kinase AroK -
  GPW66_RS08860 aroB 872138..873223 (+) 1086 WP_071181328.1 3-dehydroquinate synthase -
  GPW66_RS08865 - 873278..874753 (+) 1476 WP_044127028.1 AAA family ATPase -
  GPW66_RS08870 - 874823..875656 (+) 834 WP_000744680.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62368.50 Da        Isoelectric Point: 4.5760

>NTDB_id=406830 GPW66_RS08850 WP_199244755.1 869684..871399(+) (pilQ) [Vibrio cholerae strain 3528-08]
MKNGLKTYVAQTWLTLWVGLALCASSMVFSAESATANQLENIDFRVNKEKAAVLIVELASPSAVVDVQKVQEGLSIELLK
TDVADDKLYLLDVKDFSTPVESVEVFRKEPSTQLVVTVDGEFQHDYTLKGKYLEVVISKLKADEKPKPKSVLEKEGKLIS
INFQDIPVRNVLQLIADYNGFNLVVSDSVVGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILIAPKEELDLREKQAL
EKARLAEELGDLKSEIIKINFAKASDIAAMIGGEGNVNMLSERGSISIDERTNSLLIRELPDNIVVIREIIESLDIPVKQ
VQIEARIVTVKEGNLDELGVRWGVMSTNGSHSVGGTIESNLWQQGLLADDEFPVDEFLNVNLASTSANASSIAFQVAKLG
SGTLLDLELSALQNESKAEIISSPRLITTNKQPAYIEQGTEIPYLESSSSGASTVAFKKAVLSLKVTPQITPDNRLVLDL
SVTQDRRGETVKTGTGEAVSIDTQRIGTQVLVNNGETVVLGGIFQHSINNSVDKVPLLGDLPVLGALFRRTYEQMGKSEL
LIFVTPKVVIQ

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=406830 GPW66_RS08850 WP_199244755.1 869684..871399(+) (pilQ) [Vibrio cholerae strain 3528-08]
ATGAAAAATGGATTGAAAACTTATGTCGCACAGACTTGGCTGACCTTGTGGGTTGGTTTGGCACTTTGTGCTTCTTCCAT
GGTGTTTAGTGCTGAATCCGCTACCGCCAACCAGTTGGAAAACATCGACTTTCGGGTCAACAAAGAGAAGGCGGCGGTCT
TAATTGTCGAGTTAGCTTCGCCATCGGCGGTCGTGGATGTGCAAAAAGTCCAAGAAGGGTTGAGCATTGAACTCCTGAAA
ACCGATGTGGCGGATGACAAGCTGTATCTACTTGATGTGAAAGATTTTTCGACCCCTGTTGAGAGCGTGGAAGTATTCCG
TAAAGAGCCAAGCACACAACTGGTTGTGACTGTCGATGGGGAGTTTCAGCACGATTATACCTTGAAAGGAAAATACCTTG
AGGTCGTGATCAGTAAACTCAAAGCTGATGAAAAGCCTAAGCCCAAGAGTGTCTTGGAAAAAGAAGGCAAGCTTATCTCA
ATTAACTTCCAAGATATTCCGGTGCGTAACGTACTGCAGCTGATTGCCGATTATAACGGTTTCAACTTAGTGGTTTCCGA
TTCGGTGGTGGGTAACCTGACTTTACGCTTGGATGGCGTGCCTTGGCAGCAAGTGTTGGACATTATTCTGCAAGTCAAAG
GGCTCGATAAACGGGTCGATGGCAATGTGATTTTGATTGCGCCGAAAGAGGAACTCGACTTGCGCGAGAAACAAGCGCTC
GAAAAAGCCCGTTTAGCAGAAGAGTTGGGCGATTTAAAATCAGAAATCATCAAGATTAACTTTGCCAAAGCGTCTGACAT
TGCGGCGATGATCGGCGGTGAAGGCAACGTTAACATGTTGTCTGAACGAGGTTCGATCAGCATCGACGAACGAACTAACT
CGCTATTGATCCGAGAGCTGCCCGATAACATTGTTGTGATCCGCGAGATCATTGAATCGCTGGATATTCCGGTCAAACAA
GTTCAAATCGAAGCGCGGATTGTCACCGTCAAAGAAGGTAACTTGGATGAACTTGGTGTGCGTTGGGGTGTGATGTCGAC
CAATGGTAGTCACTCCGTGGGTGGAACCATTGAAAGTAACTTATGGCAGCAAGGTTTATTGGCAGATGATGAATTTCCGG
TGGATGAATTCTTAAATGTGAATTTGGCTTCCACCTCTGCCAATGCGTCTTCGATTGCTTTCCAAGTCGCTAAATTGGGC
TCGGGTACGTTGTTGGATTTGGAGCTTTCAGCATTACAAAATGAATCGAAAGCCGAAATCATCTCTAGCCCTCGCTTGAT
TACCACCAATAAACAGCCTGCCTATATTGAGCAAGGTACTGAGATCCCTTATCTGGAATCGTCTTCCAGTGGTGCCTCTA
CGGTCGCGTTTAAAAAAGCGGTATTGAGCTTGAAGGTGACACCACAAATCACCCCAGATAACCGCTTGGTGCTCGACTTG
AGTGTGACCCAAGACCGACGCGGTGAAACGGTGAAAACCGGTACGGGTGAAGCGGTTTCTATTGATACTCAGCGCATTGG
CACTCAAGTGTTGGTGAATAACGGAGAAACTGTGGTGTTGGGTGGCATTTTCCAACACAGCATCAATAATTCGGTAGATA
AGGTGCCACTGCTGGGGGACTTGCCTGTTTTGGGCGCGTTATTCAGACGAACCTATGAGCAAATGGGCAAAAGTGAACTG
CTGATTTTTGTCACGCCGAAAGTGGTCATTCAATAA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(255-320)

Bacterial type II and III secretion system protein (Secretin)

(411-569)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio cholerae strain A1552

99.299

100

0.993
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

99.299

100

0.993
  pilQ Vibrio campbellii strain DS40M4

78.545

96.322

0.757
  pilQ Legionella pneumophila strain ERS1305867

42.963

94.571

0.406
  pilQ Legionella pneumophila strain Lp02

42.963

94.571

0.406
  comQ Acinetobacter baylyi ADP1

39.161

100

0.392
  pilQ Acinetobacter baumannii D1279779

37.819

100

0.389
  pilQ Acinetobacter baumannii strain A118

37.819

100

0.389
  pilQ Pseudomonas aeruginosa PAK

39.312

96.673

0.38

Multiple sequence alignment