Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   GO600_RS02610 Genome accession   NZ_CP046617
Coordinates   449613..450746 (-) Length   377 a.a.
NCBI ID   WP_028493408.1    Uniprot ID   -
Organism   Thermus antranikianii strain JCM 19900     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 444613..455746
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GO600_RS02590 (GO600_02585) pilQ 445137..447401 (-) 2265 WP_418954023.1 secretin N-terminal domain-containing protein Machinery gene
  GO600_RS02595 pilW 447413..447589 (-) 177 WP_245563304.1 hypothetical protein Machinery gene
  GO600_RS02600 (GO600_02595) pilO 448381..449007 (-) 627 WP_028493410.1 type 4a pilus biogenesis protein PilO Machinery gene
  GO600_RS02605 (GO600_02600) pilN 448997..449620 (-) 624 WP_028493409.1 competence protein Machinery gene
  GO600_RS02610 (GO600_02605) pilM 449613..450746 (-) 1134 WP_028493408.1 type IV pilus assembly protein PilM Machinery gene
  GO600_RS02615 (GO600_02610) - 450938..451942 (-) 1005 WP_028493407.1 homoisocitrate dehydrogenase -
  GO600_RS02620 (GO600_02615) - 452012..452506 (-) 495 WP_028493406.1 DUF5317 domain-containing protein -
  GO600_RS02625 (GO600_02620) - 452557..453963 (-) 1407 WP_028493405.1 HD-GYP domain-containing protein -
  GO600_RS02640 (GO600_02640) - 454864..455229 (+) 366 WP_028493404.1 hypothetical protein -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 41146.68 Da        Isoelectric Point: 4.4462

>NTDB_id=406060 GO600_RS02610 WP_028493408.1 449613..450746(-) (pilM) [Thermus antranikianii strain JCM 19900]
MLSGFSKLFKPRVEALGLEIGAANLKLVELSGNPPTLRALATRPTPPGMLVEGVIAEPQALAQELKELLAEARTKKRYVV
TAVPNPSVILRTLQVPKMPPKEMEEAVRWEAERYIPFPIDEVVLDFAPLDPLAEAAEGEQVEVMVGAARQEAVASLLEAL
RGAGLTPIILDVKPFAGLYPLEAQLSSDPEGVSVAVEIGAESTSLVLLKGNRPLAVRILALSGKDFTEAIGKSFGLDFLT
AEEVKRTYGLATIPTEDEELLLDFDAERERYSPAKIYDAIRPVLVELTQEIRRSLEFFRVQLGDVQPEVGYLYGGGSRLR
GLSTLLTDTLGVNFTVPDPWQGIQVDPKRFDLEKIKEMGPEFLVPVGLALRGVMPLD

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=406060 GO600_RS02610 WP_028493408.1 449613..450746(-) (pilM) [Thermus antranikianii strain JCM 19900]
GTGCTTTCGGGTTTTAGCAAGCTATTCAAACCCCGCGTGGAGGCGCTGGGCCTGGAGATCGGCGCCGCCAACCTAAAACT
GGTGGAACTCTCCGGAAACCCTCCCACCCTCAGGGCCTTGGCCACCCGGCCCACTCCCCCGGGAATGCTGGTGGAAGGGG
TGATCGCCGAACCCCAGGCCCTGGCCCAGGAACTCAAGGAACTCCTTGCCGAAGCCCGGACCAAAAAGCGCTACGTGGTC
ACCGCCGTACCCAATCCCAGCGTTATCCTGCGCACCCTTCAGGTGCCCAAGATGCCCCCCAAGGAGATGGAGGAGGCGGT
TCGCTGGGAAGCAGAGCGGTATATCCCCTTCCCCATTGACGAGGTGGTCCTGGACTTCGCTCCCTTGGATCCGTTGGCCG
AGGCCGCCGAAGGGGAACAGGTGGAGGTGATGGTGGGAGCAGCCCGGCAAGAAGCGGTGGCCTCCCTCCTAGAAGCCCTG
CGGGGGGCAGGCCTGACCCCCATCATCCTGGATGTGAAACCCTTTGCCGGGCTTTATCCCCTGGAGGCCCAGCTCAGTAG
CGACCCGGAGGGGGTTTCCGTGGCCGTGGAAATCGGGGCGGAAAGCACCAGCCTGGTCCTCCTTAAGGGAAACCGCCCCT
TGGCGGTGCGGATCCTCGCCCTCTCTGGCAAGGACTTCACCGAAGCCATTGGGAAAAGCTTTGGCCTGGACTTCCTCACC
GCCGAGGAGGTGAAGCGCACCTACGGCCTCGCCACCATCCCCACCGAGGACGAGGAGCTCCTTCTGGATTTTGACGCCGA
GAGGGAGCGGTACAGTCCCGCCAAAATCTACGACGCCATCCGGCCTGTCCTGGTGGAACTCACCCAGGAAATCCGCAGGA
GCCTGGAGTTTTTCCGGGTACAACTGGGGGACGTACAGCCAGAGGTAGGGTACCTTTATGGCGGGGGAAGCCGGCTTAGG
GGCCTGTCCACCCTGCTTACCGATACCCTGGGGGTCAACTTCACCGTCCCCGATCCCTGGCAGGGCATTCAGGTGGATCC
CAAACGCTTTGACCTGGAAAAGATCAAGGAGATGGGACCGGAGTTCCTGGTGCCCGTGGGCTTGGCCTTACGGGGGGTGA
TGCCCCTTGATTAG

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(17-372)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Thermus thermophilus HB27

75.597

100

0.756

Multiple sequence alignment