Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilS   Type   Regulator
Locus tag   GO602_RS24370 Genome accession   NZ_CP046602
Coordinates   5214864..5216456 (+) Length   530 a.a.
NCBI ID   WP_024914652.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain CMC-115     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 5209864..5221456
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GO602_RS24350 (GO602_24325) pgeF 5211614..5212342 (-) 729 WP_024914656.1 peptidoglycan editing factor PgeF -
  GO602_RS24355 (GO602_24330) rluD 5212339..5213301 (-) 963 WP_024914655.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  GO602_RS24360 (GO602_24335) - 5213447..5214472 (+) 1026 WP_024914654.1 outer membrane protein assembly factor BamD -
  GO602_RS24365 (GO602_24340) - 5214629..5214874 (+) 246 WP_024914653.1 PP0621 family protein -
  GO602_RS24370 (GO602_24345) pilS 5214864..5216456 (+) 1593 WP_024914652.1 two-component system sensor histidine kinase PilS Regulator
  GO602_RS24375 (GO602_24350) pilR 5216471..5217808 (+) 1338 WP_024914651.1 two-component system response regulator PilR Regulator
  GO602_RS24380 (GO602_24355) thiO 5217853..5218947 (-) 1095 WP_024914650.1 glycine oxidase ThiO -
  GO602_RS24385 (GO602_24360) - 5219082..5219591 (+) 510 WP_024914649.1 Tfp pilus assembly protein FimT/FimU -
  GO602_RS24390 (GO602_24365) - 5219697..5220203 (+) 507 WP_003102598.1 Tfp pilus assembly protein FimT/FimU -
  GO602_RS24395 (GO602_24370) pilV 5220194..5220751 (+) 558 WP_058141282.1 type 4a pilus minor pilin PilV Machinery gene

Sequence

Protein


Download         Length: 530 a.a.        Molecular weight: 59066.17 Da        Isoelectric Point: 6.5043

>NTDB_id=405974 GO602_RS24370 WP_024914652.1 5214864..5216456(+) (pilS) [Pseudomonas aeruginosa strain CMC-115]
MRAERLRLSEEQGQRILRLYHLYRLTIGLVLVLLISSELEEQVLKLVHPELFHVGSWCYLVFNILVALFLPPSRQLLPIF
ILALTDVLMLCGLFYAGGGVPSGIGSLLVVAVAIANILLRGRIGLVIAAAASLGLLYLTFFLSLNSPDATNHYVQAGGLG
TLCFAAALVIQALVRRQEQTETLAEERAETVANLEELNALILQRMRTGILVVDSRQAILLANQAALGLLRQDDVQGASLS
HHSPMLLHCMKQWRLNPSLRPPTLKVVPDGPTVQPSFIGLNREDDQHVLIFLEDISQIAQQAQQMKLAGLGRLTAGIAHE
IRNPLGAISHAAQLLQESEELDAPDRRLTQIIQDQSKRMNLVIENVLQLSRRRQAEPQQLDLKEWLRRFVDEYPGRLRND
SELHLQLGDGDIQTRMDPHQLNQVLSNLVQNGLRYSAQAHGRGQVWLSLARDPESDLPVLEVIDDGPGVATDKLNNLFEP
FFTTESKGTGLGLYLSRELCESNQARIDYRNREEGGGCFRITFAHPRKLS

Nucleotide


Download         Length: 1593 bp        

>NTDB_id=405974 GO602_RS24370 WP_024914652.1 5214864..5216456(+) (pilS) [Pseudomonas aeruginosa strain CMC-115]
GTGCGCGCTGAGCGGCTACGGCTGAGCGAGGAGCAGGGGCAACGCATCCTCCGCCTGTACCACCTGTACCGCCTGACCAT
CGGCCTGGTGCTGGTCCTGCTGATCTCCAGCGAGCTGGAAGAGCAGGTCCTCAAGCTCGTCCACCCTGAACTGTTCCATG
TCGGCAGCTGGTGCTACCTGGTCTTCAACATCCTCGTCGCGCTGTTCCTGCCTCCGTCACGGCAACTCCTGCCGATCTTC
ATCCTCGCCCTCACCGACGTGTTGATGCTTTGCGGCCTGTTCTACGCAGGTGGCGGCGTGCCCAGCGGCATCGGCAGCCT
GCTGGTGGTGGCGGTGGCCATTGCCAACATCCTGCTGCGCGGGCGCATCGGCCTGGTCATCGCGGCGGCGGCCAGCCTCG
GCCTGCTCTACCTGACCTTCTTCCTCAGCCTGAACAGTCCCGACGCCACCAACCACTACGTCCAGGCCGGCGGCCTCGGT
ACCCTGTGCTTCGCCGCCGCGCTGGTGATCCAGGCCCTGGTACGGCGCCAGGAGCAGACCGAAACGCTGGCCGAAGAACG
CGCCGAGACGGTCGCCAACCTGGAGGAGCTCAACGCATTGATCCTGCAGCGCATGCGCACCGGCATCCTCGTGGTCGACA
GCCGCCAGGCCATCCTCCTCGCCAACCAGGCCGCCCTCGGCCTGCTCAGGCAGGACGACGTACAGGGCGCCAGCCTCAGC
CATCACAGCCCGATGTTGCTGCACTGCATGAAGCAATGGCGCCTGAATCCCAGCCTCCGTCCGCCAACGCTCAAGGTGGT
GCCGGATGGCCCGACGGTGCAACCCAGCTTTATCGGCCTCAACCGCGAAGACGACCAGCACGTGCTGATCTTCCTCGAAG
ACATTTCGCAGATCGCCCAGCAGGCGCAGCAGATGAAGCTGGCCGGACTCGGCCGCCTGACCGCCGGCATCGCCCATGAG
ATCCGCAACCCGCTGGGCGCGATCAGCCACGCCGCCCAACTGCTGCAAGAGTCCGAGGAACTGGATGCCCCCGACCGGCG
CCTGACGCAGATCATCCAGGACCAGTCGAAGCGGATGAACCTGGTCATCGAGAACGTCCTGCAGCTCTCCCGTCGCCGCC
AGGCCGAACCGCAGCAGCTCGACCTGAAGGAGTGGCTGCGGCGGTTCGTCGACGAATACCCCGGCAGGCTGCGCAACGAC
AGCGAACTGCACCTGCAACTCGGTGACGGCGACATCCAGACCCGCATGGACCCACATCAGTTGAACCAGGTGCTGAGCAA
CCTGGTGCAGAACGGTCTTCGCTACAGCGCCCAGGCGCACGGGCGCGGCCAGGTCTGGCTGAGCCTCGCGCGCGACCCGG
AGAGCGACCTGCCGGTGCTGGAAGTCATCGACGACGGTCCCGGCGTAGCGACGGACAAACTGAACAACCTGTTCGAACCC
TTCTTTACTACAGAAAGCAAAGGTACCGGCCTGGGCCTCTATCTCTCCCGCGAACTCTGCGAGAGCAACCAGGCACGGAT
CGACTACCGCAATCGCGAGGAAGGCGGCGGCTGCTTCCGCATCACCTTCGCCCACCCGCGCAAACTCAGCTGA

Domains

Predicted by InterproScan.

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c)

(417-523)

His Kinase A (phospho-acceptor) domain (HisKA)

(310-373)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilS Pseudomonas aeruginosa PAK

97.925

100

0.979

Multiple sequence alignment