Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   GNK04_RS08745 Genome accession   NZ_CP046564
Coordinates   1652489..1655980 (+) Length   1163 a.a.
NCBI ID   WP_159782112.1    Uniprot ID   A0A7Z2QBD7
Organism   Bacillus sp. N1-1     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1647489..1660980
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GNK04_RS08710 (GNK04_08710) - 1647783..1649033 (+) 1251 WP_159782108.1 hypothetical protein -
  GNK04_RS08715 (GNK04_08715) - 1649265..1649720 (+) 456 WP_240904095.1 pyridoxamine 5'-phosphate oxidase family protein -
  GNK04_RS08720 (GNK04_08720) - 1649823..1650449 (+) 627 WP_159782110.1 TVP38/TMEM64 family protein -
  GNK04_RS08725 (GNK04_08725) cotJC 1650917..1651486 (-) 570 WP_159782111.1 spore coat protein CotJC -
  GNK04_RS08730 (GNK04_08730) - 1651508..1651774 (-) 267 WP_098443060.1 spore coat protein CotJB -
  GNK04_RS08735 (GNK04_08735) - 1651778..1651993 (-) 216 WP_098443061.1 spore coat associated protein CotJA -
  GNK04_RS08740 (GNK04_08740) - 1652105..1652299 (-) 195 WP_098443062.1 hypothetical protein -
  GNK04_RS08745 (GNK04_08745) addB 1652489..1655980 (+) 3492 WP_159782112.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  GNK04_RS08750 (GNK04_08750) addA 1655970..1659680 (+) 3711 WP_240904096.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  GNK04_RS08755 (GNK04_08755) - 1659743..1660903 (+) 1161 WP_159782114.1 exonuclease SbcCD subunit D -

Sequence

Protein


Download         Length: 1163 a.a.        Molecular weight: 134051.64 Da        Isoelectric Point: 5.1703

>NTDB_id=405539 GNK04_RS08745 WP_159782112.1 1652489..1655980(+) (addB) [Bacillus sp. N1-1]
MTVKFVIGRSGSGKSNHCLTEMQNELTDRPIGQDMIYLVPEQMTFQSEYELVHNRGLKGMIRAQVFSFTRLAWKVLQETG
GMARPHLSSVGTSMMLRQIIEENKQKLRIYQRSSEKTGYVEQLNDMVTEFKRYCLEPDDIKSFAEDFYAEDERNLLRDKL
YDLHLVYEAFQKKMIGHYLDSEDYLALLAEKVEESDYLTNATIWIDGFHSFTPQELLVIQSLMKKGTTLTFTITMDSEMI
PSAIHELDLFHEPGKTYQILQGMANEEGVEIEEPLVLTTQKRYRNSEIAHLEANYGERPPVVYRGELQHIQVHHAVNIRS
EVEAVAREALRLVRDEGYRYRDISIMVRNMSTYYELVETIFEDHGIPIFSDQKRTMLHHPLIELIRSSLDIIQYNWRYES
VFRCVKTELLFPDDTGESIETIRERMDELENYVIAKGIKGYRWKTGERFKANIYRQLEDKEVITSDELEKMEDRLNETKD
WIATPLEKFERHMTKAKTVEQMCEILYYLLENCHAAEKIDRLKEQAEKSGHLAKAREHDQVWDAVISLLDETVELIGDQK
LSVEQFTKLLEAGMENMKFALVPASLDQVVIGSIDRTRFTDVKCGFLLGVNEGVLPARIQEDGLLSEQDREKLEEQGIQL
APGLKRRLLDEEFLIYHSLATPSDSLWVSCPLADEEGKGLQPSIVVNRLQEMFPDLELGLKFAEPGEEATEELSFITNPS
KTIGRLTGQLRQWHRGYPIDPIWWDAYNWFISAPSEKDRVRLIDSLFYRNKADRLTESTSRKLYGHQIQTSVSRMERYKS
CAFSQFASHGLRLKERATFKLEAPDIGQLFHAALNMMAETIKQRHWDWAKLSSNQYYELSGQIVEQLAPKLQNEILLSSN
RYHYIMRKLTQVVGRASSVLGQQAKKSGFQPAGLELGFGPNQELPPIEFTLKNGVKIQLIGRVDRVDQALNGEELLLRII
DYKSSGTSLDLSEVYFGLALQMLTYLDVVISNANVWLGREATAAGMLYFHVHNPMLQTSAPGQAAIQQELFKKFKMKGLL
LADKDVVSLMDNDIETKKSDIIPAALKKDGSFYKNSSVLSSDDFNALRSYTRKTIQSIGSEISDGRIEIDPYKMKDRIPC
TFCSYRSFCQFDQAMEDNAYRPIATQDDETILRRMREEADDDN

Nucleotide


Download         Length: 3492 bp        

>NTDB_id=405539 GNK04_RS08745 WP_159782112.1 1652489..1655980(+) (addB) [Bacillus sp. N1-1]
ATGACAGTAAAATTTGTCATTGGTCGTTCAGGAAGTGGGAAATCGAATCATTGTTTAACGGAAATGCAAAATGAACTAAC
CGATCGGCCAATCGGACAGGATATGATTTACCTCGTTCCTGAGCAAATGACGTTTCAATCAGAGTATGAATTAGTACATA
ATAGAGGTTTAAAAGGAATGATTCGAGCGCAGGTTTTTAGTTTCACTAGACTTGCCTGGAAAGTGCTTCAAGAAACTGGG
GGAATGGCTCGTCCTCATTTAAGTAGTGTCGGAACAAGTATGATGCTTCGACAGATCATTGAGGAAAATAAGCAGAAACT
CCGAATTTATCAGCGTTCTTCTGAGAAAACAGGGTATGTGGAACAGCTAAATGATATGGTAACCGAATTTAAGCGGTATT
GTCTGGAACCAGATGATATCAAGAGCTTTGCCGAGGACTTTTATGCAGAAGATGAGCGGAACTTGCTTCGAGATAAGCTC
TATGACTTGCATCTTGTTTACGAAGCTTTTCAAAAAAAGATGATAGGTCACTACCTCGATTCAGAAGACTACTTAGCACT
GCTAGCGGAGAAAGTTGAGGAGTCAGACTATCTAACGAACGCTACAATTTGGATAGACGGCTTTCATAGTTTTACCCCTC
AGGAACTACTGGTCATTCAATCATTAATGAAAAAAGGAACAACCCTTACGTTTACGATTACGATGGATTCTGAAATGATC
CCTTCTGCTATTCATGAACTCGATCTGTTTCATGAACCAGGAAAAACATATCAAATACTTCAGGGAATGGCGAATGAAGA
AGGTGTAGAAATCGAGGAACCTCTCGTTTTAACAACACAAAAGAGGTATCGAAACAGTGAGATCGCTCACTTAGAAGCCA
ATTATGGTGAACGGCCGCCGGTTGTTTATCGTGGAGAGTTGCAGCACATACAAGTTCATCATGCGGTGAATATACGTTCT
GAAGTTGAGGCAGTAGCACGAGAAGCGCTAAGACTTGTTCGAGATGAAGGTTATCGTTACAGAGATATTTCAATCATGGT
CCGCAATATGTCAACTTATTATGAACTTGTGGAAACGATATTCGAGGATCACGGTATTCCAATTTTCTCAGATCAAAAGC
GGACAATGCTTCATCACCCGTTAATTGAGTTGATTCGCTCAAGTTTAGACATTATCCAGTACAACTGGCGGTATGAATCG
GTTTTTCGATGTGTCAAAACTGAGCTATTGTTTCCTGATGATACAGGGGAAAGCATTGAAACCATTCGCGAACGGATGGA
TGAGCTTGAAAATTACGTTATAGCAAAAGGCATCAAAGGCTATCGTTGGAAGACTGGTGAAAGATTTAAAGCAAATATTT
ATCGTCAGTTAGAAGATAAAGAAGTCATCACATCCGATGAACTCGAAAAAATGGAAGATCGCCTGAATGAAACGAAAGAC
TGGATTGCGACGCCGCTTGAGAAATTTGAGCGACACATGACGAAAGCAAAAACCGTTGAACAAATGTGCGAAATTCTTTA
TTATCTTCTTGAAAATTGCCACGCAGCGGAAAAAATCGATCGTTTAAAAGAGCAGGCAGAAAAGTCCGGTCACCTTGCCA
AAGCACGTGAACATGATCAGGTTTGGGACGCGGTGATTTCGCTACTTGACGAAACAGTCGAATTAATCGGTGATCAGAAA
CTCTCCGTGGAGCAATTTACAAAGTTGCTTGAAGCCGGAATGGAAAATATGAAATTTGCTCTCGTACCTGCTTCACTTGA
CCAGGTAGTGATTGGAAGCATTGATCGAACAAGATTTACTGATGTAAAATGTGGCTTTCTTTTAGGTGTAAATGAAGGTG
TCTTACCGGCTCGAATTCAGGAAGATGGACTGTTAAGTGAGCAGGATAGGGAAAAGTTAGAAGAACAGGGAATTCAGTTA
GCTCCTGGGTTGAAGCGGCGTTTGCTTGATGAAGAATTTTTAATTTACCATTCGTTAGCAACACCTTCGGATTCTTTATG
GGTTAGCTGTCCGCTTGCTGATGAAGAAGGAAAGGGTCTCCAGCCTTCTATCGTCGTGAATCGATTGCAGGAGATGTTTC
CTGATCTTGAGCTGGGACTGAAATTTGCAGAGCCAGGTGAAGAAGCCACGGAAGAACTCTCATTTATAACAAACCCATCC
AAGACAATTGGGCGACTCACTGGACAGCTTCGACAGTGGCACCGAGGGTATCCGATTGATCCAATTTGGTGGGATGCATA
CAACTGGTTTATTTCGGCTCCATCTGAGAAAGATCGCGTACGCTTAATTGATAGTCTTTTTTATCGAAATAAAGCCGATC
GTCTAACTGAATCAACAAGCAGAAAACTTTATGGTCATCAAATTCAGACGAGTGTTTCAAGGATGGAAAGATATAAATCA
TGTGCTTTCTCTCAGTTTGCTTCACATGGTTTACGCTTAAAAGAACGGGCGACATTCAAGCTTGAAGCACCTGACATCGG
ACAGCTGTTTCATGCGGCGCTCAATATGATGGCAGAAACGATTAAGCAGCGTCATTGGGATTGGGCGAAACTTTCATCTA
ATCAATACTATGAGCTTTCAGGACAAATCGTTGAACAGCTCGCACCAAAGTTACAGAATGAAATTCTATTAAGTTCAAAT
CGCTATCACTATATTATGAGAAAGCTAACGCAAGTTGTTGGGCGCGCCTCGTCCGTTCTTGGACAACAGGCAAAGAAAAG
TGGGTTTCAACCAGCAGGACTCGAATTAGGATTTGGTCCCAATCAAGAGCTTCCTCCAATCGAGTTCACGCTTAAAAATG
GTGTTAAAATTCAGCTCATCGGACGAGTCGATCGAGTTGATCAGGCACTGAACGGAGAAGAGCTTCTCCTTCGTATTATC
GACTATAAGTCTAGCGGTACATCTCTTGATCTATCGGAAGTGTATTTTGGACTCGCCCTACAAATGCTGACGTATCTGGA
TGTTGTTATATCTAATGCGAACGTCTGGCTTGGAAGAGAGGCAACTGCTGCAGGTATGCTTTATTTTCATGTCCACAACC
CTATGCTCCAAACGTCAGCGCCTGGACAAGCGGCAATTCAACAGGAGCTTTTCAAAAAGTTTAAGATGAAGGGACTTCTC
CTTGCGGATAAAGACGTTGTGAGCTTAATGGATAACGATATTGAAACGAAGAAATCGGATATTATCCCGGCCGCACTGAA
AAAAGACGGTAGCTTTTACAAAAACTCTTCCGTCTTATCTTCTGATGATTTTAATGCGCTTCGTTCTTATACAAGGAAAA
CAATTCAATCGATTGGGTCGGAAATTTCAGATGGCCGTATTGAGATTGATCCATATAAAATGAAAGATCGAATACCATGT
ACGTTCTGTTCTTACCGATCCTTTTGCCAGTTTGATCAGGCGATGGAAGATAATGCTTATCGTCCTATTGCTACTCAGGA
TGATGAAACGATATTAAGACGCATGAGAGAGGAGGCAGATGATGATAACTAA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(790-1130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7Z2QBD7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

49.102

100

0.494

Multiple sequence alignment