Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   GNK04_RS08750 Genome accession   NZ_CP046564
Coordinates   1655970..1659680 (+) Length   1236 a.a.
NCBI ID   WP_240904096.1    Uniprot ID   -
Organism   Bacillus sp. N1-1     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1650970..1664680
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GNK04_RS08730 (GNK04_08730) - 1651508..1651774 (-) 267 WP_098443060.1 spore coat protein CotJB -
  GNK04_RS08735 (GNK04_08735) - 1651778..1651993 (-) 216 WP_098443061.1 spore coat associated protein CotJA -
  GNK04_RS08740 (GNK04_08740) - 1652105..1652299 (-) 195 WP_098443062.1 hypothetical protein -
  GNK04_RS08745 (GNK04_08745) addB 1652489..1655980 (+) 3492 WP_159782112.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  GNK04_RS08750 (GNK04_08750) addA 1655970..1659680 (+) 3711 WP_240904096.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  GNK04_RS08755 (GNK04_08755) - 1659743..1660903 (+) 1161 WP_159782114.1 exonuclease SbcCD subunit D -
  GNK04_RS08760 (GNK04_08760) - 1660919..1664272 (+) 3354 WP_159782115.1 SMC family ATPase -

Sequence

Protein


Download         Length: 1236 a.a.        Molecular weight: 143303.46 Da        Isoelectric Point: 4.9041

>NTDB_id=405540 GNK04_RS08750 WP_240904096.1 1655970..1659680(+) (addA) [Bacillus sp. N1-1]
MITKPEGSRWTDEQWEAIASRNQDVLVAAAAGSGKTAVLVERIIRRLADENDPAEVDRILVVTFTNAAAAEMRHRIGEAL
EEKLKEDPSSLYLRRQLSMLNRASISTLHSFCMEVLRRYYYKIDLDPAFRVADQTEAALLREEAMEELFEEHYSQKENEA
FYDLVDRYSSDRSDVDLQLLVERLFHFSSSHPWPKQWLVDSVDLYKNAGDKRFDELIWVEELKKDVVLQLEGMREVQEKA
ISISSSPGGPEPYLSTLEVEREMVNGLLYASKESWEALKNAFEAVSFGRLKPCKGDQFDPELIDAVKGMRDRVKKIVQSM
KEDLFQREAEEYLEDIQKLAPVLETLASLVNEFRDKYTDLKKEKGLVDYSDLEHYCLSILLDEASTPDNPIASSAAKEYQ
TQFAEVLIDEYQDTNSVQETILNAISRTSEHGGNRFMVGDVKQSIYRFRLAEPGLFLAKYKSFGRSSEEPTKRIDLARNF
RSREEVLNSTNFIFKQIMDERVGEIKYDRAAELITGAEYPKSQSVVPEVHVIDQSSDEEEEEVEKVQLEARLIASNIKSM
MGKSENERTKIYDRKEDRMRPIQYRDMVILMRATSSWAPVMMEELKQAGIPSYSDLATGYFEATEVAIMMSLLNVIDNPY
QDIPLASVLRSPIIGLSEEELALIRINEKQSSYYLAMQAYCETESDELSEKITRFIDDLNRWREQARQSSLSELIWQLYR
ETNYYEFVGGMPAGLQRQANLRALYDRARQYEATSFRGLFRFLRFIDRMKERGSDLGTARALGEQEDVVRVMTIHKSKGL
EFPVVFVAGIAKQFNMRDMANQMLLHKEFGLATKYIDPINRISYPTLPYFTLQKRMKLELLAEEMRVLYVALTRAKEKLI
LVGTVNDWEKELLSWAEAPGEEWLLSDYERSTSKTYLNWIGPAIIRHQDLSSITEGIASFPKHDEVFHHPSRWSLTLHQA
SDFSQVEEEDVKKHEEYEELLRRKAYVPEESAFKDVVHNRLNWTYPHQAAAETRSKQSVTEVKRQREIFQDERSDDRLVK
GFSQSLKERPRFLQSKKLTPAERGTAMHIVMQHIDLQQRHEENDIKELIAKLETKELLTNDQAQAIDVKQIKHLIDSDLG
DKMRNAVEIYKEVPFSLGVDSKWIYPDFKGDQETVVLQGVIDCILRDQHGELRLVDYKTDVIENRFSSEQQAMETMKRRY
TTQLQLYEKAINEIWGLPCKEKILFFFDGGKSLLID

Nucleotide


Download         Length: 3711 bp        

>NTDB_id=405540 GNK04_RS08750 WP_240904096.1 1655970..1659680(+) (addA) [Bacillus sp. N1-1]
ATGATAACTAAACCTGAAGGATCAAGATGGACGGATGAGCAGTGGGAAGCCATTGCTTCAAGAAATCAAGATGTTTTAGT
AGCTGCAGCGGCAGGATCCGGAAAAACCGCTGTTTTAGTTGAACGAATTATAAGGCGATTAGCCGATGAAAATGATCCTG
CTGAAGTTGATCGAATTCTAGTCGTAACATTTACAAATGCAGCAGCTGCTGAAATGCGCCACCGAATTGGAGAAGCGCTC
GAAGAAAAGCTGAAGGAAGATCCTTCTTCGCTTTATTTAAGAAGACAGCTTTCCATGTTAAATCGTGCTTCTATTTCTAC
ACTACATTCGTTTTGTATGGAAGTTCTTCGCCGCTATTATTACAAGATAGATCTTGACCCTGCTTTTCGCGTAGCTGATC
AAACGGAAGCCGCTTTACTTCGTGAAGAAGCGATGGAAGAGCTTTTTGAAGAGCACTACAGCCAGAAAGAAAATGAAGCG
TTTTATGACCTCGTCGATCGCTATAGTAGCGATCGTAGCGATGTCGATCTTCAGCTTCTAGTTGAACGGCTTTTTCATTT
CTCTTCAAGTCATCCTTGGCCGAAACAGTGGTTGGTAGATAGTGTAGATTTATATAAAAATGCAGGTGATAAGCGCTTCG
ATGAGCTGATCTGGGTAGAGGAATTAAAGAAAGATGTTGTGCTTCAGTTAGAAGGAATGCGTGAAGTGCAGGAAAAGGCC
ATTTCCATTTCATCTTCCCCAGGTGGTCCAGAACCGTACCTTTCAACCTTGGAAGTCGAGCGCGAAATGGTGAATGGACT
TCTTTACGCTTCTAAGGAATCATGGGAAGCATTAAAAAACGCGTTTGAAGCTGTTTCATTTGGACGCTTAAAGCCTTGTA
AAGGTGACCAGTTTGATCCTGAGCTTATTGACGCCGTCAAAGGAATGCGTGATCGGGTTAAGAAAATTGTTCAAAGTATG
AAAGAAGATCTGTTTCAACGGGAAGCAGAGGAGTATCTCGAGGATATCCAGAAACTTGCGCCTGTTCTTGAAACGTTAGC
TTCTCTCGTAAATGAATTTAGGGATAAGTATACTGATCTTAAGAAAGAAAAGGGACTTGTTGACTATAGTGACCTTGAGC
ATTACTGTCTTTCGATTTTATTAGATGAGGCATCAACACCTGATAACCCTATCGCTTCAAGTGCGGCGAAGGAATATCAG
ACGCAGTTCGCCGAAGTGCTCATTGATGAGTACCAGGATACGAACTCTGTTCAAGAAACGATTCTTAATGCCATTAGCCG
AACTTCCGAACACGGTGGGAATCGTTTTATGGTAGGTGATGTTAAACAAAGTATATACAGGTTTAGACTGGCTGAGCCCG
GGTTATTTCTAGCAAAATATAAGTCGTTTGGCCGCTCTTCAGAGGAGCCTACTAAACGGATCGATCTTGCACGAAACTTT
CGAAGTCGTGAGGAAGTTTTAAATAGCACCAACTTTATTTTTAAACAAATTATGGATGAACGCGTTGGGGAAATTAAATA
TGATCGTGCAGCTGAACTTATTACTGGTGCAGAGTATCCCAAGTCTCAATCGGTGGTACCAGAAGTCCATGTGATTGATC
AAAGCTCTGATGAAGAGGAAGAAGAGGTCGAAAAAGTTCAGCTTGAAGCACGTCTGATCGCATCCAACATTAAATCCATG
ATGGGAAAGAGCGAAAACGAGCGAACGAAAATATACGACCGAAAAGAAGATCGCATGAGACCCATTCAGTACAGAGATAT
GGTGATCTTAATGCGCGCCACTTCTAGCTGGGCACCGGTTATGATGGAGGAATTAAAGCAGGCGGGTATTCCATCTTATT
CAGACCTTGCGACAGGGTATTTTGAAGCGACAGAAGTAGCGATTATGATGAGTCTGCTAAACGTGATCGATAATCCCTAT
CAGGATATCCCGCTCGCATCTGTCTTGCGATCACCGATTATTGGGTTATCGGAAGAAGAACTTGCATTGATTCGGATAAA
TGAGAAGCAATCAAGTTATTATCTCGCCATGCAGGCGTATTGTGAGACAGAGTCGGATGAGCTTTCAGAGAAAATAACAC
GGTTTATCGATGATTTAAATCGTTGGCGAGAACAGGCAAGGCAGTCGTCGTTAAGCGAATTAATTTGGCAATTGTATCGT
GAAACGAATTACTATGAATTTGTCGGCGGAATGCCTGCTGGTTTGCAGCGCCAGGCGAATCTGCGTGCTTTGTATGATCG
TGCAAGACAATACGAAGCAACTTCATTTCGTGGATTGTTCCGCTTTCTTCGCTTTATTGACAGAATGAAGGAGCGGGGAA
GCGATCTCGGAACAGCCCGTGCGCTTGGAGAGCAGGAAGATGTTGTGCGCGTGATGACGATTCATAAAAGCAAAGGACTT
GAATTCCCTGTTGTCTTTGTCGCAGGCATAGCAAAGCAATTCAACATGAGAGACATGGCAAACCAAATGCTTCTCCATAA
AGAATTCGGTTTAGCAACGAAATACATTGATCCAATCAATCGTATTAGCTATCCGACGTTGCCTTACTTTACATTACAAA
AGCGAATGAAGCTTGAGCTATTAGCTGAAGAAATGCGGGTGCTTTACGTTGCTTTGACGAGAGCAAAAGAAAAATTAATT
TTAGTCGGCACGGTAAATGACTGGGAGAAAGAATTACTCTCCTGGGCAGAGGCACCAGGAGAGGAGTGGCTCCTTTCCGA
TTATGAGCGCTCAACAAGTAAAACGTATTTGAACTGGATTGGGCCAGCTATTATCAGGCATCAAGACCTTTCTTCAATAA
CAGAGGGAATAGCTAGTTTCCCGAAACATGATGAGGTTTTTCATCACCCATCACGATGGTCGTTAACGCTCCATCAAGCT
TCGGATTTTTCTCAAGTTGAAGAAGAAGACGTTAAGAAACACGAGGAATACGAGGAACTCCTTCGAAGGAAAGCCTACGT
TCCAGAAGAAAGTGCCTTTAAAGACGTTGTTCATAATCGTCTTAATTGGACATATCCACACCAGGCAGCTGCTGAAACAA
GATCGAAGCAGTCTGTAACGGAAGTGAAGCGTCAGCGGGAAATTTTCCAGGACGAACGTAGTGACGATCGCCTAGTAAAA
GGCTTTAGCCAATCTCTAAAAGAACGCCCCCGCTTTCTTCAAAGTAAAAAGCTTACGCCTGCTGAGCGCGGAACCGCGAT
GCATATCGTCATGCAGCACATTGATTTACAACAGCGGCATGAAGAAAATGATATTAAAGAATTAATCGCAAAGCTTGAAA
CGAAAGAATTGTTAACAAACGATCAGGCTCAAGCCATTGATGTGAAGCAAATCAAGCACTTGATTGATTCTGATCTAGGT
GACAAGATGAGAAACGCGGTCGAAATATATAAGGAGGTTCCCTTTAGTCTTGGGGTGGATTCAAAGTGGATTTATCCAGA
TTTCAAAGGAGACCAAGAAACGGTCGTGTTACAGGGTGTTATTGACTGTATCTTGCGCGATCAACACGGAGAATTACGAT
TAGTCGATTATAAAACAGACGTGATTGAAAATCGTTTTTCTTCAGAGCAGCAGGCAATGGAGACAATGAAACGTCGTTAT
ACAACACAGCTTCAACTATATGAAAAAGCGATTAATGAGATTTGGGGTTTGCCATGTAAAGAAAAAATTCTTTTCTTTTT
TGATGGTGGCAAATCACTCTTAATCGATTAA

Domains

Predicted by InterproScan.

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(10-463)

UvrD-like helicase C-terminal domain (UvrD_C)

(474-882)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1051-1214)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

50.482

100

0.508

Multiple sequence alignment