Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   HUU85_RS07350 Genome accession   NZ_CP058302
Coordinates   1531584..1532243 (+) Length   219 a.a.
NCBI ID   WP_001221493.1    Uniprot ID   Q3YXL4
Organism   Escherichia coli strain AH65     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 1526584..1537243
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HUU85_RS07325 (HUU85_07330) ygiS 1527867..1529474 (-) 1608 WP_001295629.1 ABC transporter substrate-binding protein -
  HUU85_RS07330 (HUU85_07335) mqsA 1529607..1530002 (-) 396 WP_000650107.1 type II toxin-antitoxin system antitoxin MqsA -
  HUU85_RS07335 (HUU85_07340) mqsR 1530004..1530300 (-) 297 WP_000415584.1 type II toxin-antitoxin system toxin MqsR -
  HUU85_RS07340 (HUU85_07345) ygiV 1530505..1530987 (-) 483 WP_000183505.1 GyrI-like domain-containing protein -
  HUU85_RS07345 (HUU85_07350) ygiW 1531040..1531432 (-) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  HUU85_RS07350 (HUU85_07355) ciaR 1531584..1532243 (+) 660 WP_001221493.1 quorum sensing response regulator transcription factor QseB Regulator
  HUU85_RS07355 (HUU85_07360) qseC 1532240..1533589 (+) 1350 WP_000673402.1 quorum sensing histidine kinase QseC -
  HUU85_RS07360 (HUU85_07365) ygiZ 1533635..1533968 (-) 334 Protein_1451 DUF2645 family protein -
  HUU85_RS07365 (HUU85_07370) mdaB 1534287..1534868 (+) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  HUU85_RS07370 (HUU85_07375) ygiN 1534899..1535213 (+) 315 WP_000958598.1 putative quinol monooxygenase -
  HUU85_RS07375 (HUU85_07380) parE 1535261..1537153 (-) 1893 WP_000195296.1 DNA topoisomerase IV subunit B -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24687.63 Da        Isoelectric Point: 6.9850

>NTDB_id=404604 HUU85_RS07350 WP_001221493.1 1531584..1532243(+) (ciaR) [Escherichia coli strain AH65]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=404604 HUU85_RS07350 WP_001221493.1 1531584..1532243(+) (ciaR) [Escherichia coli strain AH65]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAAGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGTT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q3YXL4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365