Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   GFX43_RS08940 Genome accession   NZ_CP046448
Coordinates   1730343..1730960 (+) Length   205 a.a.
NCBI ID   WP_015384132.1    Uniprot ID   -
Organism   Bacillus subtilis strain ZD01     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1725343..1735960
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GFX43_RS08905 (GFX43_008905) aroE 1726001..1726843 (+) 843 WP_015384136.1 shikimate dehydrogenase -
  GFX43_RS08910 (GFX43_008910) yhbY 1726837..1727127 (+) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  GFX43_RS08915 (GFX43_008915) nadD 1727139..1727708 (+) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  GFX43_RS08920 (GFX43_008920) yqeK 1727698..1728258 (+) 561 WP_015483448.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  GFX43_RS08925 (GFX43_008925) rsfS 1728276..1728632 (+) 357 WP_014480315.1 ribosome silencing factor -
  GFX43_RS08930 (GFX43_008930) yqeM 1728629..1729372 (+) 744 WP_015384134.1 class I SAM-dependent methyltransferase -
  GFX43_RS08935 (GFX43_008935) comER 1729438..1730259 (-) 822 WP_004398597.1 late competence protein ComER -
  GFX43_RS08940 (GFX43_008940) comEA 1730343..1730960 (+) 618 WP_015384132.1 competence protein ComEA Machinery gene
  GFX43_RS08945 (GFX43_008945) comEB 1731027..1731596 (+) 570 WP_003229978.1 ComE operon protein 2 -
  GFX43_RS08950 (GFX43_008950) comEC 1731600..1733930 (+) 2331 WP_041517945.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  GFX43_RS08955 (GFX43_008955) yqzM 1733970..1734104 (-) 135 WP_003229983.1 YqzM family protein -
  GFX43_RS08960 (GFX43_008960) - 1734145..1734294 (+) 150 WP_003229985.1 hypothetical protein -
  GFX43_RS08965 (GFX43_008965) holA 1734334..1735377 (+) 1044 WP_015384130.1 DNA polymerase III subunit delta -
  GFX43_RS08970 (GFX43_008970) rpsT 1735392..1735658 (-) 267 WP_003229989.1 30S ribosomal protein S20 -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21744.44 Da        Isoelectric Point: 4.7155

>NTDB_id=404390 GFX43_RS08940 WP_015384132.1 1730343..1730960(+) (comEA) [Bacillus subtilis strain ZD01]
MNWLNQHKKAIVLAASAAVFTAIMIFLATGKNKDLVKQAIPTETENTVVKQEANNDESSETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=404390 GFX43_RS08940 WP_015384132.1 1730343..1730960(+) (comEA) [Bacillus subtilis strain ZD01]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTGTTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGACCTGGTGAAGCAAGCTATACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAGCGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTTTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCCTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(63-118)

Helix-hairpin-helix motif (HHH_3)

(142-203)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

97.561

100

0.976
  comEA Staphylococcus aureus N315

33.784

100

0.366

Multiple sequence alignment