Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilS   Type   Regulator
Locus tag   MDS_RS05275 Genome accession   NC_015410
Coordinates   1159056..1160648 (-) Length   530 a.a.
NCBI ID   WP_013714193.1    Uniprot ID   A0A379IYZ0
Organism   Ectopseudomonas mendocina NK-01     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 1154056..1165648
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MDS_RS05245 (MDS_1048) - 1154167..1154889 (-) 723 WP_013714187.1 PilW family protein -
  MDS_RS05250 (MDS_1049) pilV 1154889..1155404 (-) 516 WP_013714188.1 type IV pilus modification protein PilV -
  MDS_RS05255 (MDS_1050) - 1155401..1155889 (-) 489 WP_013714189.1 GspH/FimT family pseudopilin -
  MDS_RS05260 (MDS_1051) - 1156005..1156508 (-) 504 WP_041769348.1 GspH/FimT family pseudopilin -
  MDS_RS05265 (MDS_1052) thiO 1156599..1157696 (+) 1098 WP_041769349.1 glycine oxidase ThiO -
  MDS_RS05270 (MDS_1053) pilR 1157713..1159053 (-) 1341 WP_013714192.1 sigma-54 dependent transcriptional regulator Regulator
  MDS_RS05275 (MDS_1054) pilS 1159056..1160648 (-) 1593 WP_013714193.1 PAS domain-containing sensor histidine kinase Regulator
  MDS_RS05280 (MDS_1055) - 1160638..1160874 (-) 237 WP_013714194.1 PP0621 family protein -
  MDS_RS05285 (MDS_1056) - 1161070..1162062 (-) 993 WP_013714195.1 outer membrane protein assembly factor BamD -
  MDS_RS05290 (MDS_1057) rluD 1162201..1163175 (+) 975 WP_041769670.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  MDS_RS05295 (MDS_1058) pgeF 1163172..1163903 (+) 732 WP_013714197.1 peptidoglycan editing factor PgeF -
  MDS_RS05300 (MDS_1059) - 1164036..1164626 (+) 591 WP_237705687.1 TetR/AcrR family transcriptional regulator -

Sequence

Protein


Download         Length: 530 a.a.        Molecular weight: 58975.11 Da        Isoelectric Point: 8.1909

>NTDB_id=40397 MDS_RS05275 WP_013714193.1 1159056..1160648(-) (pilS) [Ectopseudomonas mendocina NK-01]
MTADLSLQGGTQGRRILRLYHFYRLSIGLALILLISSNLDEQFLDVVHIELFRTGSWAYLILNILIAVLVQKPKHLMQLF
SLALVDVIFLSALFYAAGGTPSGIGNLLVVAVAIANILLRGRIGLLIAAIAAIALIYLTFYLSLSRPAAAAQYVQAGALG
ALCFAGALFIQGITRRLQVSESLAEQRAADVANLEELNEQILQRMRTGILVLDTQHRVLLANQGATQLLGRGELTGKIID
PHCPELVKRLQQWLHNPTLRPDSLQAQVDGPVLQPSFISLQRGEQQHILVFLDDISQIAQQAQQLKLASLGRLTAGIAHE
IRNPLGAISHAAQLLQESEDLQGPDQRLAQIIQDHSKRMNLVIENVLQLSRRRQAEPQLLDLKYWLHRFASEFRSSAPAD
RQIHLETQGGSLQTRMDPHQLTQVLTNLVQNGLRYSAQKHRLGQVWLRLFRDESSDLPVLEILDDGPGVPPEQEQHIFEP
FYTTENKGTGLGLYISRELCQGNQARLDYKPREGGGSCFRITFAHPRKLS

Nucleotide


Download         Length: 1593 bp        

>NTDB_id=40397 MDS_RS05275 WP_013714193.1 1159056..1160648(-) (pilS) [Ectopseudomonas mendocina NK-01]
GTGACCGCTGACCTCAGCTTGCAAGGCGGTACGCAGGGGCGACGCATCCTCAGGCTTTATCACTTCTATCGCCTGAGCAT
CGGCCTGGCCCTGATCCTGCTGATATCCAGCAATCTCGATGAACAGTTTCTGGATGTGGTGCATATCGAGCTGTTTCGGA
CAGGCAGCTGGGCCTATCTGATCCTCAACATCCTCATCGCCGTGCTGGTACAAAAGCCCAAGCACCTGATGCAGCTGTTC
AGCCTGGCGCTGGTGGACGTGATTTTCCTGTCCGCGCTGTTCTATGCCGCTGGCGGCACGCCCAGCGGCATCGGCAACCT
GCTGGTGGTAGCGGTGGCCATCGCCAATATTCTGCTGCGCGGCCGCATCGGCCTGCTGATCGCCGCCATCGCTGCCATCG
CTCTTATTTACCTGACCTTCTATCTCAGCCTCAGCCGCCCCGCCGCTGCCGCCCAGTACGTGCAGGCCGGCGCACTTGGC
GCGCTGTGTTTCGCCGGCGCACTGTTTATCCAGGGCATCACTCGCCGCCTGCAGGTCAGCGAAAGCCTGGCCGAGCAACG
CGCGGCCGATGTCGCCAACCTAGAGGAGCTCAACGAGCAGATATTGCAGCGCATGCGCACCGGTATCCTGGTACTCGATA
CCCAGCATCGCGTGCTCCTGGCCAACCAGGGCGCTACGCAACTGCTGGGCCGCGGGGAGCTGACCGGCAAGATCATCGAC
CCGCACTGCCCGGAACTGGTCAAACGGCTGCAACAGTGGCTGCACAACCCGACGCTGCGCCCGGACAGCCTTCAGGCGCA
AGTCGATGGCCCGGTCCTGCAGCCCAGTTTCATTTCCCTGCAACGCGGCGAGCAGCAGCACATCCTGGTGTTTCTCGACG
ACATTTCGCAGATTGCCCAGCAGGCGCAACAGCTCAAACTGGCCTCGCTCGGCCGCTTGACCGCCGGTATCGCCCACGAG
ATTCGCAACCCGCTTGGTGCGATCAGCCACGCTGCACAGCTGCTGCAGGAATCGGAAGACCTGCAAGGTCCGGATCAACG
CCTAGCGCAGATCATTCAGGATCACTCCAAGCGCATGAATCTGGTGATCGAGAACGTCCTGCAGCTGTCGCGCCGGCGCC
AGGCCGAGCCGCAGCTACTGGACCTGAAATACTGGCTGCACCGCTTCGCCAGCGAGTTCCGTAGCTCGGCACCGGCTGAC
CGGCAGATCCATCTGGAAACCCAGGGCGGCAGTCTGCAAACACGCATGGACCCGCACCAGTTGACCCAGGTGCTGACCAA
CCTGGTGCAGAACGGCTTACGCTACAGCGCGCAGAAACATCGACTCGGCCAGGTCTGGCTACGCCTGTTCCGCGACGAGA
GCAGCGACCTGCCAGTGCTGGAAATACTCGACGACGGCCCCGGTGTGCCGCCCGAGCAGGAGCAGCACATCTTCGAGCCC
TTCTACACCACCGAGAACAAAGGCACCGGCCTCGGTCTGTATATTTCTCGCGAGCTGTGCCAGGGCAACCAGGCCCGCCT
CGACTATAAACCTCGTGAAGGTGGCGGCAGTTGCTTCCGCATCACCTTCGCCCATCCGCGCAAACTGAGCTGA

Domains

Predicted by InterproScan.

PAS domain (PAS_8)

(199-254)

His Kinase A (phospho-acceptor) domain (HisKA)

(309-373)

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c)

(417-523)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A379IYZ0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilS Pseudomonas aeruginosa PAK

67.63

97.925

0.662

Multiple sequence alignment