Detailed information    

insolico Bioinformatically predicted

Overview

Name   recG   Type   Machinery gene
Locus tag   HW370_RS00640 Genome accession   NZ_CP058222
Coordinates   127168..129249 (-) Length   693 a.a.
NCBI ID   WP_000678402.1    Uniprot ID   -
Organism   Escherichia coli strain CFT073     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 122168..134249
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HW370_RS00625 (HW370_00615) yicH 122428..124137 (-) 1710 WP_001304987.1 AsmA family protein -
  HW370_RS00630 (HW370_00620) xanP 124258..125649 (-) 1392 WP_001295238.1 xanthine/proton symporter XanP -
  HW370_RS00635 (HW370_00625) gltS 125929..127134 (+) 1206 WP_000468827.1 sodium/glutamate symporter -
  HW370_RS00640 (HW370_00630) recG 127168..129249 (-) 2082 WP_000678402.1 ATP-dependent DNA helicase RecG Machinery gene
  HW370_RS00645 (HW370_00635) trmH 129255..129944 (-) 690 WP_001070179.1 tRNA (guanosine(18)-2'-O)-methyltransferase TrmH -
  HW370_RS00650 (HW370_00640) spoT 129951..132059 (-) 2109 WP_000280473.1 bifunctional GTP diphosphokinase/guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase -
  HW370_RS00655 (HW370_00645) rpoZ 132078..132353 (-) 276 WP_000135058.1 DNA-directed RNA polymerase subunit omega -
  HW370_RS00660 (HW370_00650) gmk 132408..133031 (-) 624 WP_001295237.1 guanylate kinase -

Sequence

Protein


Download         Length: 693 a.a.        Molecular weight: 76462.41 Da        Isoelectric Point: 7.3383

>NTDB_id=403825 HW370_RS00640 WP_000678402.1 127168..129249(-) (recG) [Escherichia coli strain CFT073]
MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRYEDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRM
MTCQISDGSGILTMRFFNFNAAMKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQHPAQRRLILEELLAHNLSMLALR
AGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARVVAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQV
ALMAPTELLAEQHANNFRNWFAPLGIEVGWLAGKQKGKARLAQQEAIASGQVQMIVGTHAIFQEQVQFNGLALVIIDEQH
RFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDELPPGRTPVTTVAIPDTRRTDIIDRVRHACI
TEGRQAYWVCTLIEESELLEAQAAEATWEELKLALPELNVGLVHGRMKPADKQAVMASFKQGELHLLVATTVIEVGVDVP
NASLMIIENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQKDLEIRGPGELLGTRQ
TGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIERWMPETERYSNA

Nucleotide


Download         Length: 2082 bp        

>NTDB_id=403825 HW370_RS00640 WP_000678402.1 127168..129249(-) (recG) [Escherichia coli strain CFT073]
ATGAAAGGTCGCCTGTTAGATGCTGTCCCGCTCAGTTCCCTAACGGGCGTTGGCGCAGCGCTTAGTAACAAGCTGGCGAA
AATCAACCTGCATACCGTACAGGATTTGCTCTTACACCTTCCTCTGCGCTACGAAGATCGCACACATCTCTACCCCATCG
GTGAACTACTGCCGGGTGTTTATGCCACGGTGGAAGGCGAAGTGCTGAACTGCAATATCTCCTTCGGCGGTCGGCGGATG
ATGACCTGCCAGATCAGCGACGGTTCCGGCATTCTCACTATGCGCTTTTTCAACTTCAATGCGGCAATGAAAAATAGCCT
GGCGACAGGCCGCCGCGTGCTGGCCTATGGCGAAGCAAAGCGCGGCAAATATGGTGCGGAGATGATCCACCCGGAATACC
GCGTACAGGGCGATCTCAGCACGCCAGAGTTACAGGAAACGCTCACGCCGGTTTACCCAACTACGGAAGGCGTAAAGCAG
GCTACGCTGCGTAAATTAACCGACCAGGCGCTGGATCTGCTCGACACATGCGCCATTGAAGAACTCCTGCCGCCGGAACT
GTCACAAGGAATGATGACGCTACCGGAAGCGTTGCGCACTTTGCACCGCCCGCCGCCGACGCTACAGCTTAGCGATCTCG
AAACCGGGCAGCATCCGGCACAACGCCGGCTGATTCTGGAAGAGTTGCTGGCGCACAACCTCAGCATGTTGGCGTTACGT
GCCGGTGCACAACGTTTTCATGCTCAGCCGCTGAGCGCCAATGACGCGCTGAAAAATAAACTCCTCGCCGCCTTACCGTT
CAAGCCAACGGGCGCACAGGCACGCGTAGTGGCGGAGATCGAGCGCGATATGGCGCTGGATGTGCCGATGATGCGTCTGG
TGCAGGGCGATGTAGGTTCCGGTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATTGCGCACGGCAAACAGGTG
GCATTGATGGCACCAACCGAATTACTCGCCGAGCAGCACGCCAATAACTTCCGTAACTGGTTTGCTCCGCTCGGTATCGA
AGTGGGCTGGCTCGCCGGTAAACAGAAAGGTAAAGCACGACTGGCTCAGCAAGAAGCCATCGCCAGCGGTCAGGTGCAGA
TGATTGTTGGTACGCACGCCATTTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCAT
CGTTTTGGCGTGCATCAGCGTCTGGCGTTATGGGAGAAAGGCCAGCAGCAAGGCTTCCATCCGCATCAGTTGATCATGAC
CGCCACGCCCATCCCCCGCACGCTGGCGATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAG
GCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATTATTGACCGCGTGCGCCACGCCTGCATA
ACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAGCGCAGGCGGCGGAAGCAAC
CTGGGAAGAGTTGAAACTGGCGTTGCCGGAACTGAACGTCGGCCTGGTACACGGGCGGATGAAACCTGCCGATAAACAGG
CGGTGATGGCGTCGTTTAAACAAGGTGAACTACACCTGCTGGTTGCCACTACCGTTATTGAAGTCGGTGTTGATGTGCCT
AACGCCAGTCTGATGATTATCGAAAACCCGGAGCGTCTGGGTCTGGCGCAGTTACACCAGCTTCGCGGGCGTGTAGGTCG
TGGTGCGGTGGCTTCTCACTGCGTGCTGCTCTACAAAACGCCGCTCTCCAAAACCGCGCAAATTCGGCTGCAAGTGCTGC
GCGACAGCAACGACGGTTTTGTGATTGCACAGAAAGACTTAGAGATTCGCGGACCGGGTGAACTGTTAGGTACGCGTCAG
ACGGGTAATGCTGAATTTAAAGTGGCGGATTTACTGCGCGATCAGGCGATGATCCCGGAAGTTCAGCGCCTGGCGCGACA
TATTCACGAACGTTACCCACAACAGGCAAAAGCCCTGATAGAACGCTGGATGCCAGAAACAGAACGCTACTCGAATGCGT
AA

Domains

Predicted by InterProScan.

RecG wedge domain (RecG_wedge)

(15-171)

DEAD/DEAH box helicase (DEAD)

(273-433)

Helicase conserved C-terminal domain (Helicase_C)

(481-588)

ATP-dependent DNA helicase RecG, domain 3, C-terminal (RecG_dom3_C)

(617-676)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recG Neisseria meningitidis strain C311

52.232

96.97

0.506
  recG/mmsA Streptococcus pneumoniae R6

38.929

99.711

0.388
  recG/mmsA Streptococcus pneumoniae R36A

38.929

99.711

0.388
  recG Bacillus subtilis subsp. subtilis str. 168

39.058

94.949

0.371