Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   HUS69_RS03630 Genome accession   NZ_CP056073
Coordinates   756439..757098 (-) Length   219 a.a.
NCBI ID   WP_001221493.1    Uniprot ID   Q3YXL4
Organism   Escherichia coli strain EC50     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 751439..762098
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HUS69_RS03605 (HUS69_03615) parE 751530..753422 (+) 1893 WP_000195296.1 DNA topoisomerase IV subunit B -
  HUS69_RS03610 (HUS69_03620) ygiN 753470..753784 (-) 315 WP_000958598.1 putative quinol monooxygenase -
  HUS69_RS03615 (HUS69_03625) mdaB 753815..754396 (-) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  HUS69_RS03620 (HUS69_03630) ygiZ 754715..755047 (+) 333 WP_000917684.1 DUF2645 family protein -
  HUS69_RS03625 (HUS69_03635) qseC 755093..756442 (-) 1350 WP_000673402.1 quorum sensing histidine kinase QseC -
  HUS69_RS03630 (HUS69_03640) ciaR 756439..757098 (-) 660 WP_001221493.1 quorum sensing response regulator transcription factor QseB Regulator
  HUS69_RS03635 (HUS69_03645) ygiW 757250..757642 (+) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  HUS69_RS03640 (HUS69_03650) ygiV 757695..758177 (+) 483 WP_186240473.1 GyrI-like domain-containing protein -
  HUS69_RS03645 (HUS69_03655) mqsR 758382..758678 (+) 297 WP_000415584.1 type II toxin-antitoxin system toxin MqsR -
  HUS69_RS03650 (HUS69_03660) mqsA 758680..759075 (+) 396 WP_000650107.1 type II toxin-antitoxin system antitoxin MqsA -
  HUS69_RS03655 (HUS69_03665) ygiS 759208..760815 (+) 1608 WP_186240474.1 ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24687.63 Da        Isoelectric Point: 6.9850

>NTDB_id=403230 HUS69_RS03630 WP_001221493.1 756439..757098(-) (ciaR) [Escherichia coli strain EC50]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=403230 HUS69_RS03630 WP_001221493.1 756439..757098(-) (ciaR) [Escherichia coli strain EC50]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q3YXL4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365

Multiple sequence alignment