Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   HUS13_RS30035 Genome accession   NZ_CP054845
Coordinates   6368207..6368830 (-) Length   207 a.a.
NCBI ID   WP_003095838.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain SE5429     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6363207..6373830
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HUS13_RS30015 (HUS13_30005) aroB 6363802..6364908 (-) 1107 WP_003095831.1 3-dehydroquinate synthase -
  HUS13_RS30020 (HUS13_30010) aroK 6364958..6365476 (-) 519 WP_003095833.1 shikimate kinase AroK -
  HUS13_RS30025 (HUS13_30015) pilQ 6365488..6367632 (-) 2145 WP_003095835.1 type 4a pilus secretin PilQ Machinery gene
  HUS13_RS30030 (HUS13_30020) comL/pilP 6367686..6368210 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  HUS13_RS30035 (HUS13_30025) pilO 6368207..6368830 (-) 624 WP_003095838.1 type 4a pilus biogenesis protein PilO Machinery gene
  HUS13_RS30040 (HUS13_30030) - 6368827..6368952 (-) 126 Protein_5956 pilus assembly protein PilN -
  HUS13_RS30045 (HUS13_30035) - 6369039..6369263 (-) 225 WP_003121473.1 hypothetical protein -
  HUS13_RS30050 (HUS13_30040) - 6369337..6369624 (-) 288 WP_010791802.1 hypothetical protein -
  HUS13_RS30055 (HUS13_30045) - 6369621..6370768 (-) 1148 Protein_5959 hypothetical protein -
  HUS13_RS34220 (HUS13_30050) - 6370765..6372985 (-) 2221 Protein_5960 phage tail protein -
  HUS13_RS30065 (HUS13_30055) - 6372975..6373193 (-) 219 WP_003117240.1 hypothetical protein -
  HUS13_RS30070 (HUS13_30060) - 6373190..6373429 (-) 240 WP_003094581.1 hypothetical protein -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22805.23 Da        Isoelectric Point: 4.7787

>NTDB_id=401877 HUS13_RS30035 WP_003095838.1 6368207..6368830(-) (pilO) [Pseudomonas aeruginosa strain SE5429]
MSLASSLESLRKIDVNDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=401877 HUS13_RS30035 WP_003095838.1 6368207..6368830(-) (pilO) [Pseudomonas aeruginosa strain SE5429]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGACGTAAACGACCTCGATCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGGTACAACTTCCATCTGAGCGACA
TGCAGGCTCAGCTCGAACAGCAGGCCGCGGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTCGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTTGAGTTCGAGGAAATCAAGCTGCTTC
CCGAGGTTGCCCAGCAGTTCTACATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGGTCGCGCCCGGCAGCACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

44.211

91.787

0.406
  comO/pilO Acinetobacter baylyi ADP1

43.684

91.787

0.401