Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiA   Type   Regulator
Locus tag   GKC13_RS08795 Genome accession   NZ_CP046134
Coordinates   1712819..1714786 (-) Length   655 a.a.
NCBI ID   WP_014607872.1    Uniprot ID   Q8VTU5
Organism   Streptococcus thermophilus strain MAG_rmk202_sterm     
Function   internalize XIP (predicted from homology)   
Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
IScluster/Tn 1708528..1712781 1712819..1714786 flank 38


Gene organization within MGE regions


Location: 1708528..1714786
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GKC13_RS08765 (GKC13_08885) - 1708528..1708992 (+) 465 Protein_1738 transposase family protein -
  GKC13_RS08770 (GKC13_08890) - 1709252..1710442 (-) 1191 WP_011680651.1 cyclopropane-fatty-acyl-phospholipid synthase family protein -
  GKC13_RS08775 (GKC13_08895) - 1710524..1710850 (-) 327 WP_014607875.1 M20/M25/M40 family metallo-hydrolase -
  GKC13_RS08780 (GKC13_08900) - 1711039..1711361 (+) 323 Protein_1741 transposase -
  GKC13_RS08785 (GKC13_08905) - 1711446..1711667 (-) 222 WP_002948904.1 DUF4649 family protein -
  GKC13_RS08790 (GKC13_08910) - 1711870..1712774 (+) 905 Protein_1743 ISL3 family transposase -
  GKC13_RS08795 (GKC13_08915) amiA 1712819..1714786 (-) 1968 WP_014607872.1 peptide ABC transporter substrate-binding protein Regulator

Sequence

Protein


Download         Length: 655 a.a.        Molecular weight: 71730.84 Da        Isoelectric Point: 5.0409

>NTDB_id=401541 GKC13_RS08795 WP_014607872.1 1712819..1714786(-) (amiA) [Streptococcus thermophilus strain MAG_rmk202_sterm]
MKLKKILGITGVALLSVGMLAACSSKSSTSGTTYSNIYGSDPETLDYITSIMGGTKAVLTNGVDGLMEADKYGNLVPSVA
EDWSVSKDGLTYTYKIRKGIKWYTSEGEEYANVTAKDFVTGLKHAADAKSGALYLVQDSIAGLSDYLSGANKDFSNVGVK
AIDDYTLQYTLKKQEPYWNSKTTYGVLFPVNEDFLKNKGKDFGKSTDPTSILYNGPFLLKSLTAKSSIELTKNENYWDKK
NVHFDSIKFSYSDGSDLESIERSFSDGALSIARVFPTSSNYASVEKKYKDNIFYTEPGASTSVIGVNIDRQSYKFSAKKT
DAEKTSTKKALLNKDFRQSINFAIDRTAYQSQVNGKDGAALALRNLFVPYDFVSAGDKTFGDLVTEKMSSYGDEWSGVNF
ADGQEGLYNAEKAKTEFAKAKEALQGEGVQFPVHLDLPVDQSSKLSVAQAQSLKQTIEKSLGSENVVIDINQMSSDDMNN
VTSNAANAAAEDWDISNGVGWGPDYQDPSTYLDILKTTSSENTKIFNGYDDPNNAAAAQVGLKDYDALLDSAASETTDIN
ARYDRYAQAQAWLEDSSLIIPLTVSNGAAPVISRLTPFTGASIQVGDKGSSYVKYVKSQEKVVTKKEYEQSREKWLKERK
ASNEKAQKDLEKHVK

Nucleotide


Download         Length: 1968 bp        

>NTDB_id=401541 GKC13_RS08795 WP_014607872.1 1712819..1714786(-) (amiA) [Streptococcus thermophilus strain MAG_rmk202_sterm]
ATGAAACTTAAAAAAATTCTTGGAATTACGGGTGTAGCCCTTCTAAGTGTTGGTATGTTAGCGGCTTGTTCTTCTAAGTC
AAGTACTAGTGGAACGACTTATAGTAATATTTATGGCTCAGATCCAGAAACCTTGGATTATATTACATCAATTATGGGAG
GGACAAAAGCTGTTTTGACTAATGGTGTAGATGGTCTTATGGAAGCGGACAAATATGGTAATCTTGTTCCATCAGTAGCT
GAAGACTGGTCTGTGTCTAAAGATGGTCTGACTTATACCTATAAAATTCGTAAAGGGATCAAATGGTATACTTCTGAAGG
TGAAGAGTACGCTAATGTTACTGCTAAGGACTTTGTAACGGGTCTTAAGCATGCAGCTGATGCCAAATCTGGAGCCCTTT
ATTTGGTACAAGATTCTATTGCTGGATTGTCTGACTACCTTTCTGGTGCCAATAAAGATTTCTCAAATGTTGGCGTTAAA
GCAATAGACGATTACACTCTGCAATACACACTAAAAAAACAGGAACCATACTGGAACTCTAAGACAACCTATGGTGTGCT
CTTCCCAGTTAACGAAGACTTTCTTAAGAACAAAGGGAAAGATTTTGGTAAGTCAACAGACCCAACCTCTATCCTATATA
ACGGTCCTTTCCTTCTTAAATCTTTGACAGCTAAATCATCTATCGAGTTGACTAAGAATGAGAATTATTGGGATAAGAAA
AACGTGCATTTTGACTCTATCAAATTCTCATATTCTGATGGATCGGATCTAGAATCAATAGAACGTAGTTTCTCTGACGG
CGCTCTCAGCATTGCACGTGTCTTCCCTACGAGTTCAAACTATGCATCGGTAGAAAAAAAATATAAAGATAATATTTTTT
ACACAGAACCAGGTGCATCTACATCGGTTATTGGTGTAAACATTGACCGCCAAAGCTATAAATTCTCAGCTAAGAAGACA
GATGCTGAAAAGACATCAACTAAGAAGGCTCTTCTAAACAAAGACTTTCGTCAATCAATCAACTTCGCTATTGATCGTAC
AGCCTATCAATCACAAGTAAACGGTAAAGATGGCGCAGCTCTTGCTCTACGTAACCTTTTTGTTCCTTATGATTTTGTCA
GTGCAGGCGATAAGACCTTTGGTGATCTTGTGACAGAAAAAATGAGCTCTTATGGTGATGAGTGGTCTGGTGTTAACTTT
GCAGATGGTCAAGAGGGTCTCTACAATGCTGAAAAAGCTAAGACTGAGTTTGCCAAAGCCAAAGAGGCTCTTCAAGGAGA
AGGGGTACAATTCCCAGTTCACTTAGACCTTCCAGTTGATCAAAGTTCTAAACTTAGTGTTGCTCAAGCTCAATCTTTGA
AACAAACCATTGAAAAATCATTGGGTAGCGAAAATGTTGTTATAGATATTAACCAAATGTCATCTGATGACATGAATAAT
GTGACTAGTAATGCGGCAAATGCTGCAGCAGAAGACTGGGATATTTCTAACGGTGTGGGTTGGGGTCCAGACTATCAAGA
TCCATCAACTTATCTTGATATCTTAAAAACAACATCAAGTGAAAATACCAAGATTTTCAATGGTTATGATGATCCAAACA
ATGCAGCAGCAGCCCAAGTAGGTCTTAAGGATTATGATGCCTTGCTTGATTCAGCAGCTTCAGAAACAACAGACATCAAC
GCTCGTTACGACCGTTATGCTCAAGCCCAAGCTTGGTTGGAAGATAGCTCTCTTATCATTCCATTGACTGTTAGTAACGG
TGCAGCACCAGTTATCTCACGTTTGACACCATTTACGGGTGCTAGCATACAGGTGGGTGATAAGGGCTCATCCTACGTCA
AATATGTGAAATCACAAGAAAAAGTAGTTACTAAGAAAGAATATGAACAAAGTCGTGAAAAATGGCTTAAAGAAAGGAAA
GCATCTAACGAAAAAGCTCAAAAAGATCTTGAGAAACACGTTAAATAA

Domains

Predicted by InterproScan.

Bacterial extracellular solute-binding proteins, family 5 Middle (SBP_bac_5)

(75-520)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8VTU5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiA Streptococcus salivarius strain HSISS4

88.872

100

0.89
  amiA3 Streptococcus thermophilus LMG 18311

86.454

100

0.867
  amiA3 Streptococcus thermophilus LMD-9

86.454

100

0.867

Multiple sequence alignment