Detailed information    

insolico Bioinformatically predicted

Overview

Name   ssbA   Type   Machinery gene
Locus tag   GKC25_RS18285 Genome accession   NZ_CP046128
Coordinates   3586329..3586874 (-) Length   181 a.a.
NCBI ID   WP_034660649.1    Uniprot ID   -
Organism   Bacillus pumilus strain V17C11     
Function   ssDNA binding (predicted from homology)   
DNA processing

Genomic Context

Location: 3581329..3591874
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GKC25_RS18260 (GKC25_18060) - 3581593..3582201 (-) 609 WP_080869564.1 alpha/beta hydrolase -
  GKC25_RS18265 (GKC25_18065) - 3582198..3583169 (-) 972 WP_187704277.1 ring-cleaving dioxygenase -
  GKC25_RS18270 (GKC25_18070) - 3583361..3584878 (-) 1518 WP_034660646.1 ABC transporter permease/substrate-binding protein -
  GKC25_RS18275 (GKC25_18075) - 3584871..3585836 (-) 966 WP_034660648.1 ABC transporter ATP-binding protein -
  GKC25_RS18280 (GKC25_18080) rpsR 3586049..3586288 (-) 240 WP_008347556.1 30S ribosomal protein S18 -
  GKC25_RS18285 (GKC25_18085) ssbA 3586329..3586874 (-) 546 WP_034660649.1 single-stranded DNA-binding protein SsbA Machinery gene
  GKC25_RS18290 (GKC25_18090) rpsF 3586911..3587198 (-) 288 WP_003215073.1 30S ribosomal protein S6 -
  GKC25_RS18295 (GKC25_18095) ychF 3587307..3588407 (-) 1101 WP_034660650.1 redox-regulated ATPase YchF -
  GKC25_RS18300 (GKC25_18100) - 3588624..3588830 (-) 207 WP_342689874.1 DUF951 domain-containing protein -
  GKC25_RS18305 (GKC25_18105) - 3589125..3590444 (+) 1320 WP_034660651.1 phage hydrolase -
  GKC25_RS18310 (GKC25_18110) - 3590460..3590870 (+) 411 WP_034660652.1 hypothetical protein -

Sequence

Protein


Download         Length: 181 a.a.        Molecular weight: 19733.28 Da        Isoelectric Point: 4.6109

>NTDB_id=401412 GKC25_RS18285 WP_034660649.1 3586329..3586874(-) (ssbA) [Bacillus pumilus strain V17C11]
MLNRVVLVGRLTKDPELRYTPNGAAVATFTLAVNRTFTNQSGEREADFINCVTWRRQAENVANFLKKGSLAGVDGRLQTR
NYENQQGQRVFVTEVQAESVQFLEPKSGGAGSGGYSGGGNSGGQYYGGSQNDQNPFGNEPNQNPYGNQSNQQNRNQGNSF
NDDPFANDGKPIDISDDDLPF

Nucleotide


Download         Length: 546 bp        

>NTDB_id=401412 GKC25_RS18285 WP_034660649.1 3586329..3586874(-) (ssbA) [Bacillus pumilus strain V17C11]
ATGCTAAACCGTGTTGTATTGGTCGGAAGACTAACAAAAGACCCTGAGCTTCGCTACACGCCTAATGGTGCGGCTGTAGC
CACGTTTACTCTAGCTGTGAATCGTACGTTTACGAATCAATCTGGAGAGCGTGAAGCCGATTTCATTAACTGTGTTACTT
GGAGAAGACAAGCCGAGAACGTGGCTAATTTCTTGAAAAAAGGAAGTCTTGCCGGTGTAGACGGTCGTTTGCAGACAAGA
AACTATGAAAATCAGCAAGGACAGCGTGTCTTCGTGACAGAGGTTCAAGCTGAAAGTGTTCAATTTCTTGAGCCTAAGAG
CGGCGGTGCTGGTTCAGGTGGATACAGCGGCGGTGGCAATAGCGGAGGCCAGTATTATGGCGGTAGCCAAAACGATCAGA
ATCCATTCGGAAATGAACCAAATCAAAATCCATATGGCAATCAAAGCAACCAACAAAATCGTAATCAGGGAAATAGCTTC
AATGATGATCCATTTGCGAATGATGGTAAACCGATTGACATCTCCGATGATGATTTGCCATTCTAA

Domains

Predicted by InterproScan.

Single-strand binding protein family (SSB)

(2-103)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssbA Bacillus subtilis subsp. subtilis str. 168

84.53

100

0.845
  ssb Latilactobacillus sakei subsp. sakei 23K

55.08

100

0.569
  ssbB Bacillus subtilis subsp. subtilis str. 168

64.151

58.564

0.376

Multiple sequence alignment