Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGC   Type   Machinery gene
Locus tag   GKC25_RS10825 Genome accession   NZ_CP046128
Coordinates   2148095..2148388 (-) Length   97 a.a.
NCBI ID   WP_003217322.1    Uniprot ID   -
Organism   Bacillus pumilus strain V17C11     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2143095..2153388
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GKC25_RS10775 (GKC25_10685) sinR 2143259..2143594 (+) 336 WP_095285385.1 helix-turn-helix domain-containing protein Regulator
  GKC25_RS10780 (GKC25_10690) - 2143662..2144462 (-) 801 WP_034661283.1 TasA family protein -
  GKC25_RS10785 (GKC25_10695) - 2144520..2145092 (-) 573 WP_034662000.1 signal peptidase I -
  GKC25_RS10790 (GKC25_10700) tapA 2145144..2145671 (-) 528 WP_034661284.1 amyloid fiber anchoring/assembly protein TapA -
  GKC25_RS10795 (GKC25_10705) - 2145971..2146249 (+) 279 WP_223249903.1 DUF3889 domain-containing protein -
  GKC25_RS10800 (GKC25_10710) - 2146283..2146477 (-) 195 WP_024718846.1 YqzE family protein -
  GKC25_RS10805 (GKC25_10715) comGG 2146536..2146919 (-) 384 WP_095285386.1 competence type IV pilus minor pilin ComGG -
  GKC25_RS10810 (GKC25_10720) comGF 2146916..2147416 (-) 501 WP_095285387.1 competence type IV pilus minor pilin ComGF -
  GKC25_RS10815 (GKC25_10725) comGE 2147355..2147672 (-) 318 WP_034661291.1 competence type IV pilus minor pilin ComGE -
  GKC25_RS10820 (GKC25_10730) comGD 2147656..2148102 (-) 447 WP_095285388.1 competence type IV pilus minor pilin ComGD -
  GKC25_RS10825 (GKC25_10735) comGC 2148095..2148388 (-) 294 WP_003217322.1 competence type IV pilus major pilin ComGC Machinery gene
  GKC25_RS10830 (GKC25_10740) comGB 2148405..2149445 (-) 1041 WP_034661294.1 competence type IV pilus assembly protein ComGB -
  GKC25_RS10835 (GKC25_10745) comGA 2149426..2150443 (-) 1018 Protein_2090 competence type IV pilus ATPase ComGA -
  GKC25_RS10840 (GKC25_10750) - 2150672..2151544 (-) 873 WP_341031810.1 STAS domain-containing protein -
  GKC25_RS10850 (GKC25_10760) - 2151769..2152149 (-) 381 WP_034661298.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  GKC25_RS10855 (GKC25_10765) - 2152363..2152605 (+) 243 WP_003217440.1 DUF2626 domain-containing protein -
  GKC25_RS10860 (GKC25_10770) - 2152654..2153292 (-) 639 WP_095285390.1 MBL fold metallo-hydrolase -

Sequence

Protein


Download         Length: 97 a.a.        Molecular weight: 10739.69 Da        Isoelectric Point: 7.3837

>NTDB_id=401388 GKC25_RS10825 WP_003217322.1 2148095..2148388(-) (comGC) [Bacillus pumilus strain V17C11]
MNDKGFTLLEMLIVMLVISILLLITIPNITRHHQTIQAKGCEGLKSMVSTQVMAYELEHDGKTPSLAELEKEGYVKKGLT
CPNGKAIVIQNGHVKHE

Nucleotide


Download         Length: 294 bp        

>NTDB_id=401388 GKC25_RS10825 WP_003217322.1 2148095..2148388(-) (comGC) [Bacillus pumilus strain V17C11]
ATGAATGATAAAGGATTTACCCTGTTAGAAATGTTAATTGTCATGCTCGTGATTTCGATTCTTTTACTTATTACCATTCC
GAATATCACTCGTCACCACCAAACGATTCAAGCAAAGGGCTGTGAAGGGCTTAAATCAATGGTGAGCACACAGGTGATGG
CATATGAATTAGAGCATGATGGCAAGACACCATCTTTAGCTGAATTAGAAAAAGAAGGCTATGTCAAAAAGGGATTAACG
TGTCCTAATGGAAAAGCGATTGTCATTCAAAACGGCCATGTGAAGCATGAATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(2-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGC Bacillus subtilis subsp. subtilis str. 168

68.041

100

0.68
  comGC Staphylococcus aureus MW2

41.304

94.845

0.392
  comGC Staphylococcus aureus N315

41.304

94.845

0.392

Multiple sequence alignment