Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   HUK74_RS29280 Genome accession   NZ_CP054791
Coordinates   6189734..6190357 (-) Length   207 a.a.
NCBI ID   WP_003095838.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain SE5430     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6184734..6195357
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HUK74_RS29260 (HUK74_29220) aroB 6185329..6186435 (-) 1107 WP_003095831.1 3-dehydroquinate synthase -
  HUK74_RS29265 (HUK74_29225) aroK 6186485..6187003 (-) 519 WP_003095833.1 shikimate kinase AroK -
  HUK74_RS29270 (HUK74_29230) pilQ 6187015..6189159 (-) 2145 WP_003095835.1 type 4a pilus secretin PilQ Machinery gene
  HUK74_RS29275 (HUK74_29235) comL/pilP 6189213..6189737 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  HUK74_RS29280 (HUK74_29240) pilO 6189734..6190357 (-) 624 WP_003095838.1 type 4a pilus biogenesis protein PilO Machinery gene
  HUK74_RS29285 (HUK74_29245) pilN 6190354..6190950 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  HUK74_RS29290 (HUK74_29250) pilM 6190950..6192014 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  HUK74_RS29295 (HUK74_29255) - 6192199..6194670 (+) 2472 WP_003095841.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22805.23 Da        Isoelectric Point: 4.7787

>NTDB_id=401276 HUK74_RS29280 WP_003095838.1 6189734..6190357(-) (pilO) [Pseudomonas aeruginosa strain SE5430]
MSLASSLESLRKIDVNDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=401276 HUK74_RS29280 WP_003095838.1 6189734..6190357(-) (pilO) [Pseudomonas aeruginosa strain SE5430]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGACGTAAACGACCTCGATCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGGTACAACTTCCATCTGAGCGACA
TGCAGGCTCAGCTCGAACAGCAGGCCGCGGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTCGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTTGAGTTCGAGGAAATCAAGCTGCTTC
CCGAGGTTGCCCAGCAGTTCTACATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGGTCGCGCCCGGCAGCACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

44.211

91.787

0.406
  comO/pilO Acinetobacter baylyi ADP1

43.684

91.787

0.401

Multiple sequence alignment