Detailed information    

insolico Bioinformatically predicted

Overview

Name   recG   Type   Machinery gene
Locus tag   HT132_RS12620 Genome accession   NZ_CP054479
Coordinates   2577709..2579757 (-) Length   682 a.a.
NCBI ID   WP_014417753.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain R8-25     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2572709..2584757
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HT132_RS12590 (HT132_12590) rncS 2573113..2573862 (-) 750 WP_014417754.1 ribonuclease III -
  HT132_RS12595 (HT132_12595) acpP 2574001..2574234 (-) 234 WP_003154310.1 acyl carrier protein -
  HT132_RS12600 (HT132_12600) fabG 2574319..2575059 (-) 741 WP_003154312.1 3-oxoacyl-[acyl-carrier-protein] reductase -
  HT132_RS12605 (HT132_12605) fabD 2575052..2576005 (-) 954 WP_007409759.1 ACP S-malonyltransferase -
  HT132_RS12610 (HT132_12610) plsX 2576024..2577022 (-) 999 WP_007409758.1 phosphate acyltransferase PlsX -
  HT132_RS12615 (HT132_12615) fapR 2577036..2577602 (-) 567 WP_003154320.1 transcription factor FapR -
  HT132_RS12620 (HT132_12620) recG 2577709..2579757 (-) 2049 WP_014417753.1 ATP-dependent DNA helicase RecG Machinery gene
  HT132_RS12625 (HT132_12625) sdaAA 2579735..2580637 (-) 903 WP_174532563.1 L-serine ammonia-lyase, iron-sulfur-dependent, subunit alpha -
  HT132_RS12630 (HT132_12630) sdaAB 2580658..2581320 (-) 663 WP_017417764.1 L-serine ammonia-lyase, iron-sulfur-dependent subunit beta -
  HT132_RS12635 (HT132_12635) - 2581463..2583130 (-) 1668 WP_022553356.1 DAK2 domain-containing protein -
  HT132_RS12640 (HT132_12640) - 2583146..2583508 (-) 363 WP_003154327.1 Asp23/Gls24 family envelope stress response protein -
  HT132_RS12645 (HT132_12645) rpmB 2583767..2583955 (+) 189 WP_003154328.1 50S ribosomal protein L28 -
  HT132_RS12650 (HT132_12650) spoVM 2584033..2584113 (-) 81 WP_003154329.1 stage V sporulation protein SpoVM -

Sequence

Protein


Download         Length: 682 a.a.        Molecular weight: 77861.15 Da        Isoelectric Point: 6.7929

>NTDB_id=399899 HT132_RS12620 WP_014417753.1 2577709..2579757(-) (recG) [Bacillus amyloliquefaciens strain R8-25]
MTHHQQTSIAEIKGIGPETEKTLHELGIYDISDLLNYFPYRYDDYELRDLEEVKHEERVTVEGKVHSEPSLTYYGKKRNR
LTFRVLVGNYLITAVCFNRPYLKKKLTLGSVVTISGKWDKHRQTVSVQELKNGPHQEDKSIEPVYSVKENVTVKMMRRFI
KEALQHHLDSAADPLPEKLRIRYKLPDYKHALQTMHQPETRESLQQARRRFVYEEFLLFQLKMQAFRKAEREQSKGISHV
FPAEKLAAFTDSLPFSLTTAQTRVLREITADMTSPYRMNRLLQGDVGSGKTAVAAIALYAAILSGYQGALMVPTEILAEQ
HADSLVSLFANEDVNIALLTSSVKGKRRRELLERLALGEIDILVGTHALIQDEVEFKALSLVITDEQHRFGVEQRKKLKN
KGQDPDVLFMTATPIPRTLAITVFGEMDVSVIDEMPAGRKQIETYWVKHDMLERILAFIEKELKQGRQAYIICPLIEESD
KLDVQNAIDVYNMLSDVYRGKWNVGLMHGKLHSDEKDQVMREFSANQCQVLVSTTVVEVGVNVPNATIMVIYDADRFGLS
QLHQLRGRVGRGDHQSFCILMADPKSETGKERMRIMSETNDGFELSEKDLELRGPGDFFGKKQSGMPEFKVADMVHDYRA
LETARQDAANLVSSEAFWKDDEYRMLRGQLLSSGVLEGEKLS

Nucleotide


Download         Length: 2049 bp        

>NTDB_id=399899 HT132_RS12620 WP_014417753.1 2577709..2579757(-) (recG) [Bacillus amyloliquefaciens strain R8-25]
GTGACACACCATCAGCAAACTAGTATAGCGGAGATTAAGGGCATTGGGCCGGAAACAGAAAAAACATTGCACGAACTTGG
TATCTATGACATTTCTGATCTTCTGAATTATTTCCCTTACCGTTATGACGACTATGAGCTGAGGGATTTAGAAGAAGTAA
AACATGAAGAAAGAGTGACGGTAGAAGGGAAGGTTCATTCAGAACCTTCTCTTACCTATTACGGCAAAAAACGAAACAGG
CTGACATTCAGGGTGCTTGTCGGCAATTATTTAATTACCGCGGTCTGCTTTAACCGTCCCTACTTAAAAAAGAAACTGAC
GTTAGGTTCAGTCGTCACGATATCCGGGAAATGGGATAAACACAGACAGACCGTTTCCGTGCAGGAATTAAAAAACGGTC
CTCATCAAGAAGATAAAAGCATTGAGCCTGTTTATTCCGTTAAAGAAAACGTCACCGTAAAAATGATGAGACGGTTTATT
AAGGAAGCGCTGCAGCATCATTTGGACAGTGCGGCCGATCCGCTTCCTGAAAAATTGAGAATCCGCTACAAGCTGCCTGA
TTACAAACATGCCCTGCAGACGATGCATCAGCCTGAAACGAGGGAATCGTTACAGCAGGCAAGACGCCGGTTTGTTTACG
AGGAATTCTTATTATTTCAGCTGAAAATGCAGGCGTTCCGTAAAGCGGAAAGGGAACAGTCAAAAGGCATCAGCCATGTG
TTTCCTGCTGAAAAGCTCGCCGCTTTCACAGACAGTCTGCCGTTTTCGCTCACAACCGCACAGACGCGCGTGCTTCGGGA
AATTACCGCTGATATGACATCCCCTTACCGAATGAACCGTCTGCTGCAAGGTGATGTCGGTTCAGGGAAAACAGCCGTCG
CCGCCATCGCTTTGTACGCTGCGATTCTGTCGGGGTATCAGGGGGCATTAATGGTGCCGACTGAAATTCTGGCCGAACAG
CATGCGGATTCTCTCGTATCGTTGTTTGCAAATGAAGATGTAAATATCGCGCTTTTGACGAGTTCTGTAAAAGGAAAGCG
GCGCAGGGAGCTTTTGGAGCGGCTTGCTCTCGGAGAGATTGATATTTTAGTAGGGACCCATGCTTTAATCCAGGACGAAG
TGGAATTTAAAGCGCTGAGCCTTGTCATTACGGACGAGCAGCACAGGTTCGGAGTCGAACAGCGCAAAAAACTCAAGAAT
AAAGGTCAGGATCCGGATGTGCTGTTTATGACAGCCACCCCGATTCCGAGAACGTTGGCCATTACCGTCTTCGGAGAAAT
GGATGTTTCCGTGATAGATGAAATGCCCGCGGGGCGAAAACAAATCGAAACGTATTGGGTGAAACACGACATGCTGGAGC
GGATTTTGGCTTTTATAGAAAAAGAGCTGAAACAGGGAAGGCAAGCCTATATTATCTGCCCGCTCATTGAAGAGTCGGAT
AAACTGGATGTGCAGAATGCGATTGACGTATATAACATGCTGTCTGACGTTTACCGCGGTAAATGGAATGTCGGCCTGAT
GCACGGGAAGCTCCATTCTGATGAAAAAGATCAGGTGATGAGAGAATTCAGCGCCAATCAATGTCAGGTACTTGTGTCAA
CAACCGTCGTAGAAGTCGGGGTGAACGTGCCCAATGCGACGATTATGGTCATCTATGACGCCGACCGTTTCGGATTATCT
CAGCTCCATCAGCTCCGCGGCCGGGTCGGGCGGGGAGACCACCAATCTTTCTGCATCCTGATGGCCGATCCGAAATCCGA
AACCGGGAAGGAACGGATGAGAATCATGTCGGAGACAAACGACGGCTTTGAACTGTCTGAAAAGGACCTTGAACTGCGCG
GCCCCGGTGATTTCTTCGGGAAAAAACAGAGCGGTATGCCCGAGTTCAAGGTGGCGGATATGGTTCATGACTACAGAGCT
TTGGAAACGGCCCGCCAGGATGCGGCCAATCTGGTGTCTTCAGAGGCTTTTTGGAAAGATGACGAATACCGTATGCTGCG
CGGCCAATTACTTTCAAGCGGCGTCCTAGAAGGGGAGAAATTAAGCTGA

Domains

Predicted by InterProScan.

DEAD/DEAH box helicase (DEAD)

(261-415)

Helicase conserved C-terminal domain (Helicase_C)

(452-572)

RecG wedge domain (RecG_wedge)

(11-164)

ATP-dependent DNA helicase RecG, domain 3, C-terminal (RecG_dom3_C)

(599-670)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recG Bacillus subtilis subsp. subtilis str. 168

89.736

100

0.897
  recG/mmsA Streptococcus pneumoniae R6

50.075

97.801

0.49
  recG/mmsA Streptococcus pneumoniae R36A

50.075

97.801

0.49
  recG Neisseria meningitidis strain C311

39.357

95.748

0.377