Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   HPE41_RS18665 Genome accession   NZ_CP054371
Coordinates   3973947..3974606 (+) Length   219 a.a.
NCBI ID   WP_001221502.1    Uniprot ID   B7LGG8
Organism   Escherichia coli strain SCU-316     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 3968947..3979606
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HPE41_RS18650 (HPE41_18650) ygiS 3971152..3972759 (-) 1608 WP_001308070.1 ABC transporter substrate-binding protein -
  HPE41_RS18655 (HPE41_18655) ygiV 3972868..3973350 (-) 483 WP_000183494.1 GyrI-like domain-containing protein -
  HPE41_RS18660 (HPE41_18660) ygiW 3973403..3973795 (-) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  HPE41_RS18665 (HPE41_18665) ciaR 3973947..3974606 (+) 660 WP_001221502.1 quorum sensing response regulator transcription factor QseB Regulator
  HPE41_RS18670 (HPE41_18670) qseC 3974603..3975952 (+) 1350 WP_000673348.1 quorum sensing histidine kinase QseC -
  HPE41_RS18675 (HPE41_18675) ygiZ 3975998..3976330 (-) 333 WP_000912091.1 DUF2645 family protein -
  HPE41_RS18680 (HPE41_18680) mdaB 3976688..3977269 (+) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  HPE41_RS18685 (HPE41_18685) ygiN 3977300..3977614 (+) 315 WP_000958598.1 putative quinol monooxygenase -
  HPE41_RS18690 (HPE41_18690) parE 3977662..3979554 (-) 1893 WP_000195296.1 DNA topoisomerase IV subunit B -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24745.66 Da        Isoelectric Point: 6.6543

>NTDB_id=399233 HPE41_RS18665 WP_001221502.1 3973947..3974606(+) (ciaR) [Escherichia coli strain SCU-316]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALEERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=399233 HPE41_RS18665 WP_001221502.1 3973947..3974606(+) (ciaR) [Escherichia coli strain SCU-316]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGAGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAACTGCGCCACGGGAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTG
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LGG8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365