Detailed information NTG0039920

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: DEIPR_RS06640
• Genome accession: NC_015161
• Coordinates: 1394123..1396765 (-)
• Length: 880 a.a.
• NCBI ID: WP_013615072.1
• Uniprot ID: F0RPC0
• Organism: Deinococcus proteolyticus MRP
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1389123..1401765

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DEIPR_RS06630 (Deipr_1312)	lon	1390286..1392775 (+)	2490	WP_013615070.1	endopeptidase La	-
DEIPR_RS06635 (Deipr_1313)	pilT	1392885..1394090 (-)	1206	WP_013615071.1	type IV pilus twitching motility protein PilT	Machinery gene
DEIPR_RS06640 (Deipr_1314)	pilB	1394123..1396765 (-)	2643	WP_013615072.1	type II/IV secretion system protein	Machinery gene
DEIPR_RS06645 (Deipr_1315)	-	1396841..1397383 (-)	543	WP_049775247.1	YqeG family HAD IIIA-type phosphatase	-
DEIPR_RS06650 (Deipr_1316)	-	1397389..1398147 (-)	759	WP_013615074.1	polyphenol oxidase family protein	-
DEIPR_RS06655 (Deipr_1317)	-	1398286..1399083 (+)	798	WP_013615075.1	enoyl-ACP reductase	-
DEIPR_RS06660 (Deipr_1318)	-	1399181..1399672 (+)	492	WP_013615076.1	hypothetical protein	-
DEIPR_RS06665 (Deipr_1319)	-	1399720..1400787 (+)	1068	WP_013615077.1	M42 family metallopeptidase	-
DEIPR_RS06670 (Deipr_1320)	-	1400943..1401197 (+)	255	WP_013615078.1	hypothetical protein	-

Sequence

Protein

• Length: 880 a.a.
• Molecular weight: 96174.87 Da
• Isoelectric Point: 4.6675

>NTDB_id=39920 DEIPR_RS06640 WP_013615072.1 1394123..1396765(-) (pilB) [Deinococcus proteolyticus MRP]
MTTLSIADRKLGAVLLDGGYITDEDLQRMLVRHAEVGGRLADVMIDSGVVGEKRLAHAIESSLGIPAIDLMAVEPQPQAL
ATIPGHVAQNAAAVPFALEGDLLKVAMLDPLSTLTIEVLEDETGYDIEPYQAMRSALNWRLAIHYPELGLTPQRPDQAAP
GQERLGDYLVRRSIITPQQLEAGLKFQERTGEPLGQVLIGQGALNDEQLYRALANQQGLEFLPDLSTFTVPERVLEVLSR
TDAVRYGALPLGQQEEGYEVVGYDPRYFAQVQEQIGQPVQFLMSLPTQVEKAIERFYPAPAPAPFRDEMQSLYAGSGGQA
KSLTDVIMEMGFADSEDIQAAMQQQQQGGARLEDTLVQSGKLSPEMLAQSLATQLGYEFIDPAKNPPDSGVSMLVPEATA
RRYSVVPVRLEGEALVVAMKDPRNVFALDDLRLITGREIVPAVMVEKEITRLIEKYFGSKDMAELNQRLLEESRERQKLQ
ESAGAGDMDTALDDNAVVRTVENLIREAVLQDVSDIHIEPTETELLVRYRIDGALRKHASLPKGSAQALLARIKIMGGLD
IAERRVPQDGRVRFRKGAIDIDLRLSTLPTVYGEKAVMRLLQKAENIPEVEQLGFSDHNFRRYLEVIDKPNGIFLVTGPT
GSGKSFTSFSTLKRIAKPEKNTTTIEDPVEYEIPGIMQSQVNNAAGMTFAKALRAFLRQDPDIIFVGEIRDGETAKIATE
AALTGHLVLATLHTNDAAGAIVRLEEMGVELFNIGAAVIGVLAQRLVRRVCKDCAQPTAADPEVLRRLGLSDADIQGATL
MKGAGCPRCGGTGYKGRTGIHELLVIDDTIRRAINSGKTATDIKDIAIEEGDMWTLRTDGIHKALQGITTLEEVLAVTSN

Nucleotide

• Length: 2643 bp

>NTDB_id=39920 DEIPR_RS06640 WP_013615072.1 1394123..1396765(-) (pilB) [Deinococcus proteolyticus MRP]
ATGACGACTCTATCTATCGCTGACCGTAAGCTGGGCGCCGTACTGCTGGACGGCGGCTACATCACCGACGAGGACCTGCA
GCGCATGTTGGTGCGCCACGCTGAGGTGGGCGGGCGCCTGGCCGACGTGATGATCGACTCGGGTGTGGTGGGCGAAAAGC
GCCTGGCCCACGCCATCGAGTCCAGCCTGGGCATTCCGGCCATCGACCTGATGGCGGTGGAGCCGCAGCCCCAGGCGCTG
GCGACCATTCCCGGCCACGTGGCGCAGAACGCCGCCGCCGTGCCGTTCGCCCTGGAAGGCGACCTGCTGAAAGTCGCCAT
GCTCGACCCGCTGTCCACCCTCACCATCGAGGTGCTGGAAGACGAAACCGGCTACGACATCGAGCCGTATCAGGCCATGC
GCTCGGCACTGAACTGGCGGCTGGCGATCCACTACCCCGAACTGGGCCTGACGCCGCAGCGCCCCGACCAGGCCGCCCCC
GGCCAGGAGCGTCTGGGCGACTATCTGGTGCGCCGGAGCATCATCACGCCCCAGCAGTTGGAAGCCGGCCTGAAGTTTCA
GGAGCGTACCGGCGAGCCGCTGGGTCAGGTGCTGATCGGTCAAGGAGCGCTGAACGACGAGCAGCTGTACCGCGCCCTGG
CCAACCAGCAGGGGCTGGAATTCCTGCCCGACCTGAGCACGTTCACGGTGCCGGAGCGGGTGCTGGAAGTGCTGTCGCGC
ACCGACGCCGTGCGCTACGGCGCCCTGCCGCTGGGTCAGCAGGAAGAAGGCTACGAGGTGGTGGGGTACGACCCACGTTA
CTTCGCGCAGGTGCAGGAGCAGATTGGGCAGCCGGTGCAGTTCCTGATGAGCTTGCCCACCCAGGTGGAAAAGGCCATCG
AGCGCTTTTACCCGGCGCCGGCTCCTGCGCCCTTCCGCGACGAGATGCAGTCGCTGTACGCCGGCAGCGGTGGTCAGGCC
AAGTCGCTGACCGACGTGATTATGGAAATGGGCTTTGCCGATTCCGAGGATATTCAGGCGGCCATGCAGCAGCAGCAGCA
GGGCGGCGCCCGGCTGGAAGACACCCTGGTGCAGTCGGGCAAGCTGAGCCCCGAAATGCTGGCACAGTCGCTGGCGACCC
AGCTGGGCTACGAGTTCATCGACCCGGCCAAGAACCCGCCCGACAGCGGCGTGTCCATGCTGGTGCCGGAAGCCACCGCC
CGGCGCTACAGCGTGGTGCCGGTGCGCCTGGAAGGCGAGGCGCTGGTGGTCGCCATGAAAGACCCGCGCAACGTGTTCGC
GCTGGACGACCTGCGCCTGATCACCGGCCGCGAGATTGTCCCCGCCGTGATGGTGGAAAAGGAAATCACCCGCCTGATCG
AGAAGTATTTCGGCTCCAAGGACATGGCGGAGCTGAACCAGCGGCTGCTGGAAGAAAGCCGCGAGCGCCAGAAGTTGCAG
GAGTCGGCCGGGGCCGGCGATATGGACACCGCGCTGGACGACAACGCCGTGGTCCGCACCGTGGAAAACCTGATTCGCGA
AGCTGTGCTGCAGGACGTGAGTGATATTCACATTGAGCCCACCGAAACCGAGCTGCTGGTGCGTTACCGCATCGACGGGG
CACTGCGCAAGCACGCCTCTTTGCCCAAGGGTTCGGCGCAGGCACTGCTGGCCCGTATCAAAATCATGGGCGGCCTCGAC
ATCGCCGAGCGCCGGGTGCCGCAAGACGGGCGCGTGCGCTTCCGCAAGGGCGCCATCGACATCGACCTGCGTCTGTCCAC
CCTGCCCACCGTATACGGCGAGAAGGCCGTGATGCGTCTGCTGCAAAAGGCCGAGAACATCCCCGAGGTGGAGCAGCTGG
GCTTCAGCGACCACAACTTCCGCCGCTACCTGGAAGTGATTGACAAGCCCAACGGCATCTTTTTGGTCACTGGCCCGACG
GGTTCGGGCAAGTCGTTCACCAGCTTTTCCACCCTCAAGCGCATCGCCAAGCCGGAGAAGAACACCACCACCATCGAAGA
CCCGGTAGAATACGAAATCCCCGGCATCATGCAGAGCCAGGTGAACAACGCGGCGGGCATGACCTTCGCCAAGGCGCTGC
GGGCCTTCCTCCGTCAGGACCCCGACATCATCTTCGTGGGGGAAATCCGTGACGGTGAAACGGCCAAAATCGCCACCGAA
GCGGCGCTGACCGGTCACTTGGTGCTGGCGACCCTCCACACCAACGACGCGGCCGGCGCGATTGTGCGCCTGGAGGAAAT
GGGCGTGGAGCTGTTCAACATCGGCGCGGCGGTGATTGGCGTGCTGGCCCAGCGCCTGGTGCGCCGGGTCTGCAAGGACT
GTGCCCAGCCCACGGCCGCCGACCCCGAGGTGCTGCGCCGCCTCGGCCTGAGCGACGCCGACATTCAGGGCGCCACCCTG
ATGAAGGGTGCCGGCTGCCCGCGCTGCGGCGGCACCGGCTACAAAGGCCGCACCGGCATCCACGAGCTGCTGGTGATTGA
TGACACCATCCGCCGGGCCATCAACAGCGGCAAAACGGCCACCGACATCAAGGACATCGCCATCGAGGAAGGGGACATGT
GGACCCTGCGCACCGACGGCATCCACAAGGCCCTGCAGGGCATCACCACCCTGGAAGAAGTGCTGGCCGTGACCAGCAAC
TGA

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (45-146)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (198-301)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (356-460)

• Type II/IV secretion system protein (T2SSE) (504-769)

3D structure

• AlphaFold DB: F0RPC0

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	63.3	100	0.641
pilF	Thermus thermophilus HB27	56.013	100	0.572