Detailed information    

insolico Bioinformatically predicted

Overview


Name   phrF   Type   Regulator
Locus tag   GII80_RS20035 Genome accession   NZ_CP045821
Coordinates   3822802..3822921 (+) Length   39 a.a.
NCBI ID   WP_009968329.1    Uniprot ID   A0A199WEM2
Organism   Bacillus subtilis strain MB8_B7     
Function   antagonize RapF (predicted from homology)   
Competence regulation

Genomic Context


Location: 3817802..3827921
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GII80_RS20010 (GII80_20010) albG 3817966..3818667 (+) 702 WP_003243391.1 antilisterial bacteriocin subtilosin biosynthesis protein AlbG -
  GII80_RS20015 (GII80_20015) ywhL 3818673..3820049 (-) 1377 WP_003244415.1 YncE family protein -
  GII80_RS20020 (GII80_20020) ywhK 3820088..3821443 (-) 1356 WP_003227552.1 YncE family protein -
  GII80_RS22735 (GII80_20025) - 3821440..3821670 (-) 231 WP_009968328.1 hypothetical protein -
  GII80_RS20030 (GII80_20030) rapF 3821673..3822818 (+) 1146 WP_003227549.1 response regulator aspartate phosphatase RapF Regulator
  GII80_RS20035 (GII80_20035) phrF 3822802..3822921 (+) 120 WP_009968329.1 phosphatase RapF inhibitor PhrF Regulator
  GII80_RS20040 (GII80_20040) ywhH 3823020..3823493 (+) 474 WP_003227547.1 YbaK/EbsC family protein -
  GII80_RS20045 (GII80_20045) speB 3823525..3824397 (-) 873 WP_003227545.1 agmatinase -
  GII80_RS20050 (GII80_20050) speE 3824458..3825288 (-) 831 WP_003227543.1 spermidine synthase -
  GII80_RS20055 (GII80_20055) pbpG 3825490..3827565 (+) 2076 WP_003227540.1 transglycosylase domain-containing protein -

Sequence


Protein


Download         Length: 39 a.a.        Molecular weight: 4199.10 Da        Isoelectric Point: 10.4997

>NTDB_id=398101 GII80_RS20035 WP_009968329.1 3822802..3822921(+) (phrF) [Bacillus subtilis strain MB8_B7]
MKLKSKLLLSCLALSTVFVATTIANAPTHQIEVAQRGMI

Nucleotide


Download         Length: 120 bp        

>NTDB_id=398101 GII80_RS20035 WP_009968329.1 3822802..3822921(+) (phrF) [Bacillus subtilis strain MB8_B7]
ATGAAATTGAAGTCTAAACTATTACTCTCTTGTCTGGCTCTAAGCACTGTGTTCGTGGCAACAACTATTGCAAATGCACC
TACACACCAAATTGAAGTTGCACAACGAGGAATGATTTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A199WEM2

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  phrF Bacillus subtilis subsp. subtilis str. 168

100

100

1

  phrC Bacillus subtilis subsp. subtilis str. 168

48.718

100

0.487


Multiple sequence alignment