Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   HRL54_RS03375 Genome accession   NZ_CP054191
Coordinates   694597..695256 (-) Length   219 a.a.
NCBI ID   WP_001221502.1    Uniprot ID   B7LGG8
Organism   Escherichia coli strain TH9F11     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 689597..700256
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HRL54_RS03350 (HRL54_03315) parE 689688..691580 (+) 1893 WP_000195292.1 DNA topoisomerase IV subunit B -
  HRL54_RS03355 (HRL54_03320) ygiN 691628..691942 (-) 315 WP_000958598.1 putative quinol monooxygenase -
  HRL54_RS03360 (HRL54_03325) mdaB 691973..692554 (-) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  HRL54_RS03365 (HRL54_03330) ygiZ 692873..693205 (+) 333 WP_000912120.1 DUF2645 family protein -
  HRL54_RS03370 (HRL54_03335) qseC 693251..694600 (-) 1350 WP_000673354.1 quorum sensing histidine kinase QseC -
  HRL54_RS03375 (HRL54_03340) ciaR 694597..695256 (-) 660 WP_001221502.1 quorum sensing response regulator transcription factor QseB Regulator
  HRL54_RS03380 (HRL54_03345) ygiW 695408..695800 (+) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  HRL54_RS03385 (HRL54_03350) ygiV 695853..696335 (+) 483 WP_000183494.1 AraC family transcriptional regulator -
  HRL54_RS03390 (HRL54_03355) parC 696881..699139 (+) 2259 WP_001281841.1 DNA topoisomerase IV subunit A -
  HRL54_RS03395 (HRL54_03360) plsC 699373..700110 (+) 738 WP_000965712.1 1-acylglycerol-3-phosphate O-acyltransferase -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24745.66 Da        Isoelectric Point: 6.6543

>NTDB_id=397915 HRL54_RS03375 WP_001221502.1 694597..695256(-) (ciaR) [Escherichia coli strain TH9F11]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALEERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=397915 HRL54_RS03375 WP_001221502.1 694597..695256(-) (ciaR) [Escherichia coli strain TH9F11]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCGCCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGAGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LGG8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365