Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   GII82_RS12850 Genome accession   NZ_CP045819
Coordinates   2509980..2510597 (-) Length   205 a.a.
NCBI ID   WP_004398514.1    Uniprot ID   A0AAE2SLR2
Organism   Bacillus subtilis strain MB9_B4     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2504980..2515597
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GII82_RS12820 (GII82_12820) rpsT 2505282..2505548 (+) 267 WP_003229989.1 30S ribosomal protein S20 -
  GII82_RS12825 (GII82_12825) holA 2505563..2506606 (-) 1044 WP_003229987.1 DNA polymerase III subunit delta -
  GII82_RS12830 (GII82_12830) - 2506646..2506795 (-) 150 WP_003229985.1 hypothetical protein -
  GII82_RS12835 (GII82_12835) yqzM 2506836..2506970 (+) 135 WP_003229983.1 YqzM family protein -
  GII82_RS12840 (GII82_12840) comEC 2507010..2509340 (-) 2331 WP_009967776.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  GII82_RS12845 (GII82_12845) comEB 2509344..2509913 (-) 570 WP_003229978.1 ComE operon protein 2 -
  GII82_RS12850 (GII82_12850) comEA 2509980..2510597 (-) 618 WP_004398514.1 competence protein ComEA Machinery gene
  GII82_RS12855 (GII82_12855) comER 2510681..2511502 (+) 822 WP_004398597.1 late competence protein ComER -
  GII82_RS12860 (GII82_12860) yqeM 2511568..2512311 (-) 744 WP_003229973.1 class I SAM-dependent methyltransferase -
  GII82_RS12865 (GII82_12865) rsfS 2512308..2512664 (-) 357 WP_003229971.1 ribosome silencing factor -
  GII82_RS12870 (GII82_12870) yqeK 2512682..2513242 (-) 561 WP_004399059.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  GII82_RS12875 (GII82_12875) nadD 2513232..2513801 (-) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  GII82_RS12880 (GII82_12880) yhbY 2513813..2514103 (-) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  GII82_RS12885 (GII82_12885) aroE 2514097..2514939 (-) 843 WP_003229967.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21769.45 Da        Isoelectric Point: 4.7220

>NTDB_id=397911 GII82_RS12850 WP_004398514.1 2509980..2510597(-) (comEA) [Bacillus subtilis strain MB9_B4]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=397911 GII82_RS12850 WP_004398514.1 2509980..2510597(-) (comEA) [Bacillus subtilis strain MB9_B4]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAGGAAAACGGTCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(142-203)

SLBB domain (SLBB)

(63-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

100

100

1
  comEA Staphylococcus aureus MW2

37.273

100

0.4
  comEA Staphylococcus aureus N315

36.364

100

0.39

Multiple sequence alignment