Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   BSN5_RS03335 Genome accession   NC_014976
Coordinates   618605..619222 (-) Length   205 a.a.
NCBI ID   WP_004398514.1    Uniprot ID   A0AAE2SLR2
Organism   Bacillus subtilis BSn5     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 613605..624222
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BSN5_RS03310 (BSn5_03335) rpsT 613914..614180 (+) 267 WP_003229989.1 30S ribosomal protein S20 -
  BSN5_RS03315 (BSn5_03340) holA 614195..615238 (-) 1044 WP_015714286.1 DNA polymerase III subunit delta -
  BSN5_RS21840 - 615278..615427 (-) 150 WP_003229985.1 hypothetical protein -
  BSN5_RS03320 (BSn5_03345) yqzM 615468..615602 (+) 135 WP_003229983.1 YqzM family protein -
  BSN5_RS03325 (BSn5_03350) comEC 615642..617972 (-) 2331 WP_074794436.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  BSN5_RS03330 (BSn5_03355) comEB 617969..618538 (-) 570 WP_015714288.1 ComE operon protein 2 -
  BSN5_RS03335 (BSn5_03360) comEA 618605..619222 (-) 618 WP_004398514.1 competence protein ComEA Machinery gene
  BSN5_RS03340 (BSn5_03365) comER 619306..620127 (+) 822 WP_015714289.1 late competence protein ComER -
  BSN5_RS03345 (BSn5_03370) yqeM 620193..620936 (-) 744 WP_015714290.1 class I SAM-dependent methyltransferase -
  BSN5_RS03350 (BSn5_03375) rsfS 620933..621289 (-) 357 WP_014480315.1 ribosome silencing factor -
  BSN5_RS03355 (BSn5_03380) yqeK 621307..621867 (-) 561 WP_004399059.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  BSN5_RS03360 (BSn5_03385) nadD 621857..622426 (-) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  BSN5_RS03365 (BSn5_03390) yhbY 622438..622728 (-) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  BSN5_RS03370 (BSn5_03395) aroE 622722..623564 (-) 843 WP_015714291.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21769.45 Da        Isoelectric Point: 4.7220

>NTDB_id=39749 BSN5_RS03335 WP_004398514.1 618605..619222(-) (comEA) [Bacillus subtilis BSn5]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=39749 BSN5_RS03335 WP_004398514.1 618605..619222(-) (comEA) [Bacillus subtilis BSn5]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGTCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(142-203)

SLBB domain (SLBB)

(63-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

100

100

1
  comEA Staphylococcus aureus MW2

37.273

100

0.4
  comEA Staphylococcus aureus N315

36.364

100

0.39

Multiple sequence alignment