Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilW   Type   Machinery gene
Locus tag   TSC_RS10145 Genome accession   NC_014974
Coordinates   1961372..1962316 (+) Length   314 a.a.
NCBI ID   WP_015717937.1    Uniprot ID   E8PND9
Organism   Thermus scotoductus SA-01     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1956372..1967316
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TSC_RS10120 (TSC_c20640) - 1957247..1957756 (+) 510 WP_041439009.1 DUF5317 domain-containing protein -
  TSC_RS10125 (TSC_c20650) - 1957829..1958833 (+) 1005 WP_015717933.1 homoisocitrate dehydrogenase -
  TSC_RS10130 (TSC_c20660) pilM 1959013..1960146 (+) 1134 WP_015717934.1 type IV pilus assembly protein PilM Machinery gene
  TSC_RS10135 (TSC_c20670) pilN 1960139..1960762 (+) 624 WP_015717935.1 competence protein Machinery gene
  TSC_RS10140 (TSC_c20680) pilO 1960752..1961375 (+) 624 WP_015717936.1 type 4a pilus biogenesis protein PilO Machinery gene
  TSC_RS10145 (TSC_c20690) pilW 1961372..1962316 (+) 945 WP_015717937.1 hypothetical protein Machinery gene
  TSC_RS10150 (TSC_c20700) pilQ 1962313..1964592 (+) 2280 WP_041438673.1 secretin N-terminal domain-containing protein Machinery gene
  TSC_RS10155 (TSC_c20710) aroC 1964696..1965847 (+) 1152 WP_015717939.1 chorismate synthase -
  TSC_RS10160 (TSC_c20720) - 1965865..1966419 (+) 555 WP_015717940.1 shikimate kinase -

Sequence

Protein


Download         Length: 314 a.a.        Molecular weight: 32421.78 Da        Isoelectric Point: 6.4954

>NTDB_id=39726 TSC_RS10145 WP_015717937.1 1961372..1962316(+) (pilW) [Thermus scotoductus SA-01]
MKALLARLATAWRNLPQSTKLLLAGLLLVSAVALWYVGFYLPAQAPMEVQPTPGQPQVPSQGAEAPKAIEAPPIPPLAET
APQDQAAKPSPEASPSQKPVLTTALPIPKTQQEAPLPNPFVPLVVEAPPSPPVPSPAPAPRPTPVPTGAPVRVTQGTPLP
TPSVQASPRPLPGSQGALPAPKVLTPAFQVETPKAQVDTPLLLTPPAGLVEAPLPRAPQTPEGGKTEPSPATSPKTPLQA
LVEEKGIKLAGTLLGPVSVAILETKEGYLVLPVGSLLPGSEAVLRRIESDRVVLALKDESLEITLEKMQAGGGQ

Nucleotide


Download         Length: 945 bp        

>NTDB_id=39726 TSC_RS10145 WP_015717937.1 1961372..1962316(+) (pilW) [Thermus scotoductus SA-01]
GTGAAAGCCCTTCTAGCGCGCCTGGCCACGGCTTGGCGCAACCTACCCCAGTCCACCAAGCTCCTCCTGGCGGGCCTTCT
CCTGGTGAGCGCCGTGGCGCTTTGGTATGTGGGCTTCTACCTGCCCGCCCAGGCACCCATGGAGGTGCAACCCACCCCCG
GCCAACCTCAGGTGCCCAGCCAGGGAGCGGAGGCTCCCAAGGCCATAGAAGCTCCACCCATCCCGCCCCTTGCCGAAACT
GCCCCCCAAGACCAAGCGGCGAAGCCTAGCCCCGAGGCTTCTCCTTCCCAGAAGCCCGTCTTGACCACAGCCTTGCCCAT
CCCCAAAACGCAACAAGAGGCCCCGCTTCCAAACCCCTTTGTGCCCCTGGTGGTGGAAGCTCCACCTTCTCCCCCCGTGC
CCTCCCCTGCCCCGGCACCTAGGCCCACCCCCGTACCCACAGGGGCCCCTGTCCGGGTAACCCAGGGAACACCCTTGCCC
ACCCCAAGCGTTCAAGCTTCACCCCGGCCTCTCCCGGGAAGCCAGGGAGCCCTGCCCGCTCCCAAGGTGCTTACCCCTGC
TTTCCAGGTGGAAACCCCCAAAGCCCAGGTGGACACTCCACTTCTCCTTACCCCACCCGCTGGCCTTGTGGAGGCTCCTT
TACCCAGGGCACCCCAAACCCCAGAGGGAGGAAAGACCGAACCTTCCCCTGCCACTTCCCCCAAAACCCCCTTGCAAGCC
CTGGTGGAGGAGAAAGGCATCAAGCTGGCGGGTACGCTTTTGGGCCCGGTGAGCGTGGCTATACTGGAAACCAAGGAAGG
GTATCTGGTATTGCCCGTGGGCAGCCTTCTTCCAGGTAGCGAGGCAGTGCTTCGCCGGATAGAAAGTGACCGGGTGGTGT
TGGCCTTGAAGGATGAAAGCTTGGAGATTACTTTAGAAAAGATGCAAGCAGGAGGTGGTCAGTGA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB E8PND9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilW Thermus thermophilus HB27

51.887

100

0.525

Multiple sequence alignment