Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   TSC_RS10130 Genome accession   NC_014974
Coordinates   1959013..1960146 (+) Length   377 a.a.
NCBI ID   WP_015717934.1    Uniprot ID   E8PND6
Organism   Thermus scotoductus SA-01     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1954013..1965146
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TSC_RS13345 (TSC_c20600) - 1954533..1954898 (-) 366 WP_015717930.1 hypothetical protein -
  TSC_RS10115 (TSC_c20630) - 1955796..1957211 (+) 1416 WP_015717931.1 HD-GYP domain-containing protein -
  TSC_RS10120 (TSC_c20640) - 1957247..1957756 (+) 510 WP_041439009.1 DUF5317 domain-containing protein -
  TSC_RS10125 (TSC_c20650) - 1957829..1958833 (+) 1005 WP_015717933.1 homoisocitrate dehydrogenase -
  TSC_RS10130 (TSC_c20660) pilM 1959013..1960146 (+) 1134 WP_015717934.1 type IV pilus assembly protein PilM Machinery gene
  TSC_RS10135 (TSC_c20670) pilN 1960139..1960762 (+) 624 WP_015717935.1 competence protein Machinery gene
  TSC_RS10140 (TSC_c20680) pilO 1960752..1961375 (+) 624 WP_015717936.1 type 4a pilus biogenesis protein PilO Machinery gene
  TSC_RS10145 (TSC_c20690) pilW 1961372..1962316 (+) 945 WP_015717937.1 hypothetical protein Machinery gene
  TSC_RS10150 (TSC_c20700) pilQ 1962313..1964592 (+) 2280 WP_041438673.1 secretin N-terminal domain-containing protein Machinery gene

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 41225.78 Da        Isoelectric Point: 4.4107

>NTDB_id=39723 TSC_RS10130 WP_015717934.1 1959013..1960146(+) (pilM) [Thermus scotoductus SA-01]
MFSGFSKLFKPRVEALGLEIGAANLKLVELSGNPPALRALATRPTPPGMLVEGVIAEPQALAQELKELLAEARTKKRYVV
TAVPNPSVILRTLQVPKMPLKEMEEAVRWEAERYIPFPIDEVVLDFAPLDPLAEVADGEQVEVMVGAARQEAVASLLEAL
RGAGLTPIILDVKPFAGLYPLEAQLSSDPEGVSVAVEIGAESTSLVLLKGDRPLAVRILTLSGKDFTEAIGKSFGLDFLT
AEEVKRTYGLATIPTEDEELLLDFDAERERYSPAKIYDAIRPILVELTQEIRRSLEFFRVQLGDIQPEVGYLYGGGSRLR
GLSTLLTDTLGVNFTVPDPWQGIQVDPKRFDLDKIKEMGPEFLVPVGLALRGVMPLD

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=39723 TSC_RS10130 WP_015717934.1 1959013..1960146(+) (pilM) [Thermus scotoductus SA-01]
GTGTTTTCGGGTTTTAGCAAGCTATTCAAACCCCGCGTGGAGGCGCTGGGCCTGGAGATCGGCGCCGCCAACCTAAAGCT
GGTGGAGCTCTCCGGAAATCCCCCCGCCCTCAGAGCCCTGGCCACCCGGCCCACTCCCCCAGGAATGCTGGTGGAAGGGG
TGATCGCCGAACCCCAGGCCCTGGCCCAGGAACTCAAGGAACTTCTCGCCGAAGCCCGGACCAAAAAGCGCTACGTGGTC
ACCGCCGTGCCCAATCCCAGCGTGATCCTGCGCACCCTTCAGGTGCCCAAGATGCCCCTCAAGGAGATGGAGGAGGCGGT
TCGCTGGGAAGCGGAGCGGTACATCCCCTTCCCCATTGACGAGGTGGTCCTGGACTTCGCCCCCCTGGATCCCCTGGCCG
AGGTAGCCGATGGGGAGCAGGTGGAGGTCATGGTGGGGGCCGCCCGGCAGGAAGCAGTGGCCTCCTTGCTGGAAGCCCTG
CGGGGAGCAGGTCTGACCCCCATCATCTTGGATGTGAAGCCCTTTGCCGGGCTTTATCCCCTGGAAGCCCAGCTCAGCAG
CGACCCGGAAGGGGTTTCCGTGGCCGTAGAAATCGGGGCAGAAAGCACCAGCCTGGTTCTCCTTAAGGGAGACCGTCCCT
TGGCGGTGCGGATCCTCACCCTCTCCGGCAAGGACTTCACCGAAGCCATCGGGAAAAGCTTTGGCCTGGACTTCCTCACC
GCCGAGGAGGTGAAGCGCACCTACGGCCTCGCCACCATCCCCACCGAGGACGAGGAGCTTCTTCTGGACTTTGACGCCGA
GAGGGAGCGGTACAGCCCCGCCAAAATCTACGATGCCATCCGGCCCATCCTGGTGGAACTCACTCAGGAGATCCGTCGGA
GCCTGGAGTTTTTCCGCGTGCAACTGGGTGACATACAGCCGGAGGTAGGGTACCTCTACGGCGGGGGAAGCCGGCTTAGG
GGCCTGTCCACCCTGCTTACCGATACCCTGGGGGTCAACTTCACCGTCCCCGACCCCTGGCAGGGCATCCAGGTGGATCC
CAAGCGCTTTGACCTGGACAAGATCAAGGAGATGGGACCGGAGTTTCTGGTGCCCGTGGGCCTGGCCTTACGGGGGGTGA
TGCCCCTTGATTAG

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(17-372)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB E8PND6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Thermus thermophilus HB27

76.393

100

0.764

Multiple sequence alignment