Detailed information NTG0397247

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: GH799_RS06150
• Genome accession: NZ_CP045794
• Coordinates: 1205573..1206442 (-)
• Length: 289 a.a.
• NCBI ID: WP_005493974.1
• Uniprot ID: A0A249W3M2
• Organism: Vibrio parahaemolyticus 10329
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1200573..1211442

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GH799_RS06115 (GH799_06110)	rpsP	1201534..1201782 (+)	249	WP_005379962.1	30S ribosomal protein S16	-
GH799_RS06120 (GH799_06115)	rimM	1201811..1202359 (+)	549	WP_005462552.1	ribosome maturation factor RimM	-
GH799_RS06125 (GH799_06120)	trmD	1202387..1203130 (+)	744	WP_005462562.1	tRNA (guanosine(37)-N1)-methyltransferase TrmD	-
GH799_RS06130 (GH799_06125)	rplS	1203172..1203525 (+)	354	WP_005462554.1	50S ribosomal protein L19	-
GH799_RS06135 (GH799_06130)	yacG	1203927..1204121 (-)	195	WP_005462546.1	DNA gyrase inhibitor YacG	-
GH799_RS06140 (GH799_06135)	zapD	1204190..1204930 (-)	741	WP_005480890.1	cell division protein ZapD	-
GH799_RS06145 (GH799_06140)	coaE	1204958..1205572 (-)	615	WP_005493969.1	dephospho-CoA kinase	-
GH799_RS06150 (GH799_06145)	pilD	1205573..1206442 (-)	870	WP_005493974.1	A24 family peptidase	Machinery gene
GH799_RS06155 (GH799_06150)	pilC	1206507..1207730 (-)	1224	WP_005493981.1	type II secretion system F family protein	Machinery gene
GH799_RS06160 (GH799_06155)	pilB	1207754..1209439 (-)	1686	WP_005493983.1	type IV-A pilus assembly ATPase PilB	Machinery gene
GH799_RS06165 (GH799_06160)	-	1209441..1209893 (-)	453	WP_005493986.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
GH799_RS06170 (GH799_06165)	nadC	1210157..1211044 (-)	888	WP_005493988.1	carboxylating nicotinate-nucleotide diphosphorylase	-

Sequence

Protein

• Length: 289 a.a.
• Molecular weight: 32377.43 Da
• Isoelectric Point: 7.1971

>NTDB_id=397247 GH799_RS06150 WP_005493974.1 1205573..1206442(-) (pilD) [Vibrio parahaemolyticus 10329]
MEVFQYYPWLFVVFASIFGLIVGSFLNVVIYRLPKIMELEWRRECAESFPEYKIKPPQEVLTLSVPRSSCQNCATPIRIR
DNIPVISWLLLKGKCHHCHTAISPRYPLIELLTAACAGFVAYHFGFSYFTVALIFFTFVLIAATFIDLDTMLLPDQLTLP
LTWAGIALALTEISPVSLQDAVIGAIAGYLCLWSVYWGFKLLTGKEGMGYGDFKLLAALGAWLGWQSLPMIILLSSVVGV
IFGLVQLRLQKQGIERAFPFGPYLAIAGWASLIWGDQILSWYFTSILGV

Nucleotide

• Length: 870 bp

>NTDB_id=397247 GH799_RS06150 WP_005493974.1 1205573..1206442(-) (pilD) [Vibrio parahaemolyticus 10329]
ATGGAAGTGTTCCAGTACTACCCTTGGCTATTCGTGGTGTTTGCCAGTATCTTCGGTTTAATTGTGGGTAGTTTTCTTAA
TGTAGTCATTTATCGGCTACCTAAAATCATGGAATTAGAATGGCGCCGTGAATGCGCCGAATCATTCCCTGAATATAAGA
TTAAGCCACCTCAAGAAGTTTTGACATTAAGCGTGCCTCGCTCTTCGTGCCAAAACTGCGCGACGCCAATTCGTATTCGA
GACAATATTCCTGTAATCAGCTGGCTACTGTTAAAAGGAAAATGCCATCATTGTCATACCGCAATTAGTCCTCGATATCC
GCTAATAGAATTACTCACTGCAGCGTGCGCTGGTTTTGTGGCTTATCATTTCGGCTTTAGCTACTTTACGGTTGCGTTAA
TCTTTTTCACATTTGTCTTAATAGCCGCGACTTTTATCGATCTCGATACTATGCTGCTACCGGACCAATTAACATTACCA
TTAACTTGGGCTGGCATTGCGTTAGCATTAACAGAAATAAGCCCTGTTAGTCTACAAGACGCCGTAATTGGTGCGATTGC
CGGTTATTTGTGTCTTTGGTCAGTATATTGGGGTTTCAAACTGCTTACTGGTAAAGAAGGCATGGGTTACGGTGATTTTA
AATTGCTGGCTGCCCTTGGCGCTTGGCTAGGATGGCAATCATTACCAATGATCATCCTACTTTCTTCTGTCGTCGGTGTG
ATATTCGGGCTCGTTCAGTTACGACTACAAAAACAAGGTATAGAGAGAGCTTTCCCTTTCGGCCCATATTTAGCGATTGC
AGGATGGGCAAGCTTAATCTGGGGCGATCAAATTCTAAGTTGGTACTTCACGTCAATTTTAGGAGTGTAA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (135-244)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (17-124)

3D structure

• AlphaFold DB: A0A249W3M2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio campbellii strain DS40M4	88.927	100	0.889
pilD	Vibrio cholerae strain A1552	73.611	99.654	0.734
pilD	Acinetobacter baumannii D1279779	52.239	92.734	0.484
pilD	Acinetobacter nosocomialis M2	51.866	92.734	0.481
pilD	Neisseria gonorrhoeae MS11	47.101	95.502	0.45